Comparison of Gene Expression in Human Embryonic Stem Cells, hESC-Derived Mesenchymal Stem Cells and Human Mesenchymal Stem Cells
We present a strategy to identify developmental/differentiation and plasma membrane marker genes of the most primitive human Mesenchymal Stem Cells (hMSCs). Using sensitive and quantitative TaqMan Low Density A...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.4061/2011/368192 |
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| _version_ | 1832553753398476800 |
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| author | Romain Barbet Isabelle Peiffer Antoinette Hatzfeld Pierre Charbord Jacques A. Hatzfeld |
| author_facet | Romain Barbet Isabelle Peiffer Antoinette Hatzfeld Pierre Charbord Jacques A. Hatzfeld |
| author_sort | Romain Barbet |
| collection | DOAJ |
| description | We present a strategy to identify developmental/differentiation and
plasma membrane marker genes of the most primitive human Mesenchymal
Stem Cells (hMSCs). Using sensitive and quantitative TaqMan Low
Density Arrays (TLDA) methodology, we compared the expression of 381
genes in human Embryonic Stem Cells (hESCs), hESC-derived MSCs
(hES-MSCs), and hMSCs. Analysis of differentiation genes indicated
that hES-MSCs express the sarcomeric muscle lineage in addition to the
classical mesenchymal lineages, suggesting they are more primitive
than hMSCs. Transcript analysis of membrane antigens suggests that IL1R1low, BMPR1Blow, FLT4low, LRRC32low, and CD34 may be good candidates for the detection and
isolation of the most primitive hMSCs. The expression in hMSCs of
cytokine genes, such as IL6, IL8, or FLT3LG, without expression of the
corresponding receptor, suggests a role for these cytokines in the
paracrine control of stem cell niches. Our database may be shared with
other laboratories in order to explore the considerable clinical
potential of hES-MSCs, which appear to represent an intermediate
developmental stage between hESCs and hMSCs. |
| format | Article |
| id | doaj-art-6c464143555a4a4f8a72d3fef4cfd7ec |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-6c464143555a4a4f8a72d3fef4cfd7ec2025-02-03T05:53:19ZengWileyStem Cells International1687-966X1687-96782011-01-01201110.4061/2011/368192368192Comparison of Gene Expression in Human Embryonic Stem Cells, hESC-Derived Mesenchymal Stem Cells and Human Mesenchymal Stem CellsRomain Barbet0Isabelle Peiffer1Antoinette Hatzfeld2Pierre Charbord3Jacques A. Hatzfeld4Centre National de la Recherche Scientifique, Institut André Lwoff, 7, Rue Guy Moquet, 94800 Villejuif, FranceCentre National de la Recherche Scientifique, Institut André Lwoff, 7, Rue Guy Moquet, 94800 Villejuif, FranceCentre National de la Recherche Scientifique, Institut André Lwoff, 7, Rue Guy Moquet, 94800 Villejuif, FranceInstitut National de la Santé et Recherche Médicale U972, and University Paris 11, Hôpital de Bicêtre, 80 Avenue du Général Leclerc, 94276 Kremlin Bicêtre Cedex, FranceCentre National de la Recherche Scientifique, Institut André Lwoff, 7, Rue Guy Moquet, 94800 Villejuif, FranceWe present a strategy to identify developmental/differentiation and plasma membrane marker genes of the most primitive human Mesenchymal Stem Cells (hMSCs). Using sensitive and quantitative TaqMan Low Density Arrays (TLDA) methodology, we compared the expression of 381 genes in human Embryonic Stem Cells (hESCs), hESC-derived MSCs (hES-MSCs), and hMSCs. Analysis of differentiation genes indicated that hES-MSCs express the sarcomeric muscle lineage in addition to the classical mesenchymal lineages, suggesting they are more primitive than hMSCs. Transcript analysis of membrane antigens suggests that IL1R1low, BMPR1Blow, FLT4low, LRRC32low, and CD34 may be good candidates for the detection and isolation of the most primitive hMSCs. The expression in hMSCs of cytokine genes, such as IL6, IL8, or FLT3LG, without expression of the corresponding receptor, suggests a role for these cytokines in the paracrine control of stem cell niches. Our database may be shared with other laboratories in order to explore the considerable clinical potential of hES-MSCs, which appear to represent an intermediate developmental stage between hESCs and hMSCs.http://dx.doi.org/10.4061/2011/368192 |
| spellingShingle | Romain Barbet Isabelle Peiffer Antoinette Hatzfeld Pierre Charbord Jacques A. Hatzfeld Comparison of Gene Expression in Human Embryonic Stem Cells, hESC-Derived Mesenchymal Stem Cells and Human Mesenchymal Stem Cells Stem Cells International |
| title | Comparison of Gene Expression in Human Embryonic Stem Cells, hESC-Derived Mesenchymal Stem Cells and Human Mesenchymal Stem Cells |
| title_full | Comparison of Gene Expression in Human Embryonic Stem Cells, hESC-Derived Mesenchymal Stem Cells and Human Mesenchymal Stem Cells |
| title_fullStr | Comparison of Gene Expression in Human Embryonic Stem Cells, hESC-Derived Mesenchymal Stem Cells and Human Mesenchymal Stem Cells |
| title_full_unstemmed | Comparison of Gene Expression in Human Embryonic Stem Cells, hESC-Derived Mesenchymal Stem Cells and Human Mesenchymal Stem Cells |
| title_short | Comparison of Gene Expression in Human Embryonic Stem Cells, hESC-Derived Mesenchymal Stem Cells and Human Mesenchymal Stem Cells |
| title_sort | comparison of gene expression in human embryonic stem cells hesc derived mesenchymal stem cells and human mesenchymal stem cells |
| url | http://dx.doi.org/10.4061/2011/368192 |
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