A novel barcoded nanopore sequencing workflow of high-quality, full-length bacterial 16S amplicons for taxonomic annotation of bacterial isolates and complex microbial communities

ABSTRACT Due to recent improvements, Nanopore sequencing has become a promising method for experiments relying on amplicon sequencing. We describe a flexible workflow to generate and annotate high-quality, full-length 16S rDNA amplicons. We evaluated it for two applications, namely, (i) identificati...

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Bibliographic Details
Main Authors: Julian Dommann, Jakob Kerbl-Knapp, Diana Albertos Torres, Adrian Egli, Jennifer Keiser, Pierre H. H. Schneeberger
Format: Article
Language:English
Published: American Society for Microbiology 2024-10-01
Series:mSystems
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Online Access:https://journals.asm.org/doi/10.1128/msystems.00859-24
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