LPCAT3 regulates the proliferation and metastasis of serous ovarian cancer by modulating arachidonic acid
Background: Lysophosphatidylcholine acyltransferase 3 (LPCAT3) promotes ferroptosis through the incorporating polyunsaturated fatty acids into membrane phospholipids, however, its role in serous ovarian cancer remains unclear. Here explored cancer proliferation and metastasis after modulating LPCAP3...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Translational Oncology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1936523324003826 |
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| author | Fang Wen Hongjian Ling Rui Ran Xinya Li Houmei Wang Qianfen Liu Min Li Tinghe Yu |
| author_facet | Fang Wen Hongjian Ling Rui Ran Xinya Li Houmei Wang Qianfen Liu Min Li Tinghe Yu |
| author_sort | Fang Wen |
| collection | DOAJ |
| description | Background: Lysophosphatidylcholine acyltransferase 3 (LPCAT3) promotes ferroptosis through the incorporating polyunsaturated fatty acids into membrane phospholipids, however, its role in serous ovarian cancer remains unclear. Here explored cancer proliferation and metastasis after modulating LPCAP3. Methods: LPCAT3 protein in ovarian cancer tissues was detected using bioinformatic and immunohistoche mical assays. Cell behaviors were observed after up- or down-regulating LPCAT3. Lipid metabolites were determined, and then the pathway enrichment analysis was performed. Results: The expression level of LPCAT3 in serous ovarian cancer tissues was lower than that in other types of ovarian cancer, and high expression was associated with a longer survival time. Overexpressing LPCAT3 reduced cell proliferation, migration and invasion via enhancing ferroptosis and decreasing the survival signaling; these behaviors were enhanced in LPCAT3-downknocked cells, where a higher abundance of arachidonic acid was observed followed by up-regulation of the downstream survival signaling. In vivo, up-regulation of LPCAT3 decreased tumor growth, but down-regulation enhanced tumor growth and metastasis. Conclusions: LPCAT3 modulated metabolism of arachidonic acid, thereby regulating ferroptosis and the survival signaling to determine cancer growth and metastasis. |
| format | Article |
| id | doaj-art-773dc72e873047e3bcce6c8215255b5a |
| institution | Kabale University |
| issn | 1936-5233 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Oncology |
| spelling | doaj-art-773dc72e873047e3bcce6c8215255b5a2025-01-22T05:41:30ZengElsevierTranslational Oncology1936-52332025-02-0152102256LPCAT3 regulates the proliferation and metastasis of serous ovarian cancer by modulating arachidonic acidFang Wen0Hongjian Ling1Rui Ran2Xinya Li3Houmei Wang4Qianfen Liu5Min Li6Tinghe Yu7Laboratory of Obstetrics and Gynecology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, ChinaLaboratory of Obstetrics and Gynecology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, ChinaLaboratory of Obstetrics and Gynecology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, ChinaLaboratory of Obstetrics and Gynecology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, ChinaLaboratory of Obstetrics and Gynecology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, ChinaWomen and Children's Hospital, Chongqing Medical University (Chongqing Health Center for Women and Children), ChinaLaboratory of Obstetrics and Gynecology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China; Corresponding author.Laboratory of Obstetrics and Gynecology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China; Corresponding author.Background: Lysophosphatidylcholine acyltransferase 3 (LPCAT3) promotes ferroptosis through the incorporating polyunsaturated fatty acids into membrane phospholipids, however, its role in serous ovarian cancer remains unclear. Here explored cancer proliferation and metastasis after modulating LPCAP3. Methods: LPCAT3 protein in ovarian cancer tissues was detected using bioinformatic and immunohistoche mical assays. Cell behaviors were observed after up- or down-regulating LPCAT3. Lipid metabolites were determined, and then the pathway enrichment analysis was performed. Results: The expression level of LPCAT3 in serous ovarian cancer tissues was lower than that in other types of ovarian cancer, and high expression was associated with a longer survival time. Overexpressing LPCAT3 reduced cell proliferation, migration and invasion via enhancing ferroptosis and decreasing the survival signaling; these behaviors were enhanced in LPCAT3-downknocked cells, where a higher abundance of arachidonic acid was observed followed by up-regulation of the downstream survival signaling. In vivo, up-regulation of LPCAT3 decreased tumor growth, but down-regulation enhanced tumor growth and metastasis. Conclusions: LPCAT3 modulated metabolism of arachidonic acid, thereby regulating ferroptosis and the survival signaling to determine cancer growth and metastasis.http://www.sciencedirect.com/science/article/pii/S1936523324003826Ovarian cancerLPCAT3Lipid metabolismArachidonic acidFerroptosis |
| spellingShingle | Fang Wen Hongjian Ling Rui Ran Xinya Li Houmei Wang Qianfen Liu Min Li Tinghe Yu LPCAT3 regulates the proliferation and metastasis of serous ovarian cancer by modulating arachidonic acid Translational Oncology Ovarian cancer LPCAT3 Lipid metabolism Arachidonic acid Ferroptosis |
| title | LPCAT3 regulates the proliferation and metastasis of serous ovarian cancer by modulating arachidonic acid |
| title_full | LPCAT3 regulates the proliferation and metastasis of serous ovarian cancer by modulating arachidonic acid |
| title_fullStr | LPCAT3 regulates the proliferation and metastasis of serous ovarian cancer by modulating arachidonic acid |
| title_full_unstemmed | LPCAT3 regulates the proliferation and metastasis of serous ovarian cancer by modulating arachidonic acid |
| title_short | LPCAT3 regulates the proliferation and metastasis of serous ovarian cancer by modulating arachidonic acid |
| title_sort | lpcat3 regulates the proliferation and metastasis of serous ovarian cancer by modulating arachidonic acid |
| topic | Ovarian cancer LPCAT3 Lipid metabolism Arachidonic acid Ferroptosis |
| url | http://www.sciencedirect.com/science/article/pii/S1936523324003826 |
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