The Microbiota Determines Susceptibility to Experimental Autoimmune Uveoretinitis
The microbiota is a crucial modulator of the immune system. Here, we evaluated how its absence or reduction modifies the inflammatory response in the murine model of experimental autoimmune uveoretinitis (EAU). We induced EAU in germ-free (GF) or conventionally housed (CV) mice and in CV mice treate...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/5065703 |
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| author | Jarmila Heissigerova Petra Seidler Stangova Aneta Klimova Petra Svozilkova Tomas Hrncir Renata Stepankova Miloslav Kverka Helena Tlaskalova-Hogenova John V. Forrester |
| author_facet | Jarmila Heissigerova Petra Seidler Stangova Aneta Klimova Petra Svozilkova Tomas Hrncir Renata Stepankova Miloslav Kverka Helena Tlaskalova-Hogenova John V. Forrester |
| author_sort | Jarmila Heissigerova |
| collection | DOAJ |
| description | The microbiota is a crucial modulator of the immune system. Here, we evaluated how its absence or reduction modifies the inflammatory response in the murine model of experimental autoimmune uveoretinitis (EAU). We induced EAU in germ-free (GF) or conventionally housed (CV) mice and in CV mice treated with a combination of broad-spectrum antibiotics either from the day of EAU induction or from one week prior to induction of disease. The severity of the inflammation was assessed by fundus biomicroscopy or by histology, including immunohistology. The immunophenotyping of T cells in local and distant lymph nodes was performed by flow cytometry. We found that GF mice and mice where the microbiota was reduced one week before EAU induction were protected from severe autoimmune inflammation. GF mice had lower numbers of infiltrating macrophages and significantly less T cell infiltration in the retina than CV mice with EAU. GF mice also had reduced numbers of IFN-γ and IL-17-producing T cells and increased numbers of regulatory T cells in the eye-draining lymph nodes. These data suggest that the presence of microbiota during autoantigen recognition regulates the inflammatory response by influencing the adaptive immune response. |
| format | Article |
| id | doaj-art-734e4313087e4b6c9304c8b257acd57a |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-734e4313087e4b6c9304c8b257acd57a2025-02-03T01:10:14ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/50657035065703The Microbiota Determines Susceptibility to Experimental Autoimmune UveoretinitisJarmila Heissigerova0Petra Seidler Stangova1Aneta Klimova2Petra Svozilkova3Tomas Hrncir4Renata Stepankova5Miloslav Kverka6Helena Tlaskalova-Hogenova7John V. Forrester8Department of Ophthalmology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, U Nemocnice 2, 12808 Prague 2, Czech RepublicDepartment of Ophthalmology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, U Nemocnice 2, 12808 Prague 2, Czech RepublicDepartment of Ophthalmology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, U Nemocnice 2, 12808 Prague 2, Czech RepublicDepartment of Ophthalmology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, U Nemocnice 2, 12808 Prague 2, Czech RepublicInstitute of Microbiology of the Czech Academy of Sciences, v.v.i., Prague, Videnska 1083, 14220 Prague 4, Czech RepublicInstitute of Microbiology of the Czech Academy of Sciences, v.v.i., Prague, Videnska 1083, 14220 Prague 4, Czech RepublicInstitute of Microbiology of the Czech Academy of Sciences, v.v.i., Prague, Videnska 1083, 14220 Prague 4, Czech RepublicInstitute of Microbiology of the Czech Academy of Sciences, v.v.i., Prague, Videnska 1083, 14220 Prague 4, Czech RepublicSection of Immunology and Infection, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB252ZD, UKThe microbiota is a crucial modulator of the immune system. Here, we evaluated how its absence or reduction modifies the inflammatory response in the murine model of experimental autoimmune uveoretinitis (EAU). We induced EAU in germ-free (GF) or conventionally housed (CV) mice and in CV mice treated with a combination of broad-spectrum antibiotics either from the day of EAU induction or from one week prior to induction of disease. The severity of the inflammation was assessed by fundus biomicroscopy or by histology, including immunohistology. The immunophenotyping of T cells in local and distant lymph nodes was performed by flow cytometry. We found that GF mice and mice where the microbiota was reduced one week before EAU induction were protected from severe autoimmune inflammation. GF mice had lower numbers of infiltrating macrophages and significantly less T cell infiltration in the retina than CV mice with EAU. GF mice also had reduced numbers of IFN-γ and IL-17-producing T cells and increased numbers of regulatory T cells in the eye-draining lymph nodes. These data suggest that the presence of microbiota during autoantigen recognition regulates the inflammatory response by influencing the adaptive immune response.http://dx.doi.org/10.1155/2016/5065703 |
| spellingShingle | Jarmila Heissigerova Petra Seidler Stangova Aneta Klimova Petra Svozilkova Tomas Hrncir Renata Stepankova Miloslav Kverka Helena Tlaskalova-Hogenova John V. Forrester The Microbiota Determines Susceptibility to Experimental Autoimmune Uveoretinitis Journal of Immunology Research |
| title | The Microbiota Determines Susceptibility to Experimental Autoimmune Uveoretinitis |
| title_full | The Microbiota Determines Susceptibility to Experimental Autoimmune Uveoretinitis |
| title_fullStr | The Microbiota Determines Susceptibility to Experimental Autoimmune Uveoretinitis |
| title_full_unstemmed | The Microbiota Determines Susceptibility to Experimental Autoimmune Uveoretinitis |
| title_short | The Microbiota Determines Susceptibility to Experimental Autoimmune Uveoretinitis |
| title_sort | microbiota determines susceptibility to experimental autoimmune uveoretinitis |
| url | http://dx.doi.org/10.1155/2016/5065703 |
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