High-Density Lipoprotein Reduction Differentially Modulates to Classical and Nonclassical Monocyte Subpopulations in Metabolic Syndrome Patients and in LPS-Stimulated Primary Human Monocytes In Vitro
The effect of metabolic syndrome on human monocyte subpopulations has not yet been studied. Our main goal was to examine monocyte subpopulations in metabolic syndrome patients, while also identifying the risk factors that could directly influence these cells. Eighty-six subjects were divided into me...
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2018-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2018/2737040 |
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| author | Johanna L. Grün Aaron N. Manjarrez-Reyna Angélica Y. Gómez-Arauz Sonia Leon-Cabrera Felix Rückert José M. Fragoso Nallely Bueno-Hernández Sergio Islas-Andrade Guillermo Meléndez-Mier Galileo Escobedo |
| author_facet | Johanna L. Grün Aaron N. Manjarrez-Reyna Angélica Y. Gómez-Arauz Sonia Leon-Cabrera Felix Rückert José M. Fragoso Nallely Bueno-Hernández Sergio Islas-Andrade Guillermo Meléndez-Mier Galileo Escobedo |
| author_sort | Johanna L. Grün |
| collection | DOAJ |
| description | The effect of metabolic syndrome on human monocyte subpopulations has not yet been studied. Our main goal was to examine monocyte subpopulations in metabolic syndrome patients, while also identifying the risk factors that could directly influence these cells. Eighty-six subjects were divided into metabolic syndrome patients and controls. Monocyte subpopulations were quantified by flow cytometry, and interleukin- (IL-) 1β secretion levels were measured by ELISA. Primary human monocytes were cultured in low or elevated concentrations of high-density lipoprotein (HDL) and stimulated with lipopolysaccharide (LPS). The nonclassical monocyte (NCM) percentage was significantly increased in metabolic syndrome patients as compared to controls, whereas classical monocytes (CM) were reduced. Among all metabolic syndrome risk factors, HDL reduction exhibited the most important correlation with monocyte subpopulations and then was studied in vitro. Low HDL concentration reduced the CM percentage, whereas it increased the NCM percentage and IL-1β secretion in LPS-treated monocytes. The LPS effect was abolished when monocytes were cultured in elevated HDL concentrations. Concurring with in vitro results, IL-1β serum values significantly increased in metabolic syndrome patients with low HDL levels as compared to metabolic syndrome patients without HDL reduction. Our data demonstrate that HDL directly modulates monocyte subpopulations in metabolic syndrome. |
| format | Article |
| id | doaj-art-6b84c6f33b4d4f5dbd2daff693857f08 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-6b84c6f33b4d4f5dbd2daff693857f082025-02-03T07:24:38ZengWileyJournal of Immunology Research2314-88612314-71562018-01-01201810.1155/2018/27370402737040High-Density Lipoprotein Reduction Differentially Modulates to Classical and Nonclassical Monocyte Subpopulations in Metabolic Syndrome Patients and in LPS-Stimulated Primary Human Monocytes In VitroJohanna L. Grün0Aaron N. Manjarrez-Reyna1Angélica Y. Gómez-Arauz2Sonia Leon-Cabrera3Felix Rückert4José M. Fragoso5Nallely Bueno-Hernández6Sergio Islas-Andrade7Guillermo Meléndez-Mier8Galileo Escobedo9Department of Innate Immunity and Tolerance, Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, GermanyLaboratory for Liver, Pancreas and Motility, Unit of Experimental Medicine, School of Medicine, National University of Mexico, General Hospital of Mexico “Dr. Eduardo Liceaga”, 06726 Mexico City, MexicoLaboratory for Liver, Pancreas and Motility, Unit of Experimental Medicine, School of Medicine, National University of Mexico, General Hospital of Mexico “Dr. Eduardo Liceaga”, 06726 Mexico City, MexicoCarrera de Médico Cirujano, Unidad de Biomedicina, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México, Avenida de los Barrios 1, 54090 Los Reyes Iztacala, MEX, MexicoDepartment of Surgery, University Medical Centre Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, GermanyDepartamento de Biología Molecular, Instituto Nacional de Cardiología “Ignacio Chávez”, Ciudad de México, MexicoLaboratory for Proteomics and Metabolomics, Research Division, General Hospital of Mexico “Dr. Eduardo Liceaga”, 06726 Mexico City, MexicoLaboratory for Proteomics and Metabolomics, Research Division, General Hospital of Mexico “Dr. Eduardo Liceaga”, 06726 Mexico City, MexicoLaboratory for Proteomics and Metabolomics, Research Division, General Hospital of Mexico “Dr. Eduardo Liceaga”, 06726 Mexico City, MexicoLaboratory for Liver, Pancreas and Motility, Unit of Experimental Medicine, School of Medicine, National University of Mexico, General Hospital of Mexico “Dr. Eduardo Liceaga”, 06726 Mexico City, MexicoThe effect of metabolic syndrome on human monocyte subpopulations has not yet been studied. Our main goal was to examine monocyte subpopulations in metabolic syndrome patients, while also identifying the risk factors that could directly influence these cells. Eighty-six subjects were divided into metabolic syndrome patients and controls. Monocyte subpopulations were quantified by flow cytometry, and interleukin- (IL-) 1β secretion levels were measured by ELISA. Primary human monocytes were cultured in low or elevated concentrations of high-density lipoprotein (HDL) and stimulated with lipopolysaccharide (LPS). The nonclassical monocyte (NCM) percentage was significantly increased in metabolic syndrome patients as compared to controls, whereas classical monocytes (CM) were reduced. Among all metabolic syndrome risk factors, HDL reduction exhibited the most important correlation with monocyte subpopulations and then was studied in vitro. Low HDL concentration reduced the CM percentage, whereas it increased the NCM percentage and IL-1β secretion in LPS-treated monocytes. The LPS effect was abolished when monocytes were cultured in elevated HDL concentrations. Concurring with in vitro results, IL-1β serum values significantly increased in metabolic syndrome patients with low HDL levels as compared to metabolic syndrome patients without HDL reduction. Our data demonstrate that HDL directly modulates monocyte subpopulations in metabolic syndrome.http://dx.doi.org/10.1155/2018/2737040 |
| spellingShingle | Johanna L. Grün Aaron N. Manjarrez-Reyna Angélica Y. Gómez-Arauz Sonia Leon-Cabrera Felix Rückert José M. Fragoso Nallely Bueno-Hernández Sergio Islas-Andrade Guillermo Meléndez-Mier Galileo Escobedo High-Density Lipoprotein Reduction Differentially Modulates to Classical and Nonclassical Monocyte Subpopulations in Metabolic Syndrome Patients and in LPS-Stimulated Primary Human Monocytes In Vitro Journal of Immunology Research |
| title | High-Density Lipoprotein Reduction Differentially Modulates to Classical and Nonclassical Monocyte Subpopulations in Metabolic Syndrome Patients and in LPS-Stimulated Primary Human Monocytes In Vitro |
| title_full | High-Density Lipoprotein Reduction Differentially Modulates to Classical and Nonclassical Monocyte Subpopulations in Metabolic Syndrome Patients and in LPS-Stimulated Primary Human Monocytes In Vitro |
| title_fullStr | High-Density Lipoprotein Reduction Differentially Modulates to Classical and Nonclassical Monocyte Subpopulations in Metabolic Syndrome Patients and in LPS-Stimulated Primary Human Monocytes In Vitro |
| title_full_unstemmed | High-Density Lipoprotein Reduction Differentially Modulates to Classical and Nonclassical Monocyte Subpopulations in Metabolic Syndrome Patients and in LPS-Stimulated Primary Human Monocytes In Vitro |
| title_short | High-Density Lipoprotein Reduction Differentially Modulates to Classical and Nonclassical Monocyte Subpopulations in Metabolic Syndrome Patients and in LPS-Stimulated Primary Human Monocytes In Vitro |
| title_sort | high density lipoprotein reduction differentially modulates to classical and nonclassical monocyte subpopulations in metabolic syndrome patients and in lps stimulated primary human monocytes in vitro |
| url | http://dx.doi.org/10.1155/2018/2737040 |
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