A novel lectin from mushroom Phellodon melaleucus displays hemagglutination activity, and antitumor activity in a B16 melanoma mouse model
A novel lectin (termed PML) was purified from fruiting bodies of the edible mushroom Phellodon melaleucus (division Basidiomycota) by ion exchange, hydrophobic interaction, and gel filtration chromatographies, with overall titer recovery ~60 % and 20-fold purification. PML displayed hemagglutination...
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Tsinghua University Press
2023-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221345302300040X |
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| author | Yuanhui Li Peng Wang Zejun Zhang Qinghong Liu |
| author_facet | Yuanhui Li Peng Wang Zejun Zhang Qinghong Liu |
| author_sort | Yuanhui Li |
| collection | DOAJ |
| description | A novel lectin (termed PML) was purified from fruiting bodies of the edible mushroom Phellodon melaleucus (division Basidiomycota) by ion exchange, hydrophobic interaction, and gel filtration chromatographies, with overall titer recovery ~60 % and 20-fold purification. PML displayed hemagglutination activity 13319 units/mg toward rabbit erythrocytes. SDS-PAGE and gel filtration analyses revealed that PML is a homodimeric lectin with a molecular weight of 28.8 kDa. PML hemagglutination activity was not inhibited by various simple sugars or their derivatives, but was enhanced by cations Ca2+, Mg2+, Zn2+, and Cu2+. The activity was stable in pH range 6–9 and in the temperature range 20–60 °C. Circular dichroism (CD) spectroscopic analysis showed that PML was composed primarily of β-sheets with low α-helix content. In a B16 melanoma mouse model, PML treatment significantly inhibited tumor growth, and increased cytokine IL-10 content. Our findings suggest that PML is a potential anticancer therapeutic agent. |
| format | Article |
| id | doaj-art-76c62ea6fb0f4065bdbeea27c5ba8d76 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-76c62ea6fb0f4065bdbeea27c5ba8d762025-02-03T05:13:19ZengTsinghua University PressFood Science and Human Wellness2213-45302023-09-0112518851892A novel lectin from mushroom Phellodon melaleucus displays hemagglutination activity, and antitumor activity in a B16 melanoma mouse modelYuanhui Li0Peng Wang1Zejun Zhang2Qinghong Liu3Department of Vegetables, College of Horticulture, China Agricultural University, Beijing 100193, ChinaDepartment of Vegetables, College of Horticulture, China Agricultural University, Beijing 100193, ChinaCollege of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100193, ChinaDepartment of Vegetables, College of Horticulture, China Agricultural University, Beijing 100193, China; Corresponding author at: Department of Vegetables, College of Horticulture, China Agricultural University, Beijing 100193, China.A novel lectin (termed PML) was purified from fruiting bodies of the edible mushroom Phellodon melaleucus (division Basidiomycota) by ion exchange, hydrophobic interaction, and gel filtration chromatographies, with overall titer recovery ~60 % and 20-fold purification. PML displayed hemagglutination activity 13319 units/mg toward rabbit erythrocytes. SDS-PAGE and gel filtration analyses revealed that PML is a homodimeric lectin with a molecular weight of 28.8 kDa. PML hemagglutination activity was not inhibited by various simple sugars or their derivatives, but was enhanced by cations Ca2+, Mg2+, Zn2+, and Cu2+. The activity was stable in pH range 6–9 and in the temperature range 20–60 °C. Circular dichroism (CD) spectroscopic analysis showed that PML was composed primarily of β-sheets with low α-helix content. In a B16 melanoma mouse model, PML treatment significantly inhibited tumor growth, and increased cytokine IL-10 content. Our findings suggest that PML is a potential anticancer therapeutic agent.http://www.sciencedirect.com/science/article/pii/S221345302300040XPhellodon melaleucusLectinHemagglutination activityCytokine IL-10Antitumor activity |
| spellingShingle | Yuanhui Li Peng Wang Zejun Zhang Qinghong Liu A novel lectin from mushroom Phellodon melaleucus displays hemagglutination activity, and antitumor activity in a B16 melanoma mouse model Food Science and Human Wellness Phellodon melaleucus Lectin Hemagglutination activity Cytokine IL-10 Antitumor activity |
| title | A novel lectin from mushroom Phellodon melaleucus displays hemagglutination activity, and antitumor activity in a B16 melanoma mouse model |
| title_full | A novel lectin from mushroom Phellodon melaleucus displays hemagglutination activity, and antitumor activity in a B16 melanoma mouse model |
| title_fullStr | A novel lectin from mushroom Phellodon melaleucus displays hemagglutination activity, and antitumor activity in a B16 melanoma mouse model |
| title_full_unstemmed | A novel lectin from mushroom Phellodon melaleucus displays hemagglutination activity, and antitumor activity in a B16 melanoma mouse model |
| title_short | A novel lectin from mushroom Phellodon melaleucus displays hemagglutination activity, and antitumor activity in a B16 melanoma mouse model |
| title_sort | novel lectin from mushroom phellodon melaleucus displays hemagglutination activity and antitumor activity in a b16 melanoma mouse model |
| topic | Phellodon melaleucus Lectin Hemagglutination activity Cytokine IL-10 Antitumor activity |
| url | http://www.sciencedirect.com/science/article/pii/S221345302300040X |
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