PCR-based methodologies for detection and characterization of Listeria monocytogenes and Listeria ivanovii in foods and environmental sources
Listeria monocytogenes is an important foodborne pathogen responsible for listeriosis, a fatal disease. It is widely distributed in various foods and environmental sources. In this review, we focused on addressing PCR-based technologies, including conventional PCR, qPCR and droplet digital PCR (ddPC...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2017-06-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453017300125 |
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| author | Jin-Qiang Chen Stephanie Healey Patrick Regan Pongpan Laksanalamai Zonglin Hu |
| author_facet | Jin-Qiang Chen Stephanie Healey Patrick Regan Pongpan Laksanalamai Zonglin Hu |
| author_sort | Jin-Qiang Chen |
| collection | DOAJ |
| description | Listeria monocytogenes is an important foodborne pathogen responsible for listeriosis, a fatal disease. It is widely distributed in various foods and environmental sources. In this review, we focused on addressing PCR-based technologies, including conventional PCR, qPCR and droplet digital PCR (ddPCR). Specifically, we described (a) conventional PCR and mono-, duplex- and multiplex-qPCR methodologies; (b) development and applications of gene HlyA-, Iap-, PrfA – and SsrA-based conventional and qPCR assays as well as PCR assays targeting newly identified gene targets for specific detection of L. monocytogenes; differentiation of viable from dead L. monocytogenes by qPCR in conjugation with propidium monoazide pretreatment; PCR-based serotype identification of L. monocytogenes isolates; PCR-based detection of L. ivanovii, infecting ruminants, differentiation of L. monocytogenes from other Listeria species; and sigB-gene based PCR identification of Listeria spp; (c) applications of ddPCR in detection of L. monocytogenes; and (d) application of qPCR assays in detection and subtyping of L. monocytogenes in milk and dairy products; meats, meat products and meat-processing environment; and seafood, seafood products and processing environment. Our goal was to provide a relatively comprehensive overview of PCR-based methodologies available in detection, characterization and subtyping of various strains of L. monocytogenes in foods and environmental sources. |
| format | Article |
| id | doaj-art-6c82521e13cb4569bb09f98499eb358a |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2017-06-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-6c82521e13cb4569bb09f98499eb358a2025-02-03T06:15:53ZengTsinghua University PressFood Science and Human Wellness2213-45302017-06-0162395910.1016/j.fshw.2017.03.001PCR-based methodologies for detection and characterization of Listeria monocytogenes and Listeria ivanovii in foods and environmental sourcesJin-Qiang Chen0Stephanie Healey1Patrick Regan2Pongpan Laksanalamai3Zonglin Hu4Winchester Engineering & Analytical Center (WEAC), HFR-NE460, Office of Regulatory Affairs, US Food and Drug Administration, 109 Holton Street, Winchester, MA 01890, USAWinchester Engineering & Analytical Center (WEAC), HFR-NE460, Office of Regulatory Affairs, US Food and Drug Administration, 109 Holton Street, Winchester, MA 01890, USAWinchester Engineering & Analytical Center (WEAC), HFR-NE460, Office of Regulatory Affairs, US Food and Drug Administration, 109 Holton Street, Winchester, MA 01890, USAMD DHMH Laboratories Administration, Division of Microbiology, Room 528, 1770 Ashland Ave. Baltimore, MD 21205, USAWinchester Engineering & Analytical Center (WEAC), HFR-NE460, Office of Regulatory Affairs, US Food and Drug Administration, 109 Holton Street, Winchester, MA 01890, USAListeria monocytogenes is an important foodborne pathogen responsible for listeriosis, a fatal disease. It is widely distributed in various foods and environmental sources. In this review, we focused on addressing PCR-based technologies, including conventional PCR, qPCR and droplet digital PCR (ddPCR). Specifically, we described (a) conventional PCR and mono-, duplex- and multiplex-qPCR methodologies; (b) development and applications of gene HlyA-, Iap-, PrfA – and SsrA-based conventional and qPCR assays as well as PCR assays targeting newly identified gene targets for specific detection of L. monocytogenes; differentiation of viable from dead L. monocytogenes by qPCR in conjugation with propidium monoazide pretreatment; PCR-based serotype identification of L. monocytogenes isolates; PCR-based detection of L. ivanovii, infecting ruminants, differentiation of L. monocytogenes from other Listeria species; and sigB-gene based PCR identification of Listeria spp; (c) applications of ddPCR in detection of L. monocytogenes; and (d) application of qPCR assays in detection and subtyping of L. monocytogenes in milk and dairy products; meats, meat products and meat-processing environment; and seafood, seafood products and processing environment. Our goal was to provide a relatively comprehensive overview of PCR-based methodologies available in detection, characterization and subtyping of various strains of L. monocytogenes in foods and environmental sources.http://www.sciencedirect.com/science/article/pii/S2213453017300125Foodborne pathogensListeria monocytogenesListeria ivanoviiqPCR |
| spellingShingle | Jin-Qiang Chen Stephanie Healey Patrick Regan Pongpan Laksanalamai Zonglin Hu PCR-based methodologies for detection and characterization of Listeria monocytogenes and Listeria ivanovii in foods and environmental sources Food Science and Human Wellness Foodborne pathogens Listeria monocytogenes Listeria ivanovii qPCR |
| title | PCR-based methodologies for detection and characterization of Listeria monocytogenes and Listeria ivanovii in foods and environmental sources |
| title_full | PCR-based methodologies for detection and characterization of Listeria monocytogenes and Listeria ivanovii in foods and environmental sources |
| title_fullStr | PCR-based methodologies for detection and characterization of Listeria monocytogenes and Listeria ivanovii in foods and environmental sources |
| title_full_unstemmed | PCR-based methodologies for detection and characterization of Listeria monocytogenes and Listeria ivanovii in foods and environmental sources |
| title_short | PCR-based methodologies for detection and characterization of Listeria monocytogenes and Listeria ivanovii in foods and environmental sources |
| title_sort | pcr based methodologies for detection and characterization of listeria monocytogenes and listeria ivanovii in foods and environmental sources |
| topic | Foodborne pathogens Listeria monocytogenes Listeria ivanovii qPCR |
| url | http://www.sciencedirect.com/science/article/pii/S2213453017300125 |
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