Analysis of the Control Effect of <i>Bacillus amyloliquefaciens</i> C4 Wettable Powder on Potato Bacterial Wilt Caused by <i>Ralstonia solanacearum</i>
Potatoes are one of the most important food crops worldwide, but their growth and development are often seriously threatened by potato bacterial wilt. The wettable powder produced by <i>Bacillus amyloliquefaciens</i> C4 under optimized fermentation conditions effectively inhibits potato...
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MDPI AG
2025-01-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/15/1/206 |
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| author | Zhixiang Xing Dan Liu Meng Luo Zelin Yang Wenyuan Pang Yexing Feng Jiani Yan Fumeng He Xu Feng Qiang Yuan Yingnan Wang Fenglan Li |
| author_facet | Zhixiang Xing Dan Liu Meng Luo Zelin Yang Wenyuan Pang Yexing Feng Jiani Yan Fumeng He Xu Feng Qiang Yuan Yingnan Wang Fenglan Li |
| author_sort | Zhixiang Xing |
| collection | DOAJ |
| description | Potatoes are one of the most important food crops worldwide, but their growth and development are often seriously threatened by potato bacterial wilt. The wettable powder produced by <i>Bacillus amyloliquefaciens</i> C4 under optimized fermentation conditions effectively inhibits potato bacterial wilt. In this study, lipopeptide antibiotics were identified via PCR and MALDI-TOF-MS, and their antibacterial activity was determined. The optimal formulation of C4 wettable powder was optimized via a single-factor experiment combined with a response surface. The effect of C4 wettable powder on potato bacterial wilt was evaluated. In the antibacterial activity test, surfactin showed better inhibition ability. After determining the optimal liquid fermentation conditions and wettable powder formula, the surfactin activity increased to 540.15 mg/L, and the C4 wettable powder activity reached 69.67 × 10<sup>8</sup> cfu/g. The results of the pot experiment showed that the best cost-effectiveness was achieved under 500 times dilution and spraying, with a control effect of 79.05 ± 24.79%. The physiological and biochemical results showed that C4 wettable powder could induce rapid defense enzyme responses in leaves and enhance plant resistance to pathogenic bacteria. The results showed that C4 wettable powder effectively controlled potato bacterial wilt, and its application method was determined. |
| format | Article |
| id | doaj-art-771f60f389814ca48c9d447eed803d84 |
| institution | Kabale University |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj-art-771f60f389814ca48c9d447eed803d842025-01-24T13:17:10ZengMDPI AGAgronomy2073-43952025-01-0115120610.3390/agronomy15010206Analysis of the Control Effect of <i>Bacillus amyloliquefaciens</i> C4 Wettable Powder on Potato Bacterial Wilt Caused by <i>Ralstonia solanacearum</i>Zhixiang Xing0Dan Liu1Meng Luo2Zelin Yang3Wenyuan Pang4Yexing Feng5Jiani Yan6Fumeng He7Xu Feng8Qiang Yuan9Yingnan Wang10Fenglan Li11College of Life Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Science, Northeast Agricultural University, Harbin 150030, ChinaPotatoes are one of the most important food crops worldwide, but their growth and development are often seriously threatened by potato bacterial wilt. The wettable powder produced by <i>Bacillus amyloliquefaciens</i> C4 under optimized fermentation conditions effectively inhibits potato bacterial wilt. In this study, lipopeptide antibiotics were identified via PCR and MALDI-TOF-MS, and their antibacterial activity was determined. The optimal formulation of C4 wettable powder was optimized via a single-factor experiment combined with a response surface. The effect of C4 wettable powder on potato bacterial wilt was evaluated. In the antibacterial activity test, surfactin showed better inhibition ability. After determining the optimal liquid fermentation conditions and wettable powder formula, the surfactin activity increased to 540.15 mg/L, and the C4 wettable powder activity reached 69.67 × 10<sup>8</sup> cfu/g. The results of the pot experiment showed that the best cost-effectiveness was achieved under 500 times dilution and spraying, with a control effect of 79.05 ± 24.79%. The physiological and biochemical results showed that C4 wettable powder could induce rapid defense enzyme responses in leaves and enhance plant resistance to pathogenic bacteria. The results showed that C4 wettable powder effectively controlled potato bacterial wilt, and its application method was determined.https://www.mdpi.com/2073-4395/15/1/206potato bacterial wilt<i>Bacillus amyloliquefaciens</i> C4surfactinwettable powderbiocontrol agents |
| spellingShingle | Zhixiang Xing Dan Liu Meng Luo Zelin Yang Wenyuan Pang Yexing Feng Jiani Yan Fumeng He Xu Feng Qiang Yuan Yingnan Wang Fenglan Li Analysis of the Control Effect of <i>Bacillus amyloliquefaciens</i> C4 Wettable Powder on Potato Bacterial Wilt Caused by <i>Ralstonia solanacearum</i> Agronomy potato bacterial wilt <i>Bacillus amyloliquefaciens</i> C4 surfactin wettable powder biocontrol agents |
| title | Analysis of the Control Effect of <i>Bacillus amyloliquefaciens</i> C4 Wettable Powder on Potato Bacterial Wilt Caused by <i>Ralstonia solanacearum</i> |
| title_full | Analysis of the Control Effect of <i>Bacillus amyloliquefaciens</i> C4 Wettable Powder on Potato Bacterial Wilt Caused by <i>Ralstonia solanacearum</i> |
| title_fullStr | Analysis of the Control Effect of <i>Bacillus amyloliquefaciens</i> C4 Wettable Powder on Potato Bacterial Wilt Caused by <i>Ralstonia solanacearum</i> |
| title_full_unstemmed | Analysis of the Control Effect of <i>Bacillus amyloliquefaciens</i> C4 Wettable Powder on Potato Bacterial Wilt Caused by <i>Ralstonia solanacearum</i> |
| title_short | Analysis of the Control Effect of <i>Bacillus amyloliquefaciens</i> C4 Wettable Powder on Potato Bacterial Wilt Caused by <i>Ralstonia solanacearum</i> |
| title_sort | analysis of the control effect of i bacillus amyloliquefaciens i c4 wettable powder on potato bacterial wilt caused by i ralstonia solanacearum i |
| topic | potato bacterial wilt <i>Bacillus amyloliquefaciens</i> C4 surfactin wettable powder biocontrol agents |
| url | https://www.mdpi.com/2073-4395/15/1/206 |
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