Generation of a genetically encoded voltage indicator MARINA reporter human iPS cell line using Cas9 (VULSCi002-A-2)

Generation of a genetically encoded voltage indicator MARINA reporter human iPS cell line using Cas9 (VULSCi002-A-2)

Fluorescent protein-based Genetically Encoded Voltage Indicators (GEVI) offer a remarkable system for high-throughput screening of membrane potential phenotypes. The GEVI MARINA is a derivative from ArcLight, which conversely to ArcLight increases its fluorescence intensity alongside depolarization....

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Main Authors: Aistė Petruškevičiūtė, Ugnė Šimuliūnaitė, Catalina M. Polanco, Barbara Rojas, Simonas Kuras, Beatričė Valatkaitė-Rakštienė, Rimvydas Norvilas, Akshay Kumar Vijaya, Patricia Muñoz, Urtė Neniškytė, Artūras Jakubauskas, Aurelijus Burokas, Ivan Nalvarte, Jose Inzunza, Daniel Naumovas, Mindaugas Stoškus, Laimonas Griškevičius, Daiva Baltriukienė, Jonathan Arias
Format: Article
Language:English
Published: Elsevier 2025-02-01
Series:Stem Cell Research
Subjects:
Genetically encoded reporter
MARINA
Cas9
iPS cells
CRISPR
Online Access:http://www.sciencedirect.com/science/article/pii/S187350612400326X
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