Melanin production and laccase mediated oxidative stress alleviation during fungal-fungal interaction among basidiomycete fungi
Abstract Fungal-fungal interaction often leads to the change in metabolite profile of both the interacting fungus which may have potential implication in industry or agriculture. In the present study, we performed two sets of fungal-fungal interaction—Trametes coccinea (F3) with Leiotrametes lactine...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-11-01
|
| Series: | IMA Fungus |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43008-021-00082-y |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832573490922782720 |
|---|---|
| author | Samim Dullah Dibya Jyoti Hazarika Gunajit Goswami Tanushree Borgohain Alokesh Ghosh Madhumita Barooah Ashok Bhattacharyya Robin Chandra Boro |
| author_facet | Samim Dullah Dibya Jyoti Hazarika Gunajit Goswami Tanushree Borgohain Alokesh Ghosh Madhumita Barooah Ashok Bhattacharyya Robin Chandra Boro |
| author_sort | Samim Dullah |
| collection | DOAJ |
| description | Abstract Fungal-fungal interaction often leads to the change in metabolite profile of both the interacting fungus which may have potential implication in industry or agriculture. In the present study, we performed two sets of fungal-fungal interaction—Trametes coccinea (F3) with Leiotrametes lactinea (F9) and T. coccinea (F3) with T. versicolor (F1) to understand the changes in the metabolite profile during the interaction process and how this process impacts the hyphal/mycelial morphology of the participating fungi. The metabolites produced during interaction of T. coccinea (F3) with L. lactinea (F9) and T. coccinea (F3) with T. versicolor (F1) was analysed through liquid chromatography coupled to mass spectroscopy (LC-MS). Most of the metabolites secreted or produced during interaction are associated with defensive response. Further, visualization with scanning electron microscopy revealed that interaction between the tested fungi led to the changes in the hyphal morphology. The bipartite fungal interaction resulted in the production of a dark brown colour pigment—melanin as confirmed by the LC-MS, FTIR and NMR analysis. Moreover, the fungal–fungal interaction also led to increase in the production of laccase, a group of multicopper oxidases involved in detoxification of toxic compounds. Further, increased activity of superoxide dismutase, an enzyme that catalyzes the dismutation of the superoxide anion to hydrogen peroxide was also recorded during fungal–fungal interaction. Quantitative real-time PCR revealed upregulation of lcc1 (encoding a laccase enzyme) and few other stress related genes of T. versicolor during its hyphal interaction with T. coccinea, suggesting a direct correlation between laccase production and melanin production. |
| format | Article |
| id | doaj-art-7a772e9470ef432b9f79c67e721e3953 |
| institution | Kabale University |
| issn | 2210-6359 |
| language | English |
| publishDate | 2021-11-01 |
| publisher | BMC |
| record_format | Article |
| series | IMA Fungus |
| spelling | doaj-art-7a772e9470ef432b9f79c67e721e39532025-02-02T04:00:49ZengBMCIMA Fungus2210-63592021-11-0112111710.1186/s43008-021-00082-yMelanin production and laccase mediated oxidative stress alleviation during fungal-fungal interaction among basidiomycete fungiSamim Dullah0Dibya Jyoti Hazarika1Gunajit Goswami2Tanushree Borgohain3Alokesh Ghosh4Madhumita Barooah5Ashok Bhattacharyya6Robin Chandra Boro7Department of Agricultural Biotechnology, Assam Agricultural UniversityDepartment of Agricultural Biotechnology, Assam Agricultural UniversityDBT-North East Centre for Agricultural Biotechnology, Assam Agricultural UniversityDepartment of Agricultural Biotechnology, Assam Agricultural UniversityDepartment of Agricultural Biotechnology, Assam Agricultural UniversityDepartment of Agricultural Biotechnology, Assam Agricultural UniversityDepartment of Plant Pathology, Assam Agricultural UniversityDepartment of Agricultural Biotechnology, Assam Agricultural UniversityAbstract Fungal-fungal interaction often leads to the change in metabolite profile of both the interacting fungus which may have potential implication in industry or agriculture. In the present study, we performed two sets of fungal-fungal interaction—Trametes coccinea (F3) with Leiotrametes lactinea (F9) and T. coccinea (F3) with T. versicolor (F1) to understand the changes in the metabolite profile during the interaction process and how this process impacts the hyphal/mycelial morphology of the participating fungi. The metabolites produced during interaction of T. coccinea (F3) with L. lactinea (F9) and T. coccinea (F3) with T. versicolor (F1) was analysed through liquid chromatography coupled to mass spectroscopy (LC-MS). Most of the metabolites secreted or produced during interaction are associated with defensive response. Further, visualization with scanning electron microscopy revealed that interaction between the tested fungi led to the changes in the hyphal morphology. The bipartite fungal interaction resulted in the production of a dark brown colour pigment—melanin as confirmed by the LC-MS, FTIR and NMR analysis. Moreover, the fungal–fungal interaction also led to increase in the production of laccase, a group of multicopper oxidases involved in detoxification of toxic compounds. Further, increased activity of superoxide dismutase, an enzyme that catalyzes the dismutation of the superoxide anion to hydrogen peroxide was also recorded during fungal–fungal interaction. Quantitative real-time PCR revealed upregulation of lcc1 (encoding a laccase enzyme) and few other stress related genes of T. versicolor during its hyphal interaction with T. coccinea, suggesting a direct correlation between laccase production and melanin production.https://doi.org/10.1186/s43008-021-00082-yHyphal interactionsLaccaseMelaninNMR spectroscopySuperoxide dismutaseROS |
| spellingShingle | Samim Dullah Dibya Jyoti Hazarika Gunajit Goswami Tanushree Borgohain Alokesh Ghosh Madhumita Barooah Ashok Bhattacharyya Robin Chandra Boro Melanin production and laccase mediated oxidative stress alleviation during fungal-fungal interaction among basidiomycete fungi IMA Fungus Hyphal interactions Laccase Melanin NMR spectroscopy Superoxide dismutase ROS |
| title | Melanin production and laccase mediated oxidative stress alleviation during fungal-fungal interaction among basidiomycete fungi |
| title_full | Melanin production and laccase mediated oxidative stress alleviation during fungal-fungal interaction among basidiomycete fungi |
| title_fullStr | Melanin production and laccase mediated oxidative stress alleviation during fungal-fungal interaction among basidiomycete fungi |
| title_full_unstemmed | Melanin production and laccase mediated oxidative stress alleviation during fungal-fungal interaction among basidiomycete fungi |
| title_short | Melanin production and laccase mediated oxidative stress alleviation during fungal-fungal interaction among basidiomycete fungi |
| title_sort | melanin production and laccase mediated oxidative stress alleviation during fungal fungal interaction among basidiomycete fungi |
| topic | Hyphal interactions Laccase Melanin NMR spectroscopy Superoxide dismutase ROS |
| url | https://doi.org/10.1186/s43008-021-00082-y |
| work_keys_str_mv | AT samimdullah melaninproductionandlaccasemediatedoxidativestressalleviationduringfungalfungalinteractionamongbasidiomycetefungi AT dibyajyotihazarika melaninproductionandlaccasemediatedoxidativestressalleviationduringfungalfungalinteractionamongbasidiomycetefungi AT gunajitgoswami melaninproductionandlaccasemediatedoxidativestressalleviationduringfungalfungalinteractionamongbasidiomycetefungi AT tanushreeborgohain melaninproductionandlaccasemediatedoxidativestressalleviationduringfungalfungalinteractionamongbasidiomycetefungi AT alokeshghosh melaninproductionandlaccasemediatedoxidativestressalleviationduringfungalfungalinteractionamongbasidiomycetefungi AT madhumitabarooah melaninproductionandlaccasemediatedoxidativestressalleviationduringfungalfungalinteractionamongbasidiomycetefungi AT ashokbhattacharyya melaninproductionandlaccasemediatedoxidativestressalleviationduringfungalfungalinteractionamongbasidiomycetefungi AT robinchandraboro melaninproductionandlaccasemediatedoxidativestressalleviationduringfungalfungalinteractionamongbasidiomycetefungi |