Use of ATR-FTIR Spectroscopy and Chemometrics for the Variation of Active Components in Different Harvesting Periods of Lonicera japonica
Lonicera japonica Thunb is a commonly used Chinese herbal medicine, which belongs to the family Caprifoliaceae. The active components varied greatly during bud development. Research on the variation of the main active components is significant for the timely harvesting and quality control of Lonicer...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2022/8850914 |
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| author | Yun-Chu Zhang Jing Deng Xiu-Lian Lin Ya-Mei Li Han-Xuan Sheng Bo-Hou Xia Li-Mei Lin |
| author_facet | Yun-Chu Zhang Jing Deng Xiu-Lian Lin Ya-Mei Li Han-Xuan Sheng Bo-Hou Xia Li-Mei Lin |
| author_sort | Yun-Chu Zhang |
| collection | DOAJ |
| description | Lonicera japonica Thunb is a commonly used Chinese herbal medicine, which belongs to the family Caprifoliaceae. The active components varied greatly during bud development. Research on the variation of the main active components is significant for the timely harvesting and quality control of Lonicera japonica. In this study, the attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) combined with the chemometric method was performed to investigate the variability of different harvesting periods of Lonicera japonica. The preliminary characterization from ATR-FTIR fingerprints showed various characteristic absorption peaks of the main active components from the different harvesting times, such as flavonoids, organic acids, iridoids, and volatile oils. Additionally, principal component analysis (PCA) scatter plots showed that there was a clear clustering trend in the samples of the same harvesting period, and the samples of the different harvesting periods could be well distinguished. Finally, further analysis by the orthogonal partial least-squares discriminant analysis (OPLS-DA) showed that there were regular changes in flavonoids, phenolic acids, iridoids, and volatile oils in different harvesting periods. Therefore, ATR-FTIR, as a novel and convenient analytical method, could be applied to evaluate the quality of Lonicera japonica. |
| format | Article |
| id | doaj-art-72e0f36cf22a405cb35bd9ba24c952ec |
| institution | Kabale University |
| issn | 1687-8779 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-72e0f36cf22a405cb35bd9ba24c952ec2025-02-03T01:11:58ZengWileyInternational Journal of Analytical Chemistry1687-87792022-01-01202210.1155/2022/8850914Use of ATR-FTIR Spectroscopy and Chemometrics for the Variation of Active Components in Different Harvesting Periods of Lonicera japonicaYun-Chu Zhang0Jing Deng1Xiu-Lian Lin2Ya-Mei Li3Han-Xuan Sheng4Bo-Hou Xia5Li-Mei Lin6Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan ProvinceKey Laboratory for Quality Evaluation of Bulk Herbs of Hunan ProvinceKey Laboratory for Quality Evaluation of Bulk Herbs of Hunan ProvinceKey Laboratory for Quality Evaluation of Bulk Herbs of Hunan ProvinceKey Laboratory for Quality Evaluation of Bulk Herbs of Hunan ProvinceKey Laboratory for Quality Evaluation of Bulk Herbs of Hunan ProvinceKey Laboratory for Quality Evaluation of Bulk Herbs of Hunan ProvinceLonicera japonica Thunb is a commonly used Chinese herbal medicine, which belongs to the family Caprifoliaceae. The active components varied greatly during bud development. Research on the variation of the main active components is significant for the timely harvesting and quality control of Lonicera japonica. In this study, the attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) combined with the chemometric method was performed to investigate the variability of different harvesting periods of Lonicera japonica. The preliminary characterization from ATR-FTIR fingerprints showed various characteristic absorption peaks of the main active components from the different harvesting times, such as flavonoids, organic acids, iridoids, and volatile oils. Additionally, principal component analysis (PCA) scatter plots showed that there was a clear clustering trend in the samples of the same harvesting period, and the samples of the different harvesting periods could be well distinguished. Finally, further analysis by the orthogonal partial least-squares discriminant analysis (OPLS-DA) showed that there were regular changes in flavonoids, phenolic acids, iridoids, and volatile oils in different harvesting periods. Therefore, ATR-FTIR, as a novel and convenient analytical method, could be applied to evaluate the quality of Lonicera japonica.http://dx.doi.org/10.1155/2022/8850914 |
| spellingShingle | Yun-Chu Zhang Jing Deng Xiu-Lian Lin Ya-Mei Li Han-Xuan Sheng Bo-Hou Xia Li-Mei Lin Use of ATR-FTIR Spectroscopy and Chemometrics for the Variation of Active Components in Different Harvesting Periods of Lonicera japonica International Journal of Analytical Chemistry |
| title | Use of ATR-FTIR Spectroscopy and Chemometrics for the Variation of Active Components in Different Harvesting Periods of Lonicera japonica |
| title_full | Use of ATR-FTIR Spectroscopy and Chemometrics for the Variation of Active Components in Different Harvesting Periods of Lonicera japonica |
| title_fullStr | Use of ATR-FTIR Spectroscopy and Chemometrics for the Variation of Active Components in Different Harvesting Periods of Lonicera japonica |
| title_full_unstemmed | Use of ATR-FTIR Spectroscopy and Chemometrics for the Variation of Active Components in Different Harvesting Periods of Lonicera japonica |
| title_short | Use of ATR-FTIR Spectroscopy and Chemometrics for the Variation of Active Components in Different Harvesting Periods of Lonicera japonica |
| title_sort | use of atr ftir spectroscopy and chemometrics for the variation of active components in different harvesting periods of lonicera japonica |
| url | http://dx.doi.org/10.1155/2022/8850914 |
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