Electrochemical Analysis of Antichemotherapeutic Drug Zanosar in Pharmaceutical and Biological Samples by Differential Pulse Polarography
The electrochemical reduction of zanosar was investigated systematically by direct current polarography, cyclic voltammetry, and differential pulse polarography (DPP). A simple DPP technique was proposed for the direct quantitative determination of anticancer drug zanosar in pharmaceutical formulati...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/420761 |
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| author | Chennupalle Nageswara Reddy Puthalapattu ReddyPrasad NeelamYughandhar Sreedhar |
| author_facet | Chennupalle Nageswara Reddy Puthalapattu ReddyPrasad NeelamYughandhar Sreedhar |
| author_sort | Chennupalle Nageswara Reddy |
| collection | DOAJ |
| description | The electrochemical reduction of zanosar was investigated systematically by direct current polarography, cyclic voltammetry, and differential pulse polarography (DPP). A simple DPP technique was proposed for the direct quantitative determination of anticancer drug zanosar in pharmaceutical formulation and spiked human urine samples for the first time. The reduction potential was −0.28 V versus Ag/AgCl with a hanging mercury drop electrode in Britton-Robinson buffer as supporting electrolyte. The dependence of the intensities of currents and potentials on pH, concentration, scan rate, deposition time, and nature of the supporting electrolyte was investigated. The calibration curve was found to be linear with the following equation: y=0.4041x+0.012, with a correlation coefficient of 0.992 (R2) over a concentration range from 1.0×10-7 M to 1.0×10-3 M. In the present investigation, the achieved limit of detection (LOD) and limit of quantization (LQD) were 7.42×10-8 M and 2.47×10-8 M; respectively. Excipients did not interfere with the determination of zanosar in pharmaceutical formulation and spiked urine samples. Precision and accuracy of the developed method were checked by recovery studies in pharmaceutical formulation and spiked human urine samples. |
| format | Article |
| id | doaj-art-7834a4b0f7ee4f18b6276329cdb4d151 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-7834a4b0f7ee4f18b6276329cdb4d1512025-02-03T01:21:19ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732013-01-01201310.1155/2013/420761420761Electrochemical Analysis of Antichemotherapeutic Drug Zanosar in Pharmaceutical and Biological Samples by Differential Pulse PolarographyChennupalle Nageswara Reddy0Puthalapattu ReddyPrasad1NeelamYughandhar Sreedhar2Department of Chemistry, Government Degree College, Kodur, Kadapa District, Andhra Pradesh, IndiaGraduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 10607, TaiwanElectroanalytical Lab, Department of Chemistry, Sri Venkateswara University, Tirupati, Andhra Pradesh, IndiaThe electrochemical reduction of zanosar was investigated systematically by direct current polarography, cyclic voltammetry, and differential pulse polarography (DPP). A simple DPP technique was proposed for the direct quantitative determination of anticancer drug zanosar in pharmaceutical formulation and spiked human urine samples for the first time. The reduction potential was −0.28 V versus Ag/AgCl with a hanging mercury drop electrode in Britton-Robinson buffer as supporting electrolyte. The dependence of the intensities of currents and potentials on pH, concentration, scan rate, deposition time, and nature of the supporting electrolyte was investigated. The calibration curve was found to be linear with the following equation: y=0.4041x+0.012, with a correlation coefficient of 0.992 (R2) over a concentration range from 1.0×10-7 M to 1.0×10-3 M. In the present investigation, the achieved limit of detection (LOD) and limit of quantization (LQD) were 7.42×10-8 M and 2.47×10-8 M; respectively. Excipients did not interfere with the determination of zanosar in pharmaceutical formulation and spiked urine samples. Precision and accuracy of the developed method were checked by recovery studies in pharmaceutical formulation and spiked human urine samples.http://dx.doi.org/10.1155/2013/420761 |
| spellingShingle | Chennupalle Nageswara Reddy Puthalapattu ReddyPrasad NeelamYughandhar Sreedhar Electrochemical Analysis of Antichemotherapeutic Drug Zanosar in Pharmaceutical and Biological Samples by Differential Pulse Polarography Journal of Analytical Methods in Chemistry |
| title | Electrochemical Analysis of Antichemotherapeutic Drug Zanosar in Pharmaceutical and Biological Samples by Differential Pulse Polarography |
| title_full | Electrochemical Analysis of Antichemotherapeutic Drug Zanosar in Pharmaceutical and Biological Samples by Differential Pulse Polarography |
| title_fullStr | Electrochemical Analysis of Antichemotherapeutic Drug Zanosar in Pharmaceutical and Biological Samples by Differential Pulse Polarography |
| title_full_unstemmed | Electrochemical Analysis of Antichemotherapeutic Drug Zanosar in Pharmaceutical and Biological Samples by Differential Pulse Polarography |
| title_short | Electrochemical Analysis of Antichemotherapeutic Drug Zanosar in Pharmaceutical and Biological Samples by Differential Pulse Polarography |
| title_sort | electrochemical analysis of antichemotherapeutic drug zanosar in pharmaceutical and biological samples by differential pulse polarography |
| url | http://dx.doi.org/10.1155/2013/420761 |
| work_keys_str_mv | AT chennupallenageswarareddy electrochemicalanalysisofantichemotherapeuticdrugzanosarinpharmaceuticalandbiologicalsamplesbydifferentialpulsepolarography AT puthalapattureddyprasad electrochemicalanalysisofantichemotherapeuticdrugzanosarinpharmaceuticalandbiologicalsamplesbydifferentialpulsepolarography AT neelamyughandharsreedhar electrochemicalanalysisofantichemotherapeuticdrugzanosarinpharmaceuticalandbiologicalsamplesbydifferentialpulsepolarography |