The unresolved struggle of 16S rRNA amplicon sequencing: a benchmarking analysis of clustering and denoising methods
Abstract Background Although 16S rRNA gene amplicon sequencing has become an indispensable method for microbiome studies, this analysis is not error-free, and remains prone to several biases and errors. Numerous algorithms have been developed to eliminate these errors and consolidate the output into...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-05-01
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| Series: | Environmental Microbiome |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40793-025-00705-6 |
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