Anti-phenolic glycolipid antibodies in Mycobacterium bovis infected cattle
Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB), causes significant financial losses in the agricultural industry. Additionally, M. bovis transmission from animals to humans can result in zoonotic TB, especially in low- and middle-income countries (LMICs), highlighting the need...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | One Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352771425000187 |
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| author | Zijie Zhou Anouk van Hooij J. Hessel M. van Dijk Nina Musch Louise Pierneef Hamza Khalid Kees Franken Thomas Holder Neil Watt Anita L. Michel Jeroen D.C. Codée Martin Vordermeier Paul L.A.M. Corstjens Elisabeth M.D.L. van der Heijden Jayne C. Hope Annemieke Geluk |
| author_facet | Zijie Zhou Anouk van Hooij J. Hessel M. van Dijk Nina Musch Louise Pierneef Hamza Khalid Kees Franken Thomas Holder Neil Watt Anita L. Michel Jeroen D.C. Codée Martin Vordermeier Paul L.A.M. Corstjens Elisabeth M.D.L. van der Heijden Jayne C. Hope Annemieke Geluk |
| author_sort | Zijie Zhou |
| collection | DOAJ |
| description | Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB), causes significant financial losses in the agricultural industry. Additionally, M. bovis transmission from animals to humans can result in zoonotic TB, especially in low- and middle-income countries (LMICs), highlighting the need to enhance One Health surveillance to mitigate this threat.Antibodies directed against a major mycobacterial cell wall component of M. leprae, phenolic glycolipid-I (PGL-I), have shown excellent performance in identifying M. leprae infection in humans and animals. In this study, we therefore investigated whether antibodies against M. bovis PGL similarly represent a useful biomarker for M. bovis infection in cattle.Comparing sera from naturally M. bovis-infected and the single intradermal comparative cervical tuberculin test (SICCT)-negative cattle, we assessed the potential of M. bovis PGL antibodies to identify this mycobacterial infection. Our results show that serum levels of anti-M. bovis PGL IgG and -IgM in M. bovis-infected cattle were significantly higher than in the SICCT-negative cattle. The sensitivity for anti-M. bovis PGL IgM in infected animals was, however, moderate (44.9 %) and the false-positive rate was 6.3 % in SICCT-negative cattle. Notably, vaccination with BCG- or heat-killed M. bovis did not affect serum levels of anti-M. bovis PGL IgM in cattle. Moreover, none of the 57 anti-M. bovis PGL-seropositive cattle tested positive in the anti-M. leprae PGL-I assay. This study shows for the first time that anti M. bovis PGL antibodies can be detected in infected cattle: anti-M. bovis PGL IgM is a highly specific, but moderately sensitive biomarker for M. bovis infection in cattle, showing potential for differentiate infected from vaccinated animals (DIVA). It could be a valuable component in a multi-biomarker approach for diagnosing bTB. |
| format | Article |
| id | doaj-art-73fa00d965bb43c09b8a5ad7382798c0 |
| institution | Kabale University |
| issn | 2352-7714 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | One Health |
| spelling | doaj-art-73fa00d965bb43c09b8a5ad7382798c02025-02-02T05:27:44ZengElsevierOne Health2352-77142025-06-0120100982Anti-phenolic glycolipid antibodies in Mycobacterium bovis infected cattleZijie Zhou0Anouk van Hooij1J. Hessel M. van Dijk2Nina Musch3Louise Pierneef4Hamza Khalid5Kees Franken6Thomas Holder7Neil Watt8Anita L. Michel9Jeroen D.C. Codée10Martin Vordermeier11Paul L.A.M. Corstjens12Elisabeth M.D.L. van der Heijden13Jayne C. Hope14Annemieke Geluk15Department of Infectious Diseases, Leiden University Medical Center, Leiden, the NetherlandsDepartment of Infectious Diseases, Leiden University Medical Center, Leiden, the NetherlandsDepartment of Bio-Organic Synthesis, Leiden Institute of Chemistry, Leiden University, Leiden, the NetherlandsDepartment of Infectious Diseases, Leiden University Medical Center, Leiden, the NetherlandsDepartment of Infectious Diseases, Leiden University Medical Center, Leiden, the NetherlandsDivision of Immunology, The Roslin Institute, University of Edinburgh, Easter Bush Campus, Midlothian, EH25 9RG, United KingdomDepartment of Infectious Diseases, Leiden University Medical Center, Leiden, the NetherlandsDepartment of Bacteriology, Animal and Plant Health Agency, Woodham, New Haw, KT15 3NB, United KingdomMV Diagnostics Ltd, Roslin Innovation Centre, University of Edinburgh, Easter Bush Campus, Midlothian, EHG25 9RG, United KingdomDepartment of Veterinary Tropical Disease, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South AfricaDepartment of Bio-Organic Synthesis, Leiden Institute of Chemistry, Leiden University, Leiden, the NetherlandsMV Diagnostics Ltd, Roslin Innovation Centre, University of Edinburgh, Easter Bush Campus, Midlothian, EHG25 9RG, United KingdomDepartment of Cell and Chemical Biology, Leiden University Medical Center, Leiden, the NetherlandsDepartment of Biomolecular Health Sciences, Division of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the NetherlandsDepartment of Bacteriology, Animal and Plant Health Agency, Woodham, New Haw, KT15 3NB, United KingdomDepartment of Infectious Diseases, Leiden University Medical Center, Leiden, the Netherlands; Corresponding author.Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB), causes significant financial losses in the agricultural industry. Additionally, M. bovis transmission from animals to humans can result in zoonotic TB, especially in low- and middle-income countries (LMICs), highlighting the need to enhance One Health surveillance to mitigate this threat.Antibodies directed against a major mycobacterial cell wall component of M. leprae, phenolic glycolipid-I (PGL-I), have shown excellent performance in identifying M. leprae infection in humans and animals. In this study, we therefore investigated whether antibodies against M. bovis PGL similarly represent a useful biomarker for M. bovis infection in cattle.Comparing sera from naturally M. bovis-infected and the single intradermal comparative cervical tuberculin test (SICCT)-negative cattle, we assessed the potential of M. bovis PGL antibodies to identify this mycobacterial infection. Our results show that serum levels of anti-M. bovis PGL IgG and -IgM in M. bovis-infected cattle were significantly higher than in the SICCT-negative cattle. The sensitivity for anti-M. bovis PGL IgM in infected animals was, however, moderate (44.9 %) and the false-positive rate was 6.3 % in SICCT-negative cattle. Notably, vaccination with BCG- or heat-killed M. bovis did not affect serum levels of anti-M. bovis PGL IgM in cattle. Moreover, none of the 57 anti-M. bovis PGL-seropositive cattle tested positive in the anti-M. leprae PGL-I assay. This study shows for the first time that anti M. bovis PGL antibodies can be detected in infected cattle: anti-M. bovis PGL IgM is a highly specific, but moderately sensitive biomarker for M. bovis infection in cattle, showing potential for differentiate infected from vaccinated animals (DIVA). It could be a valuable component in a multi-biomarker approach for diagnosing bTB.http://www.sciencedirect.com/science/article/pii/S2352771425000187AntibodieshhBovineDiagnosisDIVAM. bovisPhenolic glycolipid |
| spellingShingle | Zijie Zhou Anouk van Hooij J. Hessel M. van Dijk Nina Musch Louise Pierneef Hamza Khalid Kees Franken Thomas Holder Neil Watt Anita L. Michel Jeroen D.C. Codée Martin Vordermeier Paul L.A.M. Corstjens Elisabeth M.D.L. van der Heijden Jayne C. Hope Annemieke Geluk Anti-phenolic glycolipid antibodies in Mycobacterium bovis infected cattle One Health Antibodieshh Bovine Diagnosis DIVA M. bovis Phenolic glycolipid |
| title | Anti-phenolic glycolipid antibodies in Mycobacterium bovis infected cattle |
| title_full | Anti-phenolic glycolipid antibodies in Mycobacterium bovis infected cattle |
| title_fullStr | Anti-phenolic glycolipid antibodies in Mycobacterium bovis infected cattle |
| title_full_unstemmed | Anti-phenolic glycolipid antibodies in Mycobacterium bovis infected cattle |
| title_short | Anti-phenolic glycolipid antibodies in Mycobacterium bovis infected cattle |
| title_sort | anti phenolic glycolipid antibodies in mycobacterium bovis infected cattle |
| topic | Antibodieshh Bovine Diagnosis DIVA M. bovis Phenolic glycolipid |
| url | http://www.sciencedirect.com/science/article/pii/S2352771425000187 |
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