Unveiling the amplified oxidase-mimetic activity of MnCoO nanosheets for the ultra-sensitive determination of the long-acting beta-adrenoceptor agonist indacaterol
The ability to determine indacaterol in different matrices is critical for the medication adherence assessment. In the current approach, we introduce a pioneering colorimetric detection approach for the sensitive quantitation of indacaterol using ultrathin MnCoO nanosheets with boosted oxidase-imita...
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| Language: | English |
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Elsevier
2025-08-01
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| Series: | Talanta Open |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666831925000165 |
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| author | Amal A. El-Masry Heba Elmansi Nahed El-Enany |
| author_facet | Amal A. El-Masry Heba Elmansi Nahed El-Enany |
| author_sort | Amal A. El-Masry |
| collection | DOAJ |
| description | The ability to determine indacaterol in different matrices is critical for the medication adherence assessment. In the current approach, we introduce a pioneering colorimetric detection approach for the sensitive quantitation of indacaterol using ultrathin MnCoO nanosheets with boosted oxidase-imitating properties. The nanosheets were synthesized under mild conditions through a one-step template-less simple hydrothermal process. These nanosheets demonstrated oxidase-imitating activity, enabling the change of (3,3′,5,5′-Tetramethylbenzidine ) TMB into blue-colored oxidized TMB without the need for water. Yet, the presence of indacaterol impeded this action, leading to the transformation of oxidized TMB back to colorless TMB and a subsequent reduction in blue color intensity is observed. The colorimetric reaction showed a linear correlation with indacaterol concentrations ranging from 6.0 325.0 ng/mL. This ultra sensitivity permitted reaching a detection limit down to 1.77 ng/mL. Different characterization and kinetic experiments were conducted to elucidate the underlying mechanism. Additionally, the effectiveness of the sensing platform was evaluated by measuring indacaterol levels in its pharmaceuticals. A green assessment for this approach was introduced. This well-established methodology highlights the potential of nanomaterial-based sensing technologies in pharmaceutical analysis, and related fields. |
| format | Article |
| id | doaj-art-6c38c26b45464b8baf94b043a7817bff |
| institution | Kabale University |
| issn | 2666-8319 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Talanta Open |
| spelling | doaj-art-6c38c26b45464b8baf94b043a7817bff2025-01-27T04:22:27ZengElsevierTalanta Open2666-83192025-08-0111100413Unveiling the amplified oxidase-mimetic activity of MnCoO nanosheets for the ultra-sensitive determination of the long-acting beta-adrenoceptor agonist indacaterolAmal A. El-Masry0Heba Elmansi1Nahed El-Enany2Department of Medicinal Chemistry, Faculty of Pharmacy, Mansoura University, 35516 Mansoura, EgyptDepartment of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, 35516 Mansoura, Egypt; Corresponding author.Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, 35516 Mansoura, Egypt; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, New Mansoura University, New Mansoura 7723730, EgyptThe ability to determine indacaterol in different matrices is critical for the medication adherence assessment. In the current approach, we introduce a pioneering colorimetric detection approach for the sensitive quantitation of indacaterol using ultrathin MnCoO nanosheets with boosted oxidase-imitating properties. The nanosheets were synthesized under mild conditions through a one-step template-less simple hydrothermal process. These nanosheets demonstrated oxidase-imitating activity, enabling the change of (3,3′,5,5′-Tetramethylbenzidine ) TMB into blue-colored oxidized TMB without the need for water. Yet, the presence of indacaterol impeded this action, leading to the transformation of oxidized TMB back to colorless TMB and a subsequent reduction in blue color intensity is observed. The colorimetric reaction showed a linear correlation with indacaterol concentrations ranging from 6.0 325.0 ng/mL. This ultra sensitivity permitted reaching a detection limit down to 1.77 ng/mL. Different characterization and kinetic experiments were conducted to elucidate the underlying mechanism. Additionally, the effectiveness of the sensing platform was evaluated by measuring indacaterol levels in its pharmaceuticals. A green assessment for this approach was introduced. This well-established methodology highlights the potential of nanomaterial-based sensing technologies in pharmaceutical analysis, and related fields.http://www.sciencedirect.com/science/article/pii/S2666831925000165IndacaterolColorimetryMnCoO nanosheetsTMB |
| spellingShingle | Amal A. El-Masry Heba Elmansi Nahed El-Enany Unveiling the amplified oxidase-mimetic activity of MnCoO nanosheets for the ultra-sensitive determination of the long-acting beta-adrenoceptor agonist indacaterol Talanta Open Indacaterol Colorimetry MnCoO nanosheets TMB |
| title | Unveiling the amplified oxidase-mimetic activity of MnCoO nanosheets for the ultra-sensitive determination of the long-acting beta-adrenoceptor agonist indacaterol |
| title_full | Unveiling the amplified oxidase-mimetic activity of MnCoO nanosheets for the ultra-sensitive determination of the long-acting beta-adrenoceptor agonist indacaterol |
| title_fullStr | Unveiling the amplified oxidase-mimetic activity of MnCoO nanosheets for the ultra-sensitive determination of the long-acting beta-adrenoceptor agonist indacaterol |
| title_full_unstemmed | Unveiling the amplified oxidase-mimetic activity of MnCoO nanosheets for the ultra-sensitive determination of the long-acting beta-adrenoceptor agonist indacaterol |
| title_short | Unveiling the amplified oxidase-mimetic activity of MnCoO nanosheets for the ultra-sensitive determination of the long-acting beta-adrenoceptor agonist indacaterol |
| title_sort | unveiling the amplified oxidase mimetic activity of mncoo nanosheets for the ultra sensitive determination of the long acting beta adrenoceptor agonist indacaterol |
| topic | Indacaterol Colorimetry MnCoO nanosheets TMB |
| url | http://www.sciencedirect.com/science/article/pii/S2666831925000165 |
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