A Biomimetic Approach for the Creation of Two-Dimensional Microscale Surface Patterns: Creation of Isolated Immunological Synapses
Current efforts in surface functionalization have not produced a robust technique capable of creating specific two-dimensional microscale geometrical arrays composed of multiple proteins. Such a capability is desirable for engineering substrates in sensing and cell patterning applications where at l...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2009/821308 |
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| _version_ | 1832548677738037248 |
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| author | Eric Stern David J. Mooney Tarek M. Fahmy |
| author_facet | Eric Stern David J. Mooney Tarek M. Fahmy |
| author_sort | Eric Stern |
| collection | DOAJ |
| description | Current efforts in surface functionalization have not produced a robust technique capable of creating specific two-dimensional microscale geometrical arrays composed of multiple proteins. Such a capability is desirable for engineering substrates in sensing and cell patterning applications where at least two different protein functionalities in a specific configuration are required. Here we introduce a new approach for the creation of arrays of microscale geometries. We demonstrate our approach with a biomimetic structure inspired by the immunological synapse, a cell-cell interfacial structure characterized by two concentric rings of proteins: an outer adhesion protein structure and an inner recognition ligand core. The power of the technique lies in its ability to pattern any protein in any defined geometry as well as to create arrays in parallel. |
| format | Article |
| id | doaj-art-6f92fbf3d01441eab9ae766dc74f78b7 |
| institution | Kabale University |
| issn | 1687-8787 1687-8795 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-6f92fbf3d01441eab9ae766dc74f78b72025-02-03T06:13:24ZengWileyInternational Journal of Biomaterials1687-87871687-87952009-01-01200910.1155/2009/821308821308A Biomimetic Approach for the Creation of Two-Dimensional Microscale Surface Patterns: Creation of Isolated Immunological SynapsesEric Stern0David J. Mooney1Tarek M. Fahmy2Department of Biomedical Engineering, School of Engineering and Applied Sciences, Yale University, New Haven, CT 06511, USADepartment of Bioengineering, School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USADepartment of Biomedical Engineering, School of Engineering and Applied Sciences, Yale University, New Haven, CT 06511, USACurrent efforts in surface functionalization have not produced a robust technique capable of creating specific two-dimensional microscale geometrical arrays composed of multiple proteins. Such a capability is desirable for engineering substrates in sensing and cell patterning applications where at least two different protein functionalities in a specific configuration are required. Here we introduce a new approach for the creation of arrays of microscale geometries. We demonstrate our approach with a biomimetic structure inspired by the immunological synapse, a cell-cell interfacial structure characterized by two concentric rings of proteins: an outer adhesion protein structure and an inner recognition ligand core. The power of the technique lies in its ability to pattern any protein in any defined geometry as well as to create arrays in parallel.http://dx.doi.org/10.1155/2009/821308 |
| spellingShingle | Eric Stern David J. Mooney Tarek M. Fahmy A Biomimetic Approach for the Creation of Two-Dimensional Microscale Surface Patterns: Creation of Isolated Immunological Synapses International Journal of Biomaterials |
| title | A Biomimetic Approach for the Creation of Two-Dimensional Microscale Surface Patterns: Creation of Isolated Immunological Synapses |
| title_full | A Biomimetic Approach for the Creation of Two-Dimensional Microscale Surface Patterns: Creation of Isolated Immunological Synapses |
| title_fullStr | A Biomimetic Approach for the Creation of Two-Dimensional Microscale Surface Patterns: Creation of Isolated Immunological Synapses |
| title_full_unstemmed | A Biomimetic Approach for the Creation of Two-Dimensional Microscale Surface Patterns: Creation of Isolated Immunological Synapses |
| title_short | A Biomimetic Approach for the Creation of Two-Dimensional Microscale Surface Patterns: Creation of Isolated Immunological Synapses |
| title_sort | biomimetic approach for the creation of two dimensional microscale surface patterns creation of isolated immunological synapses |
| url | http://dx.doi.org/10.1155/2009/821308 |
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