Skin Cell and Tissue Responses to Cross-Linked Hyaluronic Acid in Low-Grade Inflammatory Conditions
Hyaluronic acid (HA), used in a variety of medical applications, is associated in rare instances to long-term adverse effects. Although the aetiology of these events is unknown, a number of hypotheses have been proposed, including low molecular weight of HA (LMW-HA) in the filler products. We hypoth...
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | International Journal of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2023/3001080 |
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| author | Benjamin Sanchez Sandra Ferraro Audrey Josset-Lamaugarny Aurélie Pagnon Charlie K. Hee Lauren Nakab Dominique Sigaudo-Roussel Bérengère Fromy |
| author_facet | Benjamin Sanchez Sandra Ferraro Audrey Josset-Lamaugarny Aurélie Pagnon Charlie K. Hee Lauren Nakab Dominique Sigaudo-Roussel Bérengère Fromy |
| author_sort | Benjamin Sanchez |
| collection | DOAJ |
| description | Hyaluronic acid (HA), used in a variety of medical applications, is associated in rare instances to long-term adverse effects. Although the aetiology of these events is unknown, a number of hypotheses have been proposed, including low molecular weight of HA (LMW-HA) in the filler products. We hypothesized that cross-linked HA and its degradation products, in a low-grade inflammatory microenvironment, could impact immune responses that could affect cell behaviours in the dermis. Using two different cross-linking technologies VYC-15L and HYC-24L+, and their hyaluronidase-induced degradation products, we observed for nondegraded HA, VYC-15L and HYC-24L+, a moderate and transient increase in IL-1β, TNF-α in M1 macrophages under low-grade inflammatory conditions. Endothelial cells and fibroblasts were preconditioned using inflammatory medium produced by M1 macrophages. 24 h after LMW-HA fragments and HA stimulation, no cytokine was released in these preconditioned cells. To further characterize HA responses, we used a novel in vivo murine model exhibiting a systemic low-grade inflammatory phenotype. The intradermal injection of VYC-15L and its degradation products induced an inflammation and cell infiltration into the skin that was more pronounced than those by HYC-24L+. This acute cutaneous inflammation was likely due to mechanical effects due to filler injection and tissue integration rather than its biological effects on inflammation. VYC-15L and its degradation product potentiated microvascular response to acetylcholine in the presence of a low-grade inflammation. The different responses with 2D cell models and mouse model using the two tested cross-linking HA technologies showed the importance to use integrative complex model to better understand the effects of HA products according to inflammatory state. |
| format | Article |
| id | doaj-art-6d4cea07a75d4e06afd09db634be387a |
| institution | Kabale University |
| issn | 2042-0099 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Inflammation |
| spelling | doaj-art-6d4cea07a75d4e06afd09db634be387a2025-02-03T06:43:16ZengWileyInternational Journal of Inflammation2042-00992023-01-01202310.1155/2023/3001080Skin Cell and Tissue Responses to Cross-Linked Hyaluronic Acid in Low-Grade Inflammatory ConditionsBenjamin Sanchez0Sandra Ferraro1Audrey Josset-Lamaugarny2Aurélie Pagnon3Charlie K. Hee4Lauren Nakab5Dominique Sigaudo-Roussel6Bérengère Fromy7Laboratoire Biologie Tissulaire et Ingénierie ThérapeutiqueLaboratoire Biologie Tissulaire et Ingénierie ThérapeutiqueLaboratoire Biologie Tissulaire et Ingénierie ThérapeutiqueNOVOTECAllergan AestheticsAllergan AestheticsLaboratoire Biologie Tissulaire et Ingénierie ThérapeutiqueLaboratoire Biologie Tissulaire et Ingénierie ThérapeutiqueHyaluronic acid (HA), used in a variety of medical applications, is associated in rare instances to long-term adverse effects. Although the aetiology of these events is unknown, a number of hypotheses have been proposed, including low molecular weight of HA (LMW-HA) in the filler products. We hypothesized that cross-linked HA and its degradation products, in a low-grade inflammatory microenvironment, could impact immune responses that could affect cell behaviours in the dermis. Using two different cross-linking technologies VYC-15L and HYC-24L+, and their hyaluronidase-induced degradation products, we observed for nondegraded HA, VYC-15L and HYC-24L+, a moderate and transient increase in IL-1β, TNF-α in M1 macrophages under low-grade inflammatory conditions. Endothelial cells and fibroblasts were preconditioned using inflammatory medium produced by M1 macrophages. 24 h after LMW-HA fragments and HA stimulation, no cytokine was released in these preconditioned cells. To further characterize HA responses, we used a novel in vivo murine model exhibiting a systemic low-grade inflammatory phenotype. The intradermal injection of VYC-15L and its degradation products induced an inflammation and cell infiltration into the skin that was more pronounced than those by HYC-24L+. This acute cutaneous inflammation was likely due to mechanical effects due to filler injection and tissue integration rather than its biological effects on inflammation. VYC-15L and its degradation product potentiated microvascular response to acetylcholine in the presence of a low-grade inflammation. The different responses with 2D cell models and mouse model using the two tested cross-linking HA technologies showed the importance to use integrative complex model to better understand the effects of HA products according to inflammatory state.http://dx.doi.org/10.1155/2023/3001080 |
| spellingShingle | Benjamin Sanchez Sandra Ferraro Audrey Josset-Lamaugarny Aurélie Pagnon Charlie K. Hee Lauren Nakab Dominique Sigaudo-Roussel Bérengère Fromy Skin Cell and Tissue Responses to Cross-Linked Hyaluronic Acid in Low-Grade Inflammatory Conditions International Journal of Inflammation |
| title | Skin Cell and Tissue Responses to Cross-Linked Hyaluronic Acid in Low-Grade Inflammatory Conditions |
| title_full | Skin Cell and Tissue Responses to Cross-Linked Hyaluronic Acid in Low-Grade Inflammatory Conditions |
| title_fullStr | Skin Cell and Tissue Responses to Cross-Linked Hyaluronic Acid in Low-Grade Inflammatory Conditions |
| title_full_unstemmed | Skin Cell and Tissue Responses to Cross-Linked Hyaluronic Acid in Low-Grade Inflammatory Conditions |
| title_short | Skin Cell and Tissue Responses to Cross-Linked Hyaluronic Acid in Low-Grade Inflammatory Conditions |
| title_sort | skin cell and tissue responses to cross linked hyaluronic acid in low grade inflammatory conditions |
| url | http://dx.doi.org/10.1155/2023/3001080 |
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