High and Low Annealing Temperatures Increase Both Specificity and Yield in Touchdown and Stepdown PCR
Touchdown (TD) PCR represents a versatile one-step procedure for optimizing PCRs even if the degree of primer-template complementarity is not fully known. The protocol relies on incremental annealing temperature decreases in progressive cycles designed to bracket the melting temperature (Tm) of the...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1996-03-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/19962003478 |
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