Single cell autofluorescence imaging reveals immediate metabolic shifts of neutrophils with activation across biological systems

Single cell autofluorescence imaging reveals immediate metabolic shifts of neutrophils with activation across biological systems

IntroductionNeutrophils are critical innate immune cells that heterogeneously respond to infection and inflammation by performing functions such as oxidative burst and NETosis, which require significant metabolic adaptation. Deeper insights into the single cell diversity of such metabolic changes wi...

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Main Authors: Rupsa Datta, Veronika Miskolci, Gina M. Gallego-López, Emily Britt, Amani Gillette, Aleksandr Kralovec, Morgan A. Giese, Tongcheng Qian, James Votava, Wenxuan Zhao, Jing Fan, Anna Huttenlocher, Melissa C. Skala
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-08-01
Series:Frontiers in Immunology
Subjects:
neutrophil
label-free
single-cell
optical metabolic imaging
metabolism
fluorescence lifetime imaging microscopy (FLIM)
Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2025.1617993/full
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https://www.frontiersin.org/articles/10.3389/fimmu.2025.1617993/full

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