Bioseparation of Four Proteins from Euphorbia characias Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase
This paper deals with the purification of four proteins from Euphorbia characias latex, a copper amine oxidase, a nucleotide pyrophosphatase/phosphodiesterase, a peroxidase, and a purple acid phosphatase. These proteins, very different in molecular weight, in primary structure, and in the catalyzed...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2011/369484 |
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| author | Rosaria Medda Francesca Pintus Delia Spanò Giovanni Floris |
| author_facet | Rosaria Medda Francesca Pintus Delia Spanò Giovanni Floris |
| author_sort | Rosaria Medda |
| collection | DOAJ |
| description | This paper deals with the purification of four proteins from Euphorbia characias latex, a copper amine oxidase, a nucleotide pyrophosphatase/phosphodiesterase, a peroxidase, and a purple acid phosphatase. These proteins, very different in molecular weight, in primary structure, and in the catalyzed reaction, are purified using identical preliminary steps of purification and by chromatographic methods. In particular, the DEAE-cellulose chromatography is used as a useful purification step for all the four enzymes. The purification methods here reported allow to obtain a high purification of all the four proteins with a good yield. This paper will give some thorough suggestions for researchers busy in separation of macromolecules from different sources. |
| format | Article |
| id | doaj-art-78f184d80d0c4fc593624edeada0c516 |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-78f184d80d0c4fc593624edeada0c5162025-02-03T01:24:26ZengWileyBiochemistry Research International2090-22472090-22552011-01-01201110.1155/2011/369484369484Bioseparation of Four Proteins from Euphorbia characias Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid PhosphataseRosaria Medda0Francesca Pintus1Delia Spanò2Giovanni Floris3Dipartimento di Scienze della Vita e dell'Ambiente, Università di Cagliari, Cittadella Universitaria, 09042 Monserrato, ItalyDipartimento di Scienze della Vita e dell'Ambiente, Università di Cagliari, Cittadella Universitaria, 09042 Monserrato, ItalyDipartimento di Scienze della Vita e dell'Ambiente, Università di Cagliari, Cittadella Universitaria, 09042 Monserrato, ItalyDipartimento di Scienze della Vita e dell'Ambiente, Università di Cagliari, Cittadella Universitaria, 09042 Monserrato, ItalyThis paper deals with the purification of four proteins from Euphorbia characias latex, a copper amine oxidase, a nucleotide pyrophosphatase/phosphodiesterase, a peroxidase, and a purple acid phosphatase. These proteins, very different in molecular weight, in primary structure, and in the catalyzed reaction, are purified using identical preliminary steps of purification and by chromatographic methods. In particular, the DEAE-cellulose chromatography is used as a useful purification step for all the four enzymes. The purification methods here reported allow to obtain a high purification of all the four proteins with a good yield. This paper will give some thorough suggestions for researchers busy in separation of macromolecules from different sources.http://dx.doi.org/10.1155/2011/369484 |
| spellingShingle | Rosaria Medda Francesca Pintus Delia Spanò Giovanni Floris Bioseparation of Four Proteins from Euphorbia characias Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase Biochemistry Research International |
| title | Bioseparation of Four Proteins from Euphorbia characias Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase |
| title_full | Bioseparation of Four Proteins from Euphorbia characias Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase |
| title_fullStr | Bioseparation of Four Proteins from Euphorbia characias Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase |
| title_full_unstemmed | Bioseparation of Four Proteins from Euphorbia characias Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase |
| title_short | Bioseparation of Four Proteins from Euphorbia characias Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase |
| title_sort | bioseparation of four proteins from euphorbia characias latex amine oxidase peroxidase nucleotide pyrophosphatase phosphodiesterase and purple acid phosphatase |
| url | http://dx.doi.org/10.1155/2011/369484 |
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