Characterization of Different Sources of Human MSCs Expanded in Serum-Free Conditions with Quantification of Chondrogenic Induction in 3D
Mesenchymal stem cells (MSCs) represent alternative candidates to chondrocytes for cartilage engineering. However, it remains difficult to identify the ideal source of MSCs for cartilage repair since conditions supporting chondrogenic induction are diverse among published works. In this study, we ch...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/2186728 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832563847344422912 |
|---|---|
| author | Hugo Fabre Maxime Ducret Olivier Degoul Jonathan Rodriguez Emeline Perrier-Groult Elisabeth Aubert-Foucher Marielle Pasdeloup Céline Auxenfans Colin McGuckin Nico Forraz Frédéric Mallein-Gerin |
| author_facet | Hugo Fabre Maxime Ducret Olivier Degoul Jonathan Rodriguez Emeline Perrier-Groult Elisabeth Aubert-Foucher Marielle Pasdeloup Céline Auxenfans Colin McGuckin Nico Forraz Frédéric Mallein-Gerin |
| author_sort | Hugo Fabre |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs) represent alternative candidates to chondrocytes for cartilage engineering. However, it remains difficult to identify the ideal source of MSCs for cartilage repair since conditions supporting chondrogenic induction are diverse among published works. In this study, we characterized and evaluated the chondrogenic potential of MSCs from bone marrow (BM), Wharton’s jelly (WJ), dental pulp (DP), and adipose tissue (AT) isolated and cultivated under serum-free conditions. BM-, WJ-, DP-, and AT-MSCs did not differ in terms of viability, clonogenicity, and proliferation. By an extensive polychromatic flow cytometry analysis, we found notable differences in markers of the osteochondrogenic lineage between the 4 MSC sources. We then evaluated their chondrogenic potential in a micromass culture model, and only BM-MSCs showed chondrogenic conversion. This chondrogenic differentiation was specifically ascertained by the production of procollagen IIB, the only type II collagen isoform synthesized by well-differentiated chondrocytes. As a pilot study toward cartilage engineering, we encapsulated BM-MSCs in hydrogel and developed an original method to evaluate their chondrogenic conversion by flow cytometry analysis, after release of the cells from the hydrogel. This allowed the simultaneous quantification of procollagen IIB and α10, a subunit of a type II collagen receptor crucial for proper cartilage development. This work represents the first comparison of detailed immunophenotypic analysis and chondrogenic differentiation potential of human BM-, WJ-, DP-, and AT-MSCs performed under the same serum-free conditions, from their isolation to their induction. Our study, achieved in conditions compliant with clinical applications, highlights that BM-MSCs are good candidates for cartilage engineering. |
| format | Article |
| id | doaj-art-6f938b70fde247f3abbf677fb681a32d |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-6f938b70fde247f3abbf677fb681a32d2025-02-03T01:12:25ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/21867282186728Characterization of Different Sources of Human MSCs Expanded in Serum-Free Conditions with Quantification of Chondrogenic Induction in 3DHugo Fabre0Maxime Ducret1Olivier Degoul2Jonathan Rodriguez3Emeline Perrier-Groult4Elisabeth Aubert-Foucher5Marielle Pasdeloup6Céline Auxenfans7Colin McGuckin8Nico Forraz9Frédéric Mallein-Gerin10Laboratory of Tissue Biology and Therapeutic Engineering, CNRS UMR 5305, University Claude Bernard-Lyon 1 and University of Lyon, 7 Passage du Vercors, 69367 Lyon, FranceLaboratory of Tissue Biology and Therapeutic Engineering, CNRS UMR 5305, University Claude Bernard-Lyon 1 and University of Lyon, 7 Passage du Vercors, 69367 Lyon, FranceCTI-BIOTECH, Cell Therapy Research Institute, 5 Avenue Lionel Terray, 69330 Meyzieu, FranceBanque de Tissus et Cellules, Laboratoire des Substituts Cutanés, Hôpital Edouard Herriot, Hospices Civils de Lyon, Pavillon I, 5 Place d’Arsonval, 69347 Lyon, FranceLaboratory of Tissue Biology and Therapeutic Engineering, CNRS UMR 5305, University Claude Bernard-Lyon 1 and University of Lyon, 7 Passage du Vercors, 69367 Lyon, FranceLaboratory of Tissue Biology and Therapeutic Engineering, CNRS UMR 5305, University Claude Bernard-Lyon 1 and University of Lyon, 7 Passage du Vercors, 69367 Lyon, FranceLaboratory of Tissue Biology and Therapeutic Engineering, CNRS UMR 5305, University Claude Bernard-Lyon 1 and University of Lyon, 7 Passage du Vercors, 69367 Lyon, FranceLaboratory of Tissue Biology and Therapeutic Engineering, CNRS UMR 5305, University Claude Bernard-Lyon 1 and University of Lyon, 7 Passage du Vercors, 69367 Lyon, FranceCTI-BIOTECH, Cell Therapy Research Institute, 5 Avenue Lionel Terray, 69330 Meyzieu, FranceCTI-BIOTECH, Cell Therapy Research Institute, 5 Avenue Lionel Terray, 69330 Meyzieu, FranceLaboratory of Tissue Biology and Therapeutic Engineering, CNRS UMR 5305, University Claude Bernard-Lyon 1 and University of Lyon, 7 Passage du Vercors, 69367 Lyon, FranceMesenchymal stem cells (MSCs) represent alternative candidates to chondrocytes for cartilage engineering. However, it remains difficult to identify the ideal source of MSCs for cartilage repair since conditions supporting chondrogenic induction are diverse among published works. In this study, we characterized and evaluated the chondrogenic potential of MSCs from bone marrow (BM), Wharton’s jelly (WJ), dental pulp (DP), and adipose tissue (AT) isolated and cultivated under serum-free conditions. BM-, WJ-, DP-, and AT-MSCs did not differ in terms of viability, clonogenicity, and proliferation. By an extensive polychromatic flow cytometry analysis, we found notable differences in markers of the osteochondrogenic lineage between the 4 MSC sources. We then evaluated their chondrogenic potential in a micromass culture model, and only BM-MSCs showed chondrogenic conversion. This chondrogenic differentiation was specifically ascertained by the production of procollagen IIB, the only type II collagen isoform synthesized by well-differentiated chondrocytes. As a pilot study toward cartilage engineering, we encapsulated BM-MSCs in hydrogel and developed an original method to evaluate their chondrogenic conversion by flow cytometry analysis, after release of the cells from the hydrogel. This allowed the simultaneous quantification of procollagen IIB and α10, a subunit of a type II collagen receptor crucial for proper cartilage development. This work represents the first comparison of detailed immunophenotypic analysis and chondrogenic differentiation potential of human BM-, WJ-, DP-, and AT-MSCs performed under the same serum-free conditions, from their isolation to their induction. Our study, achieved in conditions compliant with clinical applications, highlights that BM-MSCs are good candidates for cartilage engineering.http://dx.doi.org/10.1155/2019/2186728 |
| spellingShingle | Hugo Fabre Maxime Ducret Olivier Degoul Jonathan Rodriguez Emeline Perrier-Groult Elisabeth Aubert-Foucher Marielle Pasdeloup Céline Auxenfans Colin McGuckin Nico Forraz Frédéric Mallein-Gerin Characterization of Different Sources of Human MSCs Expanded in Serum-Free Conditions with Quantification of Chondrogenic Induction in 3D Stem Cells International |
| title | Characterization of Different Sources of Human MSCs Expanded in Serum-Free Conditions with Quantification of Chondrogenic Induction in 3D |
| title_full | Characterization of Different Sources of Human MSCs Expanded in Serum-Free Conditions with Quantification of Chondrogenic Induction in 3D |
| title_fullStr | Characterization of Different Sources of Human MSCs Expanded in Serum-Free Conditions with Quantification of Chondrogenic Induction in 3D |
| title_full_unstemmed | Characterization of Different Sources of Human MSCs Expanded in Serum-Free Conditions with Quantification of Chondrogenic Induction in 3D |
| title_short | Characterization of Different Sources of Human MSCs Expanded in Serum-Free Conditions with Quantification of Chondrogenic Induction in 3D |
| title_sort | characterization of different sources of human mscs expanded in serum free conditions with quantification of chondrogenic induction in 3d |
| url | http://dx.doi.org/10.1155/2019/2186728 |
| work_keys_str_mv | AT hugofabre characterizationofdifferentsourcesofhumanmscsexpandedinserumfreeconditionswithquantificationofchondrogenicinductionin3d AT maximeducret characterizationofdifferentsourcesofhumanmscsexpandedinserumfreeconditionswithquantificationofchondrogenicinductionin3d AT olivierdegoul characterizationofdifferentsourcesofhumanmscsexpandedinserumfreeconditionswithquantificationofchondrogenicinductionin3d AT jonathanrodriguez characterizationofdifferentsourcesofhumanmscsexpandedinserumfreeconditionswithquantificationofchondrogenicinductionin3d AT emelineperriergroult characterizationofdifferentsourcesofhumanmscsexpandedinserumfreeconditionswithquantificationofchondrogenicinductionin3d AT elisabethaubertfoucher characterizationofdifferentsourcesofhumanmscsexpandedinserumfreeconditionswithquantificationofchondrogenicinductionin3d AT mariellepasdeloup characterizationofdifferentsourcesofhumanmscsexpandedinserumfreeconditionswithquantificationofchondrogenicinductionin3d AT celineauxenfans characterizationofdifferentsourcesofhumanmscsexpandedinserumfreeconditionswithquantificationofchondrogenicinductionin3d AT colinmcguckin characterizationofdifferentsourcesofhumanmscsexpandedinserumfreeconditionswithquantificationofchondrogenicinductionin3d AT nicoforraz characterizationofdifferentsourcesofhumanmscsexpandedinserumfreeconditionswithquantificationofchondrogenicinductionin3d AT fredericmalleingerin characterizationofdifferentsourcesofhumanmscsexpandedinserumfreeconditionswithquantificationofchondrogenicinductionin3d |