Comparison between Hyperspectral Imaging and Chemical Analysis of Polyphenol Oxidase Activity on Fresh-Cut Apple Slices
The assessment of the quality of fresh-cut apple slices is important for processing, storage, market value, and consumption. Determination of polyphenol oxidase activity (PPO) in apples is critical for controlling the quality of the final product (i.e., dried apples and juices). Hyperspectral imagin...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2020/7012525 |
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| author | Luna Shrestha Boris Kulig Roberto Moscetti Riccardo Massantini Elke Pawelzik Oliver Hensel Barbara Sturm |
| author_facet | Luna Shrestha Boris Kulig Roberto Moscetti Riccardo Massantini Elke Pawelzik Oliver Hensel Barbara Sturm |
| author_sort | Luna Shrestha |
| collection | DOAJ |
| description | The assessment of the quality of fresh-cut apple slices is important for processing, storage, market value, and consumption. Determination of polyphenol oxidase activity (PPO) in apples is critical for controlling the quality of the final product (i.e., dried apples and juices). Hyperspectral imaging (HSI) is a nondestructive, noncontact, and rapid food quality assessment technique. It has the potential to detect physical and chemical quality attributes of foods such as PPO of apple. The aim of this study was to investigate the suitability of HSI in the visible and near-infrared (VIS-NIR) range for indirect assessment of PPO activity of fresh-cut apple slices. Apple slices of two cultivars (cv. Golden Delicious and Elstar) were used to build a robust detection algorithm, which is independent of cultivars and applied treatments. Partial least squares (PLS) regression using the 7-fold cross-validation method and method comparison analysis (Bland–Altman plot, Passing–Bablok regression, and Deming regression) were performed. The 95% confidence interval (CI) bands for the Bland–Altman analysis between the methods were −4.19 and 13.11, and the mean difference was 3.7e−12. The Passing–Bablok regression had a slope of 0.8 and an intercept of 7.6. The slope of the Deming regression was 0.8 within the CI bands of 0.56 and 1.10. These results show acceptable performance and no significant deviation from linearity. Hence, the results demonstrated the feasibility of HSI as an indirect alternative to the standard chemical analysis of PPO enzyme activity. |
| format | Article |
| id | doaj-art-74f6d74510a54b04bf6aa20aa6d64fd2 |
| institution | Kabale University |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-74f6d74510a54b04bf6aa20aa6d64fd22025-02-03T06:46:31ZengWileyJournal of Spectroscopy2314-49202314-49392020-01-01202010.1155/2020/70125257012525Comparison between Hyperspectral Imaging and Chemical Analysis of Polyphenol Oxidase Activity on Fresh-Cut Apple SlicesLuna Shrestha0Boris Kulig1Roberto Moscetti2Riccardo Massantini3Elke Pawelzik4Oliver Hensel5Barbara Sturm6Department of Agricultural and Biosystems Engineering, University of Kassel, Witzenhausen, GermanyDepartment of Agricultural and Biosystems Engineering, University of Kassel, Witzenhausen, GermanyDepartment for Innovation in Biological, Agro-Food and Forest Systems (DIBAF), University of Tuscia, Viterbo, ItalyDepartment for Innovation in Biological, Agro-Food and Forest Systems (DIBAF), University of Tuscia, Viterbo, ItalyQuality of Plant Products Section, Department of Crop Sciences, Faculty of Agriculture, University of Göttingen, Göttingen, GermanyDepartment of Agricultural and Biosystems Engineering, University of Kassel, Witzenhausen, GermanyDepartment of Agricultural and Biosystems Engineering, University of Kassel, Witzenhausen, GermanyThe assessment of the quality of fresh-cut apple slices is important for processing, storage, market value, and consumption. Determination of polyphenol oxidase activity (PPO) in apples is critical for controlling the quality of the final product (i.e., dried apples and juices). Hyperspectral imaging (HSI) is a nondestructive, noncontact, and rapid food quality assessment technique. It has the potential to detect physical and chemical quality attributes of foods such as PPO of apple. The aim of this study was to investigate the suitability of HSI in the visible and near-infrared (VIS-NIR) range for indirect assessment of PPO activity of fresh-cut apple slices. Apple slices of two cultivars (cv. Golden Delicious and Elstar) were used to build a robust detection algorithm, which is independent of cultivars and applied treatments. Partial least squares (PLS) regression using the 7-fold cross-validation method and method comparison analysis (Bland–Altman plot, Passing–Bablok regression, and Deming regression) were performed. The 95% confidence interval (CI) bands for the Bland–Altman analysis between the methods were −4.19 and 13.11, and the mean difference was 3.7e−12. The Passing–Bablok regression had a slope of 0.8 and an intercept of 7.6. The slope of the Deming regression was 0.8 within the CI bands of 0.56 and 1.10. These results show acceptable performance and no significant deviation from linearity. Hence, the results demonstrated the feasibility of HSI as an indirect alternative to the standard chemical analysis of PPO enzyme activity.http://dx.doi.org/10.1155/2020/7012525 |
| spellingShingle | Luna Shrestha Boris Kulig Roberto Moscetti Riccardo Massantini Elke Pawelzik Oliver Hensel Barbara Sturm Comparison between Hyperspectral Imaging and Chemical Analysis of Polyphenol Oxidase Activity on Fresh-Cut Apple Slices Journal of Spectroscopy |
| title | Comparison between Hyperspectral Imaging and Chemical Analysis of Polyphenol Oxidase Activity on Fresh-Cut Apple Slices |
| title_full | Comparison between Hyperspectral Imaging and Chemical Analysis of Polyphenol Oxidase Activity on Fresh-Cut Apple Slices |
| title_fullStr | Comparison between Hyperspectral Imaging and Chemical Analysis of Polyphenol Oxidase Activity on Fresh-Cut Apple Slices |
| title_full_unstemmed | Comparison between Hyperspectral Imaging and Chemical Analysis of Polyphenol Oxidase Activity on Fresh-Cut Apple Slices |
| title_short | Comparison between Hyperspectral Imaging and Chemical Analysis of Polyphenol Oxidase Activity on Fresh-Cut Apple Slices |
| title_sort | comparison between hyperspectral imaging and chemical analysis of polyphenol oxidase activity on fresh cut apple slices |
| url | http://dx.doi.org/10.1155/2020/7012525 |
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