Assessment of Viral Limit of Detection in Spiked, Unassembled High-Throughput Sequencing Datasets

High-throughput sequencing (HTS) technology is applied to plant virus diagnosis using virus discovery pipelines, and direct detection of plant viruses from unassembled HTS is needed. The e-probe diagnostic nucleic acid assay Microbe Finder (EDNA-MiFi) is a pipeline for pathogen detection for unassem...

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Bibliographic Details
Main Authors: Lizbeth Peña-Zúñiga, Andres Espindola, Darren Hagen, Akhtar Ali, Francisco Ochoa-Corona
Format: Article
Language:English
Published: The American Phytopathological Society 2025-06-01
Series:PhytoFrontiers
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Online Access:https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-11-24-0121-FI
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