Reactive Oxygen Species Generation in Human Cells by a Novel Magnetic Resonance Imaging Contrast Agent
The novel positive-contrast magnetic resonance imaging (MRI) marker C4 consists of an aqueous solution of cobalt chloride (CoCl2) complexed with the chelator N-acetylcysteine (NAC). We evaluated whether the presence of C4 or its components would produce reactive oxygen species (ROS, including hydrox...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2018/6362426 |
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| author | Li Wang Eric Lin Mary J. Johansen Timothy Madden Edward Felix Karen S. Martirosyan Steven J. Frank |
| author_facet | Li Wang Eric Lin Mary J. Johansen Timothy Madden Edward Felix Karen S. Martirosyan Steven J. Frank |
| author_sort | Li Wang |
| collection | DOAJ |
| description | The novel positive-contrast magnetic resonance imaging (MRI) marker C4 consists of an aqueous solution of cobalt chloride (CoCl2) complexed with the chelator N-acetylcysteine (NAC). We evaluated whether the presence of C4 or its components would produce reactive oxygen species (ROS, including hydroxyl, peroxyl, or other reactive oxygen species) in cultured cells. Human cancer or normal cells were incubated with 1% (w/v) CoCl2·6H2O or 2% NAC or a combination of both (1% CoCl2·6H2O : 2% NAC in an aqueous solution, abbreviated as Co : NAC) in the presence or absence of H2O2. Intracellular ROS levels were measured and quantified by change in relative fluorescence units. Student’s t-tests were used. In all cell lines exposed to 1000 μM H2O2, the Co : NAC led to ≥94.7% suppression of ROS at 5 minutes and completely suppressed ROS at 60 and 90 minutes; NAC suppressed ROS by ≥76.6% at 5 minutes and by ≥94.5% at 90 minutes; and CoCl2·6H2O suppressed ROS by ≥37.2% at 30 minutes and by ≥48.6% at 90 minutes. These results demonstrate that neither Co : NAC nor its components generated ROS; rather, they suppressed ROS production in cultured cells, suggesting that C4 would not enhance ROS production in clinical use. |
| format | Article |
| id | doaj-art-6ed50703d8d0444fa810d7b4c6a845a4 |
| institution | Kabale University |
| issn | 1687-8191 1687-8205 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
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| series | Journal of Toxicology |
| spelling | doaj-art-6ed50703d8d0444fa810d7b4c6a845a42025-02-03T00:59:30ZengWileyJournal of Toxicology1687-81911687-82052018-01-01201810.1155/2018/63624266362426Reactive Oxygen Species Generation in Human Cells by a Novel Magnetic Resonance Imaging Contrast AgentLi Wang0Eric Lin1Mary J. Johansen2Timothy Madden3Edward Felix4Karen S. Martirosyan5Steven J. Frank6Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USADepartment of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USAStrategia Therapeutics, Inc., Boston, MA, USAInPharma LLC, The Woodlands, TX, USADepartment of Experimental Therapeutics, The University of Texas MD Anderson Cancer Center, Houston, TX, USADepartment of Physics, University of Texas Rio Grande Valley, Rio Grande Valley, TX, USADepartment of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USAThe novel positive-contrast magnetic resonance imaging (MRI) marker C4 consists of an aqueous solution of cobalt chloride (CoCl2) complexed with the chelator N-acetylcysteine (NAC). We evaluated whether the presence of C4 or its components would produce reactive oxygen species (ROS, including hydroxyl, peroxyl, or other reactive oxygen species) in cultured cells. Human cancer or normal cells were incubated with 1% (w/v) CoCl2·6H2O or 2% NAC or a combination of both (1% CoCl2·6H2O : 2% NAC in an aqueous solution, abbreviated as Co : NAC) in the presence or absence of H2O2. Intracellular ROS levels were measured and quantified by change in relative fluorescence units. Student’s t-tests were used. In all cell lines exposed to 1000 μM H2O2, the Co : NAC led to ≥94.7% suppression of ROS at 5 minutes and completely suppressed ROS at 60 and 90 minutes; NAC suppressed ROS by ≥76.6% at 5 minutes and by ≥94.5% at 90 minutes; and CoCl2·6H2O suppressed ROS by ≥37.2% at 30 minutes and by ≥48.6% at 90 minutes. These results demonstrate that neither Co : NAC nor its components generated ROS; rather, they suppressed ROS production in cultured cells, suggesting that C4 would not enhance ROS production in clinical use.http://dx.doi.org/10.1155/2018/6362426 |
| spellingShingle | Li Wang Eric Lin Mary J. Johansen Timothy Madden Edward Felix Karen S. Martirosyan Steven J. Frank Reactive Oxygen Species Generation in Human Cells by a Novel Magnetic Resonance Imaging Contrast Agent Journal of Toxicology |
| title | Reactive Oxygen Species Generation in Human Cells by a Novel Magnetic Resonance Imaging Contrast Agent |
| title_full | Reactive Oxygen Species Generation in Human Cells by a Novel Magnetic Resonance Imaging Contrast Agent |
| title_fullStr | Reactive Oxygen Species Generation in Human Cells by a Novel Magnetic Resonance Imaging Contrast Agent |
| title_full_unstemmed | Reactive Oxygen Species Generation in Human Cells by a Novel Magnetic Resonance Imaging Contrast Agent |
| title_short | Reactive Oxygen Species Generation in Human Cells by a Novel Magnetic Resonance Imaging Contrast Agent |
| title_sort | reactive oxygen species generation in human cells by a novel magnetic resonance imaging contrast agent |
| url | http://dx.doi.org/10.1155/2018/6362426 |
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