High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents
Abstract Cryoprotective agent (CPA) toxicity is the most limiting factor impeding cryopreservation of critically needed tissues and organs for transplantation and medical research. This limitation is in part due to the challenge of rapidly screening compounds to identify candidate molecules that are...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-85509-x |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832594768980344832 |
|---|---|
| author | Nima Ahmadkhani James D. Benson Ali Eroglu Adam Z. Higgins |
| author_facet | Nima Ahmadkhani James D. Benson Ali Eroglu Adam Z. Higgins |
| author_sort | Nima Ahmadkhani |
| collection | DOAJ |
| description | Abstract Cryoprotective agent (CPA) toxicity is the most limiting factor impeding cryopreservation of critically needed tissues and organs for transplantation and medical research. This limitation is in part due to the challenge of rapidly screening compounds to identify candidate molecules that are highly membrane permeable and non-toxic at high concentrations. Such a combination would facilitate rapid CPA permeation throughout the sample, enabling ice-free cryopreservation with minimal toxicity. This study presents a new method for rapidly assessing the cell membrane permeability and toxicity of candidate CPAs. The new method enables ~ 100 times faster permeability measurement than previous methods, while also allowing assessment of CPA toxicity using the same 96-well plate. We tested five commonly used CPAs and 22 less common ones at both 4 °C and room temperature, with 23 of them passing the screening process based on their favorable toxicity and permeability properties. Considering its advantages such as high throughput measurement of membrane permeability along with simultaneous toxicity assessment, the presented method holds promise as an effective initial screening tool to identify new CPAs for cryopreservation. |
| format | Article |
| id | doaj-art-71a9e9c9e83e44c6b87cb3ab040e6726 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-71a9e9c9e83e44c6b87cb3ab040e67262025-01-19T12:21:10ZengNature PortfolioScientific Reports2045-23222025-01-0115111310.1038/s41598-025-85509-xHigh throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agentsNima Ahmadkhani0James D. Benson1Ali Eroglu2Adam Z. Higgins3School of Chemical, Biological, and Environmental Engineering, Oregon State UniversityDepartment of Biology, University of SaskatchewanDepartment of Neuroscience and Regenerative Medicine, Medical College of Georgia - Augusta UniversitySchool of Chemical, Biological, and Environmental Engineering, Oregon State UniversityAbstract Cryoprotective agent (CPA) toxicity is the most limiting factor impeding cryopreservation of critically needed tissues and organs for transplantation and medical research. This limitation is in part due to the challenge of rapidly screening compounds to identify candidate molecules that are highly membrane permeable and non-toxic at high concentrations. Such a combination would facilitate rapid CPA permeation throughout the sample, enabling ice-free cryopreservation with minimal toxicity. This study presents a new method for rapidly assessing the cell membrane permeability and toxicity of candidate CPAs. The new method enables ~ 100 times faster permeability measurement than previous methods, while also allowing assessment of CPA toxicity using the same 96-well plate. We tested five commonly used CPAs and 22 less common ones at both 4 °C and room temperature, with 23 of them passing the screening process based on their favorable toxicity and permeability properties. Considering its advantages such as high throughput measurement of membrane permeability along with simultaneous toxicity assessment, the presented method holds promise as an effective initial screening tool to identify new CPAs for cryopreservation.https://doi.org/10.1038/s41598-025-85509-xCryopreservationVitrificationFreezingCell membrane permeabilityCPACryoprotectant |
| spellingShingle | Nima Ahmadkhani James D. Benson Ali Eroglu Adam Z. Higgins High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents Scientific Reports Cryopreservation Vitrification Freezing Cell membrane permeability CPA Cryoprotectant |
| title | High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents |
| title_full | High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents |
| title_fullStr | High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents |
| title_full_unstemmed | High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents |
| title_short | High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents |
| title_sort | high throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents |
| topic | Cryopreservation Vitrification Freezing Cell membrane permeability CPA Cryoprotectant |
| url | https://doi.org/10.1038/s41598-025-85509-x |
| work_keys_str_mv | AT nimaahmadkhani highthroughputmethodforsimultaneousscreeningofmembranepermeabilityandtoxicityfordiscoveryofnewcryoprotectiveagents AT jamesdbenson highthroughputmethodforsimultaneousscreeningofmembranepermeabilityandtoxicityfordiscoveryofnewcryoprotectiveagents AT alieroglu highthroughputmethodforsimultaneousscreeningofmembranepermeabilityandtoxicityfordiscoveryofnewcryoprotectiveagents AT adamzhiggins highthroughputmethodforsimultaneousscreeningofmembranepermeabilityandtoxicityfordiscoveryofnewcryoprotectiveagents |