Uptake of Photosensitizer 2-Devinyl-2-(1-methoxylethyl) Chlorin f in Human Breast Cancer Cells: A Diffusion Kinetics Study
The kinetics of photosensitizer 2-devinyl-2-(1-methoxylethyl) chlorin f (CPD4) uptake in MCF-7 human breast cancer cells is described by a diffusion kinetics model and experimentally investigated using laser scanning confocal microscopy (LSCM). CPD4 permeated into MCF-7 cells with increasing incubat...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Photoenergy |
| Online Access: | http://dx.doi.org/10.1155/2012/192380 |
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| _version_ | 1832549307511734272 |
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| author | Ping Chen Feng Zhang Lei Zhang Song-Cheng Mao Lie Lin Hua Bai Guo-Qing Tang Jian-Zhong Yao Jun-Hong Yang Yun-Feng Ma |
| author_facet | Ping Chen Feng Zhang Lei Zhang Song-Cheng Mao Lie Lin Hua Bai Guo-Qing Tang Jian-Zhong Yao Jun-Hong Yang Yun-Feng Ma |
| author_sort | Ping Chen |
| collection | DOAJ |
| description | The kinetics of photosensitizer 2-devinyl-2-(1-methoxylethyl) chlorin f (CPD4) uptake in MCF-7 human breast cancer cells is described by a diffusion kinetics model and experimentally investigated using laser scanning confocal microscopy (LSCM). CPD4 permeated into MCF-7 cells with increasing incubation time, which was followed by its binding to cell organelles. Subcellular distribution study revealed that CPD4 was primarily localized on the mitochondria and membranes, supporting that the mode of transmembrane transport was diffusion. A kinetics model describing CPD4 passing through the plasma membrane of MCF-7 cells was proposed based on Fick's first law of diffusion. The kinetics of cellular uptake of CPD4 was studied by three-dimensional LSCM. By fitting the experimental data using the above model, important cellular uptake and distribution parameters were obtained, which are of clinical significance in photodynamic therapy. |
| format | Article |
| id | doaj-art-72b0e78d30c84d0eae40a165d969752e |
| institution | Kabale University |
| issn | 1110-662X 1687-529X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Photoenergy |
| spelling | doaj-art-72b0e78d30c84d0eae40a165d969752e2025-02-03T06:11:36ZengWileyInternational Journal of Photoenergy1110-662X1687-529X2012-01-01201210.1155/2012/192380192380Uptake of Photosensitizer 2-Devinyl-2-(1-methoxylethyl) Chlorin f in Human Breast Cancer Cells: A Diffusion Kinetics StudyPing Chen0Feng Zhang1Lei Zhang2Song-Cheng Mao3Lie Lin4Hua Bai5Guo-Qing Tang6Jian-Zhong Yao7Jun-Hong Yang8Yun-Feng Ma9Institute of Modern Optics, Nankai University, Tianjin 300071, ChinaInstitute of Modern Optics, Nankai University, Tianjin 300071, ChinaInstitute of Modern Optics, Nankai University, Tianjin 300071, ChinaInstitute of Modern Optics, Nankai University, Tianjin 300071, ChinaInstitute of Modern Optics, Nankai University, Tianjin 300071, ChinaInstitute of Modern Optics, Nankai University, Tianjin 300071, ChinaInstitute of Modern Optics, Nankai University, Tianjin 300071, ChinaSchool of Pharmacy, Second Military Medical University, Shanghai 200433, ChinaR&D Department, Beijing GK Laser Technology Co., Ltd., No. 66 Xixiaokou Road, Beijing 100192, ChinaR&D Department, Beijing GK Laser Technology Co., Ltd., No. 66 Xixiaokou Road, Beijing 100192, ChinaThe kinetics of photosensitizer 2-devinyl-2-(1-methoxylethyl) chlorin f (CPD4) uptake in MCF-7 human breast cancer cells is described by a diffusion kinetics model and experimentally investigated using laser scanning confocal microscopy (LSCM). CPD4 permeated into MCF-7 cells with increasing incubation time, which was followed by its binding to cell organelles. Subcellular distribution study revealed that CPD4 was primarily localized on the mitochondria and membranes, supporting that the mode of transmembrane transport was diffusion. A kinetics model describing CPD4 passing through the plasma membrane of MCF-7 cells was proposed based on Fick's first law of diffusion. The kinetics of cellular uptake of CPD4 was studied by three-dimensional LSCM. By fitting the experimental data using the above model, important cellular uptake and distribution parameters were obtained, which are of clinical significance in photodynamic therapy.http://dx.doi.org/10.1155/2012/192380 |
| spellingShingle | Ping Chen Feng Zhang Lei Zhang Song-Cheng Mao Lie Lin Hua Bai Guo-Qing Tang Jian-Zhong Yao Jun-Hong Yang Yun-Feng Ma Uptake of Photosensitizer 2-Devinyl-2-(1-methoxylethyl) Chlorin f in Human Breast Cancer Cells: A Diffusion Kinetics Study International Journal of Photoenergy |
| title | Uptake of Photosensitizer 2-Devinyl-2-(1-methoxylethyl) Chlorin f in Human Breast Cancer Cells: A Diffusion Kinetics Study |
| title_full | Uptake of Photosensitizer 2-Devinyl-2-(1-methoxylethyl) Chlorin f in Human Breast Cancer Cells: A Diffusion Kinetics Study |
| title_fullStr | Uptake of Photosensitizer 2-Devinyl-2-(1-methoxylethyl) Chlorin f in Human Breast Cancer Cells: A Diffusion Kinetics Study |
| title_full_unstemmed | Uptake of Photosensitizer 2-Devinyl-2-(1-methoxylethyl) Chlorin f in Human Breast Cancer Cells: A Diffusion Kinetics Study |
| title_short | Uptake of Photosensitizer 2-Devinyl-2-(1-methoxylethyl) Chlorin f in Human Breast Cancer Cells: A Diffusion Kinetics Study |
| title_sort | uptake of photosensitizer 2 devinyl 2 1 methoxylethyl chlorin f in human breast cancer cells a diffusion kinetics study |
| url | http://dx.doi.org/10.1155/2012/192380 |
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