High contrast fluorescence polarization microscopy through double tagged photoswitchable fluorescent proteins
Abstract We demonstrate that rigid anchoring of fluorescent proteins through double tagging (FPs) in living cells can significantly enhance the contrast in fluorescence polarization microscopy (FPM) by locking the transition dipole moment orientations to the sample’s structures. We applied double ta...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-07-01
|
| Series: | npj Imaging |
| Online Access: | https://doi.org/10.1038/s44303-025-00094-y |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|