Using Gap Visualization to Navigate Multivariate Metabarcode Data, Select Primer Pairs, and Enhance Reference Data Quality

Using Gap Visualization to Navigate Multivariate Metabarcode Data, Select Primer Pairs, and Enhance Reference Data Quality

ABSTRACT The success of amplicon sequencing studies of environmental DNA (eDNA) depends on the choice of primer pairs used to select taxa‐specific regions of DNA for amplification then sequencing to characterize sample composition. This paper presents practical methods to visualize the extent to whi...

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Bibliographic Details
Main Authors: Xin‐Yi Chua, Louise Ord, Stephen J. Bent, David Lovell, Annette McGrath
Format: Article
Language:English
Published: Wiley 2024-11-01
Series:Environmental DNA
Subjects:
amplicon sequencing
metabarcoding
metabarcoding gaps
taxonomic resolution
visualization
Online Access:https://doi.org/10.1002/edn3.597
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https://doi.org/10.1002/edn3.597

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