Prognostic Significance of DNA Cytometry in Carcinoma of the Uterine Cervix FIGO Stage IB and II
Objective: To assess the prognostic value of DNA‐image cytometry in cervical carcinoma of the uterus and its relation to other established prognostic factors. Study design: The study included 116 cases of cervical carcinoma FIGO stages IB and II which were treated with radical abdominal hysterectomy...
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| Format: | Article |
| Language: | English |
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Wiley
2001-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2001/602976 |
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| author | Hans Jürgen Grote Nicolaus Friedrichs Natalia Pomjanski Helen Friderike Guhde Olaf Reich Alfred Böcking |
| author_facet | Hans Jürgen Grote Nicolaus Friedrichs Natalia Pomjanski Helen Friderike Guhde Olaf Reich Alfred Böcking |
| author_sort | Hans Jürgen Grote |
| collection | DOAJ |
| description | Objective: To assess the prognostic value of DNA‐image cytometry in cervical carcinoma of the uterus and its relation to other established prognostic factors. Study design: The study included 116 cases of cervical carcinoma FIGO stages IB and II which were treated with radical abdominal hysterectomy. The median follow‐up was 55 months (range 1–162 months). DNA image cytometry was performed on cytologic specimens prepared by enzymatic cell separation from formalin‐fixed, paraffin‐embedded tissues. DNA stemline ploidy, DNA stemline aneuploidy, 5c exceeding rate, 9c exceeding rate, 2c deviation index, and DNA malignancy grade were computed. DNA‐variables as well as various clinical and histological variables were related to survival rates. Results: In multivariate statistical analysis DNA stemline ploidy using 2.2c as a cut‐off value and FIGO stage showed to be statistically significant available presurgery predictors of survival, whereas the postsurgical parameters lymphonodal status, tumor size and parametrial involvement were significantly correlated with survival. The synopsis of all parameters in a multivariate Cox model indicated that – with declining relevance – the number of positive pelvic lymph nodes, DNA stemline ploidy using a cut‐off level at a modal value of 2.2c, largest pelvic lymph node, 5c exceeding rate, and ratio of carcinoma area to cervix area, were of predictive value for survival. Conclusions: Our results suggest that prognostic information deducted from classical staging parameters is successfully complemented by DNA image cytometry which can be applied pretherapeutically. |
| format | Article |
| id | doaj-art-6ee634cfdfcc4fd9be509ec3a520cf37 |
| institution | Kabale University |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 2001-01-01 |
| publisher | Wiley |
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| series | Analytical Cellular Pathology |
| spelling | doaj-art-6ee634cfdfcc4fd9be509ec3a520cf372025-02-03T01:31:23ZengWileyAnalytical Cellular Pathology0921-89121878-36512001-01-01233-49710510.1155/2001/602976Prognostic Significance of DNA Cytometry in Carcinoma of the Uterine Cervix FIGO Stage IB and IIHans Jürgen Grote0Nicolaus Friedrichs1Natalia Pomjanski2Helen Friderike Guhde3Olaf Reich4Alfred Böcking5Institute of Cytopathology, Heinrich‐Heine‐University, Moorenstrasse 5, D‐40225 Düsseldorf, GermanyInstitute of Pathology, Friedrich‐Wilhelms‐University, Regina‐Pacis‐Weg 3, D‐53113 Bonn, GermanyInstitute of Cytopathology, Heinrich‐Heine‐University, Moorenstrasse 5, D‐40225 Düsseldorf, GermanyInstitute of Cytopathology, Heinrich‐Heine‐University, Moorenstrasse 5, D‐40225 Düsseldorf, GermanyDepartment of Obstetrics and Gynecology, Karl‐Franzens‐University, Universitätsplatz 3, A‐8010 Graz, AustriaInstitute of Cytopathology, Heinrich‐Heine‐University, Moorenstrasse 5, D‐40225 Düsseldorf, GermanyObjective: To assess the prognostic value of DNA‐image cytometry in cervical carcinoma of the uterus and its relation to other established prognostic factors. Study design: The study included 116 cases of cervical carcinoma FIGO stages IB and II which were treated with radical abdominal hysterectomy. The median follow‐up was 55 months (range 1–162 months). DNA image cytometry was performed on cytologic specimens prepared by enzymatic cell separation from formalin‐fixed, paraffin‐embedded tissues. DNA stemline ploidy, DNA stemline aneuploidy, 5c exceeding rate, 9c exceeding rate, 2c deviation index, and DNA malignancy grade were computed. DNA‐variables as well as various clinical and histological variables were related to survival rates. Results: In multivariate statistical analysis DNA stemline ploidy using 2.2c as a cut‐off value and FIGO stage showed to be statistically significant available presurgery predictors of survival, whereas the postsurgical parameters lymphonodal status, tumor size and parametrial involvement were significantly correlated with survival. The synopsis of all parameters in a multivariate Cox model indicated that – with declining relevance – the number of positive pelvic lymph nodes, DNA stemline ploidy using a cut‐off level at a modal value of 2.2c, largest pelvic lymph node, 5c exceeding rate, and ratio of carcinoma area to cervix area, were of predictive value for survival. Conclusions: Our results suggest that prognostic information deducted from classical staging parameters is successfully complemented by DNA image cytometry which can be applied pretherapeutically.http://dx.doi.org/10.1155/2001/602976 |
| spellingShingle | Hans Jürgen Grote Nicolaus Friedrichs Natalia Pomjanski Helen Friderike Guhde Olaf Reich Alfred Böcking Prognostic Significance of DNA Cytometry in Carcinoma of the Uterine Cervix FIGO Stage IB and II Analytical Cellular Pathology |
| title | Prognostic Significance of DNA Cytometry in Carcinoma of the Uterine Cervix FIGO Stage IB and II |
| title_full | Prognostic Significance of DNA Cytometry in Carcinoma of the Uterine Cervix FIGO Stage IB and II |
| title_fullStr | Prognostic Significance of DNA Cytometry in Carcinoma of the Uterine Cervix FIGO Stage IB and II |
| title_full_unstemmed | Prognostic Significance of DNA Cytometry in Carcinoma of the Uterine Cervix FIGO Stage IB and II |
| title_short | Prognostic Significance of DNA Cytometry in Carcinoma of the Uterine Cervix FIGO Stage IB and II |
| title_sort | prognostic significance of dna cytometry in carcinoma of the uterine cervix figo stage ib and ii |
| url | http://dx.doi.org/10.1155/2001/602976 |
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