Improved production strain maintenance technique for Burkholderia mallei 5584 (Master seed) used for glander diagnostic agent production
One of the aspects important for strain collection maintenance is the optimization of existing methods and development of new techniques for microbial strain preservation, that is why the improvement of previously developed methods for authentic strain preservation is an urgent task. The article pro...
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| Format: | Article |
| Language: | English |
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Da Vinci Media
2024-03-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/796 |
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| author | E. A. Artemeva L. A. Melnikova L. I. Zaynullin N. K. Bukova |
| author_facet | E. A. Artemeva L. A. Melnikova L. I. Zaynullin N. K. Bukova |
| author_sort | E. A. Artemeva |
| collection | DOAJ |
| description | One of the aspects important for strain collection maintenance is the optimization of existing methods and development of new techniques for microbial strain preservation, that is why the improvement of previously developed methods for authentic strain preservation is an urgent task. The article provides information on the maintenance of Burkholderia mallei 5584 (Master seed) using previously developed technique, which was supplemented with new stages in accordance with modern requirements for strain collections of highly dangerous disease agents. The previous strain maintenance technique involved its storage in its native state, which facilitated accumulation of genetic mutations and, ultimately modification of bacterial cell properties. To extend the storage time of this strain and to ensure the stability of its biological properties, the freeze-drying method was used. Skimmed milk was used as a cryoprotectant. Freeze-drying was performed under selected conditions. This technique allows for the strain sub-culturing on sensitive models once every 5 years, which is more expedient and safe from an economic and biological point of view. For safe handling of Burkholderia mallei 5584 production strain, an inactivation technique using gamma rays at 30 kGy was developed, which allowed to achieve microbial suspension sterility and preserve the bacterial cell structure. When comparing the previously developed and supplemented techniques, it was found that the improved technique of Burkholderia mallei 5584 (Master seed) maintenance makes it possible to avoid the loss of its biological properties needed for the production of high-quality laboratory diagnostic agents used for timely disease detection in susceptible animals by diagnostic tests. |
| format | Article |
| id | doaj-art-6feb09a513e74ea1808d809dcaa6dc24 |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-6feb09a513e74ea1808d809dcaa6dc242025-02-06T09:52:10ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592024-03-011319510210.29326/2304-196X-2024-13-1-95-102657Improved production strain maintenance technique for Burkholderia mallei 5584 (Master seed) used for glander diagnostic agent productionE. A. Artemeva0L. A. Melnikova1L. I. Zaynullin2N. K. Bukova3Federal Center for Toxicological, Radiation, and Biological SafetyFederal Center for Toxicological, Radiation, and Biological SafetyFederal Center for Toxicological, Radiation, and Biological SafetyThe Russian State Center for Animal Feed and Drug Standardization and QualityOne of the aspects important for strain collection maintenance is the optimization of existing methods and development of new techniques for microbial strain preservation, that is why the improvement of previously developed methods for authentic strain preservation is an urgent task. The article provides information on the maintenance of Burkholderia mallei 5584 (Master seed) using previously developed technique, which was supplemented with new stages in accordance with modern requirements for strain collections of highly dangerous disease agents. The previous strain maintenance technique involved its storage in its native state, which facilitated accumulation of genetic mutations and, ultimately modification of bacterial cell properties. To extend the storage time of this strain and to ensure the stability of its biological properties, the freeze-drying method was used. Skimmed milk was used as a cryoprotectant. Freeze-drying was performed under selected conditions. This technique allows for the strain sub-culturing on sensitive models once every 5 years, which is more expedient and safe from an economic and biological point of view. For safe handling of Burkholderia mallei 5584 production strain, an inactivation technique using gamma rays at 30 kGy was developed, which allowed to achieve microbial suspension sterility and preserve the bacterial cell structure. When comparing the previously developed and supplemented techniques, it was found that the improved technique of Burkholderia mallei 5584 (Master seed) maintenance makes it possible to avoid the loss of its biological properties needed for the production of high-quality laboratory diagnostic agents used for timely disease detection in susceptible animals by diagnostic tests.https://veterinary.arriah.ru/jour/article/view/796glandersburkholderia malleipassagefreeze-dryingbiological propertiesgamma irradiation |
| spellingShingle | E. A. Artemeva L. A. Melnikova L. I. Zaynullin N. K. Bukova Improved production strain maintenance technique for Burkholderia mallei 5584 (Master seed) used for glander diagnostic agent production Ветеринария сегодня glanders burkholderia mallei passage freeze-drying biological properties gamma irradiation |
| title | Improved production strain maintenance technique for Burkholderia mallei 5584 (Master seed) used for glander diagnostic agent production |
| title_full | Improved production strain maintenance technique for Burkholderia mallei 5584 (Master seed) used for glander diagnostic agent production |
| title_fullStr | Improved production strain maintenance technique for Burkholderia mallei 5584 (Master seed) used for glander diagnostic agent production |
| title_full_unstemmed | Improved production strain maintenance technique for Burkholderia mallei 5584 (Master seed) used for glander diagnostic agent production |
| title_short | Improved production strain maintenance technique for Burkholderia mallei 5584 (Master seed) used for glander diagnostic agent production |
| title_sort | improved production strain maintenance technique for burkholderia mallei 5584 master seed used for glander diagnostic agent production |
| topic | glanders burkholderia mallei passage freeze-drying biological properties gamma irradiation |
| url | https://veterinary.arriah.ru/jour/article/view/796 |
| work_keys_str_mv | AT eaartemeva improvedproductionstrainmaintenancetechniqueforburkholderiamallei5584masterseedusedforglanderdiagnosticagentproduction AT lamelnikova improvedproductionstrainmaintenancetechniqueforburkholderiamallei5584masterseedusedforglanderdiagnosticagentproduction AT lizaynullin improvedproductionstrainmaintenancetechniqueforburkholderiamallei5584masterseedusedforglanderdiagnosticagentproduction AT nkbukova improvedproductionstrainmaintenancetechniqueforburkholderiamallei5584masterseedusedforglanderdiagnosticagentproduction |