Solid-Phase Extraction of Pesticides by Using Bioinspired Peptide Receptors
A virtual development of hexapeptide receptors bioinspired by the acetylcholinesterase enzyme active site is proposed. A semicombinatorial approach was applied to generate a virtual hexapeptides library with different affinity properties towards organophosphate and carbamate pesticides. The virtual...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/905701 |
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| author | Valentina Lanzone Manuel Sergi Marcello Mascini Rossana Scarpone Flavio Della Pelle Michele Del Carlo Giampiero Scortichini Dario Compagnone |
| author_facet | Valentina Lanzone Manuel Sergi Marcello Mascini Rossana Scarpone Flavio Della Pelle Michele Del Carlo Giampiero Scortichini Dario Compagnone |
| author_sort | Valentina Lanzone |
| collection | DOAJ |
| description | A virtual development of hexapeptide receptors bioinspired by the acetylcholinesterase enzyme active site is proposed. A semicombinatorial approach was applied to generate a virtual hexapeptides library with different affinity properties towards organophosphate and carbamate pesticides. The virtual screening process was addressed to obtain peptides able to separate pesticide subclasses in the experimental work. Three hexapeptides, two generated by molecular modeling and one having a scrambled sequence, were used as selective sorbent materials for pesticides in preanalytical solid-phase extraction (SPE) method. Selective adsorption and cross-reactivity were tested directly on a mix of four pesticides (carbaryl, chlorpyrifos-ethyl, malathion, and thiabendazole) having different structures and physico-chemical properties, at a total concentration of 120 ppb (each pesticide at concentration of 30 ppb). The results were compared to traditional sorbent material such as C-18 and strata-X. Data showed that only one of the hexapeptides virtually designed had significant differences in competitive absorption between aliphatic pesticide malathion, fungicide thiabendazole chosen as negative control, and aromatic pesticides. These results partially supported the simulated strategy. |
| format | Article |
| id | doaj-art-726cde6d3ad146349dfaead2fedba535 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-726cde6d3ad146349dfaead2fedba5352025-02-03T01:21:00ZengWileyJournal of Chemistry2090-90632090-90712015-01-01201510.1155/2015/905701905701Solid-Phase Extraction of Pesticides by Using Bioinspired Peptide ReceptorsValentina Lanzone0Manuel Sergi1Marcello Mascini2Rossana Scarpone3Flavio Della Pelle4Michele Del Carlo5Giampiero Scortichini6Dario Compagnone7Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64023 Teramo, ItalyFaculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64023 Teramo, ItalyFaculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64023 Teramo, ItalyIstituto Zooprofilattico dell’Abruzzo e del Molise “G. Caporale”, 64100 Teramo, ItalyFaculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64023 Teramo, ItalyFaculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64023 Teramo, ItalyIstituto Zooprofilattico dell’Abruzzo e del Molise “G. Caporale”, 64100 Teramo, ItalyFaculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64023 Teramo, ItalyA virtual development of hexapeptide receptors bioinspired by the acetylcholinesterase enzyme active site is proposed. A semicombinatorial approach was applied to generate a virtual hexapeptides library with different affinity properties towards organophosphate and carbamate pesticides. The virtual screening process was addressed to obtain peptides able to separate pesticide subclasses in the experimental work. Three hexapeptides, two generated by molecular modeling and one having a scrambled sequence, were used as selective sorbent materials for pesticides in preanalytical solid-phase extraction (SPE) method. Selective adsorption and cross-reactivity were tested directly on a mix of four pesticides (carbaryl, chlorpyrifos-ethyl, malathion, and thiabendazole) having different structures and physico-chemical properties, at a total concentration of 120 ppb (each pesticide at concentration of 30 ppb). The results were compared to traditional sorbent material such as C-18 and strata-X. Data showed that only one of the hexapeptides virtually designed had significant differences in competitive absorption between aliphatic pesticide malathion, fungicide thiabendazole chosen as negative control, and aromatic pesticides. These results partially supported the simulated strategy.http://dx.doi.org/10.1155/2015/905701 |
| spellingShingle | Valentina Lanzone Manuel Sergi Marcello Mascini Rossana Scarpone Flavio Della Pelle Michele Del Carlo Giampiero Scortichini Dario Compagnone Solid-Phase Extraction of Pesticides by Using Bioinspired Peptide Receptors Journal of Chemistry |
| title | Solid-Phase Extraction of Pesticides by Using Bioinspired Peptide Receptors |
| title_full | Solid-Phase Extraction of Pesticides by Using Bioinspired Peptide Receptors |
| title_fullStr | Solid-Phase Extraction of Pesticides by Using Bioinspired Peptide Receptors |
| title_full_unstemmed | Solid-Phase Extraction of Pesticides by Using Bioinspired Peptide Receptors |
| title_short | Solid-Phase Extraction of Pesticides by Using Bioinspired Peptide Receptors |
| title_sort | solid phase extraction of pesticides by using bioinspired peptide receptors |
| url | http://dx.doi.org/10.1155/2015/905701 |
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