Fu Tu Sheng Jin Rehabilitation Formula Mitigate Airway Inflammation, Mucus Secretion and Immune Dysfunction Induced by SARS-CoV-2 Spike Protein
Bo-Han Wang,1,* Ke-Yao Yu,2,* Xiao-Na Zhang,3,* Xian-Hong Sun,2 Ling-Ling Tang,4 Xiao-Lu Shi2 1NanJing JiangNing Hospital of Chinese Medicine/Affiliated jiangning Hospital of Chinese Medicine, China Pharmaceutical University, Nanjing, Jiangsu, 210029, People’s Republic of Chi...
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Dove Medical Press
2025-01-01
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| Series: | Journal of Inflammation Research |
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| author | Wang BH Yu KY Zhang XN Sun XH Tang LL Shi XL |
| author_facet | Wang BH Yu KY Zhang XN Sun XH Tang LL Shi XL |
| author_sort | Wang BH |
| collection | DOAJ |
| description | Bo-Han Wang,1,* Ke-Yao Yu,2,* Xiao-Na Zhang,3,* Xian-Hong Sun,2 Ling-Ling Tang,4 Xiao-Lu Shi2 1NanJing JiangNing Hospital of Chinese Medicine/Affiliated jiangning Hospital of Chinese Medicine, China Pharmaceutical University, Nanjing, Jiangsu, 210029, People’s Republic of China; 2Affiliated Hospital of Nanjing University of Chinese Medicine/ Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu, 210029, People’s Republic of China; 3Nanjing Hospital of Chinese Medicine, Nanjing, Jiangsu, 210029, People’s Republic of China; 4School of Chinese Medicine & School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210023, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiao-Lu Shi; Ling-Ling Tang, Email fsyy00721@njucm.edu.cn; 290602@njucm.edu.cnObjective: To evaluate the effects of Fu Tu Sheng Jin Rehabilitation Formula (FTSJRF) on airway inflammation, mucus secretion, and immunoreaction in a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein-induced mouse model.Methods: Forty-two mice were randomly divided into seven groups: normal, D1, D3, D10, D10H, D10M and D10L, according to the days of modeling and different dosages of FTSJRF. D1, D3, D10, D10H, D10M and D10L group mice were intratracheally administered with 15 μg SARS-CoV-2 spike protein; mice in the D10H, D10M, and D10L groups were intragastrically administered FTSJRF (46, 23 and 11.5 g/kg, respectively). Observe the pathological changes in lung tissues, expression of inflammatory factors, and mucins in different groups of mice using HE and PAS staining methods, as well as ELISA and RT-qPCR. Flow cytometry was used to detect T helper 17 (Th17)/regulatory T (Treg) cells and T helper 1(Th1)/T helper 2 (Th2) lymphocyte ratios and the proportions of conventional myeloid dendritic cells (cDCs), plasma cell-like DCs, CD80 and CD86 cells in mouse spleens.Results: HE and PAS staining showed that, compared to that in the normal group, the lung tissue of the D1 group mice showed a significant inflammatory damage response, whereas the D3 and D10 groups showed a gradual recovery trend. Groups D1 and D3 showed mild mucus secretion, whereas the D10 group had excessive mucus secretion. The D10 group of mice displayed increased levels of IL-4, TNF-α, IL-33 and mucin genes such as MUC1, MUC4, etc, and FTSJRF inhibited the expression of these molecules, mucus secretion and lung damage in SARS-CoV-2 spike protein-induced mouse model. Flow cytometry results showed a decrease in the number of cDCs and an abnormal recovery of DC mature cells in the D10 group. FTSJRF increased the number of cDCs and promoted DC maturation. A higher Th17/Treg ratio was observed in the D3 and D10 groups than in the normal group, whereas this ratio decreases under the effect of FTSJRF. D10 had significantly lower Th1/Th2 ratio than normal, D1 and D3 groups, and high doses of FTSJRF increased it.Conclusion: FTSJRF mitigates airway inflammation and mucus secretion induced by SARS-CoV-2 spike protein. Additionally, FTSJRF regulates immune functions by promoting DC maturation and Th17/Treg and Th1/Th2 cell homeostasis.Keywords: COVID-19, SARS-CoV-2 spike protein, Fu Tu ShengJin Rehabilitation Formula, airway inflammation, mucus secretion, immune dysfunction |
| format | Article |
| id | doaj-art-717f59785cf14865a838bbf25ee952e5 |
| institution | Kabale University |
| issn | 1178-7031 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Inflammation Research |
| spelling | doaj-art-717f59785cf14865a838bbf25ee952e52025-01-23T18:50:32ZengDove Medical PressJournal of Inflammation Research1178-70312025-01-01Volume 181053106599495Fu Tu Sheng Jin Rehabilitation Formula Mitigate Airway Inflammation, Mucus Secretion and Immune Dysfunction Induced by SARS-CoV-2 Spike ProteinWang BHYu KYZhang XNSun XHTang LLShi XLBo-Han Wang,1,* Ke-Yao Yu,2,* Xiao-Na Zhang,3,* Xian-Hong Sun,2 Ling-Ling Tang,4 Xiao-Lu Shi2 1NanJing JiangNing Hospital of Chinese Medicine/Affiliated jiangning Hospital of Chinese Medicine, China Pharmaceutical University, Nanjing, Jiangsu, 210029, People’s Republic of China; 2Affiliated Hospital of Nanjing University of Chinese Medicine/ Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu, 210029, People’s Republic of China; 3Nanjing Hospital of Chinese Medicine, Nanjing, Jiangsu, 210029, People’s Republic of China; 4School of Chinese Medicine & School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210023, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiao-Lu Shi; Ling-Ling Tang, Email fsyy00721@njucm.edu.cn; 290602@njucm.edu.cnObjective: To evaluate the effects of Fu Tu Sheng Jin Rehabilitation Formula (FTSJRF) on airway inflammation, mucus secretion, and immunoreaction in a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein-induced mouse model.Methods: Forty-two mice were randomly divided into seven groups: normal, D1, D3, D10, D10H, D10M and D10L, according to the days of modeling and different dosages of FTSJRF. D1, D3, D10, D10H, D10M and D10L group mice were intratracheally administered with 15 μg SARS-CoV-2 spike protein; mice in the D10H, D10M, and D10L groups were intragastrically administered FTSJRF (46, 23 and 11.5 g/kg, respectively). Observe the pathological changes in lung tissues, expression of inflammatory factors, and mucins in different groups of mice using HE and PAS staining methods, as well as ELISA and RT-qPCR. Flow cytometry was used to detect T helper 17 (Th17)/regulatory T (Treg) cells and T helper 1(Th1)/T helper 2 (Th2) lymphocyte ratios and the proportions of conventional myeloid dendritic cells (cDCs), plasma cell-like DCs, CD80 and CD86 cells in mouse spleens.Results: HE and PAS staining showed that, compared to that in the normal group, the lung tissue of the D1 group mice showed a significant inflammatory damage response, whereas the D3 and D10 groups showed a gradual recovery trend. Groups D1 and D3 showed mild mucus secretion, whereas the D10 group had excessive mucus secretion. The D10 group of mice displayed increased levels of IL-4, TNF-α, IL-33 and mucin genes such as MUC1, MUC4, etc, and FTSJRF inhibited the expression of these molecules, mucus secretion and lung damage in SARS-CoV-2 spike protein-induced mouse model. Flow cytometry results showed a decrease in the number of cDCs and an abnormal recovery of DC mature cells in the D10 group. FTSJRF increased the number of cDCs and promoted DC maturation. A higher Th17/Treg ratio was observed in the D3 and D10 groups than in the normal group, whereas this ratio decreases under the effect of FTSJRF. D10 had significantly lower Th1/Th2 ratio than normal, D1 and D3 groups, and high doses of FTSJRF increased it.Conclusion: FTSJRF mitigates airway inflammation and mucus secretion induced by SARS-CoV-2 spike protein. Additionally, FTSJRF regulates immune functions by promoting DC maturation and Th17/Treg and Th1/Th2 cell homeostasis.Keywords: COVID-19, SARS-CoV-2 spike protein, Fu Tu ShengJin Rehabilitation Formula, airway inflammation, mucus secretion, immune dysfunctionhttps://www.dovepress.com/fu-tu-sheng-jin-rehabilitation-formula-mitigate-airway-inflammation-mu-peer-reviewed-fulltext-article-JIRcovid-19sars-cov-2 spike proteinfu tu sheng jin rehabilitation formulaairway inflammationmucus secretionimmune dysfunction. |
| spellingShingle | Wang BH Yu KY Zhang XN Sun XH Tang LL Shi XL Fu Tu Sheng Jin Rehabilitation Formula Mitigate Airway Inflammation, Mucus Secretion and Immune Dysfunction Induced by SARS-CoV-2 Spike Protein Journal of Inflammation Research covid-19 sars-cov-2 spike protein fu tu sheng jin rehabilitation formula airway inflammation mucus secretion immune dysfunction. |
| title | Fu Tu Sheng Jin Rehabilitation Formula Mitigate Airway Inflammation, Mucus Secretion and Immune Dysfunction Induced by SARS-CoV-2 Spike Protein |
| title_full | Fu Tu Sheng Jin Rehabilitation Formula Mitigate Airway Inflammation, Mucus Secretion and Immune Dysfunction Induced by SARS-CoV-2 Spike Protein |
| title_fullStr | Fu Tu Sheng Jin Rehabilitation Formula Mitigate Airway Inflammation, Mucus Secretion and Immune Dysfunction Induced by SARS-CoV-2 Spike Protein |
| title_full_unstemmed | Fu Tu Sheng Jin Rehabilitation Formula Mitigate Airway Inflammation, Mucus Secretion and Immune Dysfunction Induced by SARS-CoV-2 Spike Protein |
| title_short | Fu Tu Sheng Jin Rehabilitation Formula Mitigate Airway Inflammation, Mucus Secretion and Immune Dysfunction Induced by SARS-CoV-2 Spike Protein |
| title_sort | fu tu sheng jin rehabilitation formula mitigate airway inflammation mucus secretion and immune dysfunction induced by sars cov 2 spike protein |
| topic | covid-19 sars-cov-2 spike protein fu tu sheng jin rehabilitation formula airway inflammation mucus secretion immune dysfunction. |
| url | https://www.dovepress.com/fu-tu-sheng-jin-rehabilitation-formula-mitigate-airway-inflammation-mu-peer-reviewed-fulltext-article-JIR |
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