Analysis of the effect of ALA-PDT on macrophages in footpad model of mice infected with Fonsecaea monophora based on single-cell sequencing
Chromoblastomycosis (CBM) is a chronic neglected fungal disease that causes serious damage to the physical and mental health of patients. 5-Aminolevulinic acid photodynamic therapy (ALA-PDT) has garnered significant attention in the recent era for the treatment of CBM and has exhibited promising eff...
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| Format: | Article |
| Language: | English |
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De Gruyter
2025-01-01
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| Series: | Open Medicine |
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| Online Access: | https://doi.org/10.1515/med-2024-1132 |
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| author | Chen Wenyi Wu Xuelin Yaqoob Muhammad Danish Liu Kangxing Hu Yanqing Ke Xiuling Hu Yongxuan |
| author_facet | Chen Wenyi Wu Xuelin Yaqoob Muhammad Danish Liu Kangxing Hu Yanqing Ke Xiuling Hu Yongxuan |
| author_sort | Chen Wenyi |
| collection | DOAJ |
| description | Chromoblastomycosis (CBM) is a chronic neglected fungal disease that causes serious damage to the physical and mental health of patients. 5-Aminolevulinic acid photodynamic therapy (ALA-PDT) has garnered significant attention in the recent era for the treatment of CBM and has exhibited promising effects in several clinical case reports. We established a mice footpad infection model with Fonsecaea monophora and analyzed the impact of PDT treatment on the immune response of macrophages using single-cell sequencing. The results showed that infection of the mouse footpad skin with F. monophora results in an increase in inflammatory cells, primarily mononuclear-macrophages, with the activation of complement and enhancement of cell chemotaxis, leading to upregulation of anti-infection-related pathways. After ALA-PDT treatment, the number of inflammatory cells decreased, while macrophages upregulated the expression of antigen-recognition-related genes, enhancing phagocytosis and autophagy-related biological functions. |
| format | Article |
| id | doaj-art-741f35e9cb5c4081bd8a657c4ada26e4 |
| institution | Kabale University |
| issn | 2391-5463 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Medicine |
| spelling | doaj-art-741f35e9cb5c4081bd8a657c4ada26e42025-01-20T11:09:04ZengDe GruyterOpen Medicine2391-54632025-01-01201e00096119510.1515/med-2024-1132Analysis of the effect of ALA-PDT on macrophages in footpad model of mice infected with Fonsecaea monophora based on single-cell sequencingChen Wenyi0Wu Xuelin1Yaqoob Muhammad Danish2Liu Kangxing3Hu Yanqing4Ke Xiuling5Hu Yongxuan6Department of Dermatology and Venereology, The Third Affiliated Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Dermatology and Venereology, The Third Affiliated Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Dermatology and Venereology, The Third Affiliated Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Dermatology and Venereology, The Third Affiliated Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Dermatology and Venereology, The Third Affiliated Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Dermatology and Venereology, The Third Affiliated Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Dermatology and Venereology, The Third Affiliated Hospital, Southern Medical University, 183 West Zhongshan Road, Guangzhou, ChinaChromoblastomycosis (CBM) is a chronic neglected fungal disease that causes serious damage to the physical and mental health of patients. 5-Aminolevulinic acid photodynamic therapy (ALA-PDT) has garnered significant attention in the recent era for the treatment of CBM and has exhibited promising effects in several clinical case reports. We established a mice footpad infection model with Fonsecaea monophora and analyzed the impact of PDT treatment on the immune response of macrophages using single-cell sequencing. The results showed that infection of the mouse footpad skin with F. monophora results in an increase in inflammatory cells, primarily mononuclear-macrophages, with the activation of complement and enhancement of cell chemotaxis, leading to upregulation of anti-infection-related pathways. After ALA-PDT treatment, the number of inflammatory cells decreased, while macrophages upregulated the expression of antigen-recognition-related genes, enhancing phagocytosis and autophagy-related biological functions.https://doi.org/10.1515/med-2024-1132photodynamic therapychromoblastomycosisfonsecaea monophoramacrophagesingle-cell sequencing |
| spellingShingle | Chen Wenyi Wu Xuelin Yaqoob Muhammad Danish Liu Kangxing Hu Yanqing Ke Xiuling Hu Yongxuan Analysis of the effect of ALA-PDT on macrophages in footpad model of mice infected with Fonsecaea monophora based on single-cell sequencing Open Medicine photodynamic therapy chromoblastomycosis fonsecaea monophora macrophage single-cell sequencing |
| title | Analysis of the effect of ALA-PDT on macrophages in footpad model of mice infected with Fonsecaea monophora based on single-cell sequencing |
| title_full | Analysis of the effect of ALA-PDT on macrophages in footpad model of mice infected with Fonsecaea monophora based on single-cell sequencing |
| title_fullStr | Analysis of the effect of ALA-PDT on macrophages in footpad model of mice infected with Fonsecaea monophora based on single-cell sequencing |
| title_full_unstemmed | Analysis of the effect of ALA-PDT on macrophages in footpad model of mice infected with Fonsecaea monophora based on single-cell sequencing |
| title_short | Analysis of the effect of ALA-PDT on macrophages in footpad model of mice infected with Fonsecaea monophora based on single-cell sequencing |
| title_sort | analysis of the effect of ala pdt on macrophages in footpad model of mice infected with fonsecaea monophora based on single cell sequencing |
| topic | photodynamic therapy chromoblastomycosis fonsecaea monophora macrophage single-cell sequencing |
| url | https://doi.org/10.1515/med-2024-1132 |
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