Regulation of Shade Avoidance Under Low‐Blue‐Light by MTA in Soybean
Abstract Under low blue light (LBL) conditions, soybean exhibits classic shade avoidance syndrome (SAS) with exaggerated stem elongation (ESE), leading to lodging and yield reduction in dense farming. Recently, mRNA modification by N6‐methyladenosine (m6A) has emerged as a crucial epigenetic mechani...
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| Format: | Article |
| Language: | English |
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Wiley
2025-02-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202410334 |
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| author | Liya Zhang Jun Liu Jiaqi Chen Yanyan Zhang Chao Qin Xiangguang Lyu Zhuang Li Ronghuan Ji Bin Liu Hongyu Li Tao Zhao |
| author_facet | Liya Zhang Jun Liu Jiaqi Chen Yanyan Zhang Chao Qin Xiangguang Lyu Zhuang Li Ronghuan Ji Bin Liu Hongyu Li Tao Zhao |
| author_sort | Liya Zhang |
| collection | DOAJ |
| description | Abstract Under low blue light (LBL) conditions, soybean exhibits classic shade avoidance syndrome (SAS) with exaggerated stem elongation (ESE), leading to lodging and yield reduction in dense farming. Recently, mRNA modification by N6‐methyladenosine (m6A) has emerged as a crucial epigenetic mechanism regulating plant biological processes; however, its impact on shade avoidance remains unexplored. In this study, the double mutants, gmmtas, that are impaired in two m6A writer genes, GmMTAa and GmMTAb that encode m6A methyltransferases or m6A writers are generated. It is found that the gmmtas mutants showed a substantial reduction of m6A levels, a dwarf phenotype, and a diminished sensitivity to LBL. Further investigation of the gmmtas mutants demonstrates that GmMTA regulates shade avoidance response by altering the expression of GmCRY1s, GmSPAs, and GmCOP1s, resulting in increased accumulation of GmSTFs that are known to suppress the shad avoidance response in response to LBL in soybean. The findings reveal a novel molecular mechanism regulating shade resistance in soybean, providing insights into the epigenetic mechanisms of plant adaptation to changing light environments and paving the way for the development of shade‐tolerant soybean varieties. |
| format | Article |
| id | doaj-art-6d1dff6bdc3b47b2bc1b08c78366cf0a |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-6d1dff6bdc3b47b2bc1b08c78366cf0a2025-02-04T13:14:54ZengWileyAdvanced Science2198-38442025-02-01125n/an/a10.1002/advs.202410334Regulation of Shade Avoidance Under Low‐Blue‐Light by MTA in SoybeanLiya Zhang0Jun Liu1Jiaqi Chen2Yanyan Zhang3Chao Qin4Xiangguang Lyu5Zhuang Li6Ronghuan Ji7Bin Liu8Hongyu Li9Tao Zhao10State Key Laboratory of Crop Gene Resources and BreedingKey Laboratory of Soybean Biology (Beijing) (MARA)Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081 ChinaState Key Laboratory of Crop Gene Resources and BreedingKey Laboratory of Soybean Biology (Beijing) (MARA)Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081 ChinaState Key Laboratory of Crop Gene Resources and BreedingKey Laboratory of Soybean Biology (Beijing) (MARA)Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081 ChinaState Key Laboratory of Crop Gene Resources and BreedingKey Laboratory of Soybean Biology (Beijing) (MARA)Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081 ChinaState Key Laboratory of Crop Gene Resources and BreedingKey Laboratory of Soybean Biology (Beijing) (MARA)Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081 ChinaState Key Laboratory of Crop Gene Resources and BreedingKey Laboratory of Soybean Biology (Beijing) (MARA)Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081 ChinaState Key Laboratory of Crop Gene Resources and BreedingKey Laboratory of Soybean Biology (Beijing) (MARA)Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081 ChinaState Key Laboratory of Crop Gene Resources and BreedingKey Laboratory of Soybean Biology (Beijing) (MARA)Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081 ChinaState Key Laboratory of Crop Gene Resources and BreedingKey Laboratory of Soybean Biology (Beijing) (MARA)Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081 ChinaState Key Laboratory of Crop Gene Resources and BreedingKey Laboratory of Soybean Biology (Beijing) (MARA)Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081 ChinaState Key Laboratory of Crop Gene Resources and BreedingKey Laboratory of Soybean Biology (Beijing) (MARA)Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081 ChinaAbstract Under low blue light (LBL) conditions, soybean exhibits classic shade avoidance syndrome (SAS) with exaggerated stem elongation (ESE), leading to lodging and yield reduction in dense farming. Recently, mRNA modification by N6‐methyladenosine (m6A) has emerged as a crucial epigenetic mechanism regulating plant biological processes; however, its impact on shade avoidance remains unexplored. In this study, the double mutants, gmmtas, that are impaired in two m6A writer genes, GmMTAa and GmMTAb that encode m6A methyltransferases or m6A writers are generated. It is found that the gmmtas mutants showed a substantial reduction of m6A levels, a dwarf phenotype, and a diminished sensitivity to LBL. Further investigation of the gmmtas mutants demonstrates that GmMTA regulates shade avoidance response by altering the expression of GmCRY1s, GmSPAs, and GmCOP1s, resulting in increased accumulation of GmSTFs that are known to suppress the shad avoidance response in response to LBL in soybean. The findings reveal a novel molecular mechanism regulating shade resistance in soybean, providing insights into the epigenetic mechanisms of plant adaptation to changing light environments and paving the way for the development of shade‐tolerant soybean varieties.https://doi.org/10.1002/advs.202410334blue lightMTAN6‐methyladenosine (m6A)shade avoidancesoybean |
| spellingShingle | Liya Zhang Jun Liu Jiaqi Chen Yanyan Zhang Chao Qin Xiangguang Lyu Zhuang Li Ronghuan Ji Bin Liu Hongyu Li Tao Zhao Regulation of Shade Avoidance Under Low‐Blue‐Light by MTA in Soybean Advanced Science blue light MTA N6‐methyladenosine (m6A) shade avoidance soybean |
| title | Regulation of Shade Avoidance Under Low‐Blue‐Light by MTA in Soybean |
| title_full | Regulation of Shade Avoidance Under Low‐Blue‐Light by MTA in Soybean |
| title_fullStr | Regulation of Shade Avoidance Under Low‐Blue‐Light by MTA in Soybean |
| title_full_unstemmed | Regulation of Shade Avoidance Under Low‐Blue‐Light by MTA in Soybean |
| title_short | Regulation of Shade Avoidance Under Low‐Blue‐Light by MTA in Soybean |
| title_sort | regulation of shade avoidance under low blue light by mta in soybean |
| topic | blue light MTA N6‐methyladenosine (m6A) shade avoidance soybean |
| url | https://doi.org/10.1002/advs.202410334 |
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