Lignans from Patrinia scabiosaefolia improve insulin resistance by activating PI-3K/AKT pathway and promoting GLUT4 expression
Patrinia scabiosaefolia, is used as wild vegetable in China for more than 2 000 years, with a variety of pharmacological activities, including anti-inflammatory, anti-tumor and hypoglycemic. Based on our ongoing research on chemical constituents and hypoglycemic activity of P. scabiosaefolia, 4 lign...
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| Format: | Article |
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Tsinghua University Press
2023-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023000678 |
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| author | Zhenhua Liu Mengke Wang Lijun Meng Yixiao Chen Qiuyi Wang Yan Zhang Xuefeng Xi Wenyi Kang |
| author_facet | Zhenhua Liu Mengke Wang Lijun Meng Yixiao Chen Qiuyi Wang Yan Zhang Xuefeng Xi Wenyi Kang |
| author_sort | Zhenhua Liu |
| collection | DOAJ |
| description | Patrinia scabiosaefolia, is used as wild vegetable in China for more than 2 000 years, with a variety of pharmacological activities, including anti-inflammatory, anti-tumor and hypoglycemic. Based on our ongoing research on chemical constituents and hypoglycemic activity of P. scabiosaefolia, 4 lignan compounds, (+)-isolariciresinol (1), 7R,7'R,8S,8'S-(+)-neo-olivil-4-O-β-D-glucopyranoside (2), 4-O-methylcedrusin (3) and patrinian A (4), were isolated and identified. The hypoglycemic activity showed that compounds 2 and 3 could extremely significantly improve insulin resistance at 100 (P < 0.001), 50 (P < 0.001) and 25 µmol/L (P < 0.01) in IR 3T3-L1 cells. While compound 4 only promoted glucose uptake by IR 3T3-L1 cells at 100 µmol/L (P < 0.01). Western blotting experiments showed that compounds 2 and 4 up-regulated the protein expressions of p-IRS, PI-3K, p-AKT and glucose transporter 4 (GLUT4), and promoted the transcription of GLUT4 mRNA. Therefore, the mechanisms of compounds 2 and 4 were presumed to improve IR by activating PI-3K/AKT signaling pathway. |
| format | Article |
| id | doaj-art-6d0cdd2437f0405b883ff333c7f3a1c8 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-6d0cdd2437f0405b883ff333c7f3a1c82025-02-03T06:52:48ZengTsinghua University PressFood Science and Human Wellness2213-45302023-11-0112620142021Lignans from Patrinia scabiosaefolia improve insulin resistance by activating PI-3K/AKT pathway and promoting GLUT4 expressionZhenhua Liu0Mengke Wang1Lijun Meng2Yixiao Chen3Qiuyi Wang4Yan Zhang5Xuefeng Xi6Wenyi Kang7National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; Functional Food Engineering Technology Research Center, Kaifeng 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Kaifeng 475004, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, ChinaHebei Food Inspection and Research Institute, Hebei Food Safety Key Laboratory, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Engineering Research Center for Special Food Safety and Health, Shijiazhuang 050017, China; Corresponding authors.National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; College of Physical Education, Henan University, Kaifeng 475004, China; Corresponding authors.National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; Functional Food Engineering Technology Research Center, Kaifeng 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Kaifeng 475004, China; Corresponding authors.Patrinia scabiosaefolia, is used as wild vegetable in China for more than 2 000 years, with a variety of pharmacological activities, including anti-inflammatory, anti-tumor and hypoglycemic. Based on our ongoing research on chemical constituents and hypoglycemic activity of P. scabiosaefolia, 4 lignan compounds, (+)-isolariciresinol (1), 7R,7'R,8S,8'S-(+)-neo-olivil-4-O-β-D-glucopyranoside (2), 4-O-methylcedrusin (3) and patrinian A (4), were isolated and identified. The hypoglycemic activity showed that compounds 2 and 3 could extremely significantly improve insulin resistance at 100 (P < 0.001), 50 (P < 0.001) and 25 µmol/L (P < 0.01) in IR 3T3-L1 cells. While compound 4 only promoted glucose uptake by IR 3T3-L1 cells at 100 µmol/L (P < 0.01). Western blotting experiments showed that compounds 2 and 4 up-regulated the protein expressions of p-IRS, PI-3K, p-AKT and glucose transporter 4 (GLUT4), and promoted the transcription of GLUT4 mRNA. Therefore, the mechanisms of compounds 2 and 4 were presumed to improve IR by activating PI-3K/AKT signaling pathway.http://www.sciencedirect.com/science/article/pii/S2213453023000678Patrinia scabiosaefoliaInsulin resistancePatrinian A7R,7'R,8S,8'S-(+)-neo-olivil-4-O-β-D-glucopyranoside |
| spellingShingle | Zhenhua Liu Mengke Wang Lijun Meng Yixiao Chen Qiuyi Wang Yan Zhang Xuefeng Xi Wenyi Kang Lignans from Patrinia scabiosaefolia improve insulin resistance by activating PI-3K/AKT pathway and promoting GLUT4 expression Food Science and Human Wellness Patrinia scabiosaefolia Insulin resistance Patrinian A 7R,7'R,8S,8'S-(+)-neo-olivil-4-O-β-D-glucopyranoside |
| title | Lignans from Patrinia scabiosaefolia improve insulin resistance by activating PI-3K/AKT pathway and promoting GLUT4 expression |
| title_full | Lignans from Patrinia scabiosaefolia improve insulin resistance by activating PI-3K/AKT pathway and promoting GLUT4 expression |
| title_fullStr | Lignans from Patrinia scabiosaefolia improve insulin resistance by activating PI-3K/AKT pathway and promoting GLUT4 expression |
| title_full_unstemmed | Lignans from Patrinia scabiosaefolia improve insulin resistance by activating PI-3K/AKT pathway and promoting GLUT4 expression |
| title_short | Lignans from Patrinia scabiosaefolia improve insulin resistance by activating PI-3K/AKT pathway and promoting GLUT4 expression |
| title_sort | lignans from patrinia scabiosaefolia improve insulin resistance by activating pi 3k akt pathway and promoting glut4 expression |
| topic | Patrinia scabiosaefolia Insulin resistance Patrinian A 7R,7'R,8S,8'S-(+)-neo-olivil-4-O-β-D-glucopyranoside |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023000678 |
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