Validated HPTLC Method for Quantification of Luteolin and Apigenin in Premna mucronata Roxb., Verbenaceae
A simple, rapid, and precise high-performance thin-layer chromatographic method was developed for quantitative estimation of luteolin and apigenin in Premna mucronata Roxb., family Verbenaceae. Separation was performed on silica gel 60 F254 HPTLC plates using toluene : ethyl acetate : formic acid (6...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Advances in Pharmacological Sciences |
| Online Access: | http://dx.doi.org/10.1155/2015/682365 |
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| author | Nayan G. Patel Kalpana G. Patel Kirti V. Patel Tejal R. Gandhi |
| author_facet | Nayan G. Patel Kalpana G. Patel Kirti V. Patel Tejal R. Gandhi |
| author_sort | Nayan G. Patel |
| collection | DOAJ |
| description | A simple, rapid, and precise high-performance thin-layer chromatographic method was developed for quantitative estimation of luteolin and apigenin in Premna mucronata Roxb., family Verbenaceae. Separation was performed on silica gel 60 F254 HPTLC plates using toluene : ethyl acetate : formic acid (6 : 4 : 0.3) as mobile phase for elution of markers from extract. The determination was carried out in fluorescence mode using densitometric absorbance-reflection mode at 366 nm for both luteolin and apigenin. The methanolic extract of Premna mucronata was found to contain 10.2 mg/g % luteolin and 0.165 mg/g % of apigenin. The method was validated in terms of linearity, LOD and LOQ, accuracy, precision, and specificity. The calibration curve was found to be linear between 200 and 1000 ng/band for luteolin and 50 and 250 ng/band for apigenin. For luteolin and apigenin, the limit of detection was found to be 42.6 ng/band and 7.97 ng/band while the limit of quantitation was found to be 129.08 ng/band and 24.155 ng/band, respectively. This developed validated method is capable of quantifying and resolving luteolin and apigenin and can be applicable for routine analysis of extract and plant as a whole. |
| format | Article |
| id | doaj-art-77909566d69e44cda4db9fd8a2662eba |
| institution | Kabale University |
| issn | 1687-6334 1687-6342 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Pharmacological Sciences |
| spelling | doaj-art-77909566d69e44cda4db9fd8a2662eba2025-02-03T01:11:35ZengWileyAdvances in Pharmacological Sciences1687-63341687-63422015-01-01201510.1155/2015/682365682365Validated HPTLC Method for Quantification of Luteolin and Apigenin in Premna mucronata Roxb., VerbenaceaeNayan G. Patel0Kalpana G. Patel1Kirti V. Patel2Tejal R. Gandhi3Department of Pharmacology and Toxicology, Faculty of Pharmacy, Dharmsinh Desai University, College Road, Nadiad, Gujarat 387001, IndiaDepartment of Quality Assurance, Anand Pharmacy College, Shri Ram Krishna Seva Mandal Campus, Near Town Hall, Anand, Gujarat 388001, IndiaDepartment of Pharmacology, Pharmacy Department, The MS University of Baroda, Vadodara, Gujarat 390002, IndiaDepartment of Pharmacology, Anand Pharmacy College, Shri Ram Krishna Seva Mandal Campus, Near Town Hall, Anand, Gujarat 388001, IndiaA simple, rapid, and precise high-performance thin-layer chromatographic method was developed for quantitative estimation of luteolin and apigenin in Premna mucronata Roxb., family Verbenaceae. Separation was performed on silica gel 60 F254 HPTLC plates using toluene : ethyl acetate : formic acid (6 : 4 : 0.3) as mobile phase for elution of markers from extract. The determination was carried out in fluorescence mode using densitometric absorbance-reflection mode at 366 nm for both luteolin and apigenin. The methanolic extract of Premna mucronata was found to contain 10.2 mg/g % luteolin and 0.165 mg/g % of apigenin. The method was validated in terms of linearity, LOD and LOQ, accuracy, precision, and specificity. The calibration curve was found to be linear between 200 and 1000 ng/band for luteolin and 50 and 250 ng/band for apigenin. For luteolin and apigenin, the limit of detection was found to be 42.6 ng/band and 7.97 ng/band while the limit of quantitation was found to be 129.08 ng/band and 24.155 ng/band, respectively. This developed validated method is capable of quantifying and resolving luteolin and apigenin and can be applicable for routine analysis of extract and plant as a whole.http://dx.doi.org/10.1155/2015/682365 |
| spellingShingle | Nayan G. Patel Kalpana G. Patel Kirti V. Patel Tejal R. Gandhi Validated HPTLC Method for Quantification of Luteolin and Apigenin in Premna mucronata Roxb., Verbenaceae Advances in Pharmacological Sciences |
| title | Validated HPTLC Method for Quantification of Luteolin and Apigenin in Premna mucronata Roxb., Verbenaceae |
| title_full | Validated HPTLC Method for Quantification of Luteolin and Apigenin in Premna mucronata Roxb., Verbenaceae |
| title_fullStr | Validated HPTLC Method for Quantification of Luteolin and Apigenin in Premna mucronata Roxb., Verbenaceae |
| title_full_unstemmed | Validated HPTLC Method for Quantification of Luteolin and Apigenin in Premna mucronata Roxb., Verbenaceae |
| title_short | Validated HPTLC Method for Quantification of Luteolin and Apigenin in Premna mucronata Roxb., Verbenaceae |
| title_sort | validated hptlc method for quantification of luteolin and apigenin in premna mucronata roxb verbenaceae |
| url | http://dx.doi.org/10.1155/2015/682365 |
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