Analysis of Anthocyanins Extracted from the Acai Fruit (Euterpe oleracea): A Potential Novel Vital Dye for Chromovitrectomy
Purpose. To classify and quantify anthocyanins in a vital dye extracted from the acai fruit (Euterpe oleracea), adjust pH and osmolarity, and perform lyophilization to develop a new chromovitrectomy dye. Methods. Three dye concentrations 10%, 25%, and 35% (equivalent to 100, 250, and 350 mg of lyoph...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2018/6830835 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832556550902775808 |
|---|---|
| author | Cristiane S. Peris Rafael R. Caiado Acácio Alves Souza Lima-Filho Eduardo B. Rodrigues Michel Eid Farah Mariana Batista Gonçalves Bruno de Queiroz Alves Joao Guilherme Palma Urushima Raul Ragazzi Mauricio Maia |
| author_facet | Cristiane S. Peris Rafael R. Caiado Acácio Alves Souza Lima-Filho Eduardo B. Rodrigues Michel Eid Farah Mariana Batista Gonçalves Bruno de Queiroz Alves Joao Guilherme Palma Urushima Raul Ragazzi Mauricio Maia |
| author_sort | Cristiane S. Peris |
| collection | DOAJ |
| description | Purpose. To classify and quantify anthocyanins in a vital dye extracted from the acai fruit (Euterpe oleracea), adjust pH and osmolarity, and perform lyophilization to develop a new chromovitrectomy dye. Methods. Three dye concentrations 10%, 25%, and 35% (equivalent to 100, 250, and 350 mg of lyophilized acai fruit pulp extract samples) were evaluated when diluted in 1 ml of phosphate-buffered solution (pH 7 and 300 mOsm). The dye was analyzed by mass spectrometry and high-performance liquid chromatography (HPLC) to identify and quantify anthocyanins molecules. Results. The pH and osmolarity correction and lyophilization were performed without damaging the anthocyanin molecular structure. Mass spectrometry confirmed the presence of five anthocyanins in the three concentrations of the dye. Cyanidin-3-O-glucoside was the major anthocyanin found. HPLC showed that the concentration of anthocyanin was similar, independent of the dye concentration tested. Conclusions. Lyophilization and the correction of pH and osmolarity (7.00 and 300 mOsm, resp.) were performed successfully. Five anthocyanins are present in the dye from the acai fruit. The major anthocyanin is cyanidin-3-O-glucoside. Independent of the dye concentration tested, the anthocyanin concentration was similar. Standardized chemical characteristics of this new dye may allow its use during chromovitrectomy in humans. |
| format | Article |
| id | doaj-art-6b567c5dddc44bdd85884dcd7e0c2f2a |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-6b567c5dddc44bdd85884dcd7e0c2f2a2025-02-03T05:45:01ZengWileyJournal of Ophthalmology2090-004X2090-00582018-01-01201810.1155/2018/68308356830835Analysis of Anthocyanins Extracted from the Acai Fruit (Euterpe oleracea): A Potential Novel Vital Dye for ChromovitrectomyCristiane S. Peris0Rafael R. Caiado1Acácio Alves Souza Lima-Filho2Eduardo B. Rodrigues3Michel Eid Farah4Mariana Batista Gonçalves5Bruno de Queiroz Alves6Joao Guilherme Palma Urushima7Raul Ragazzi8Mauricio Maia9Department of Ophthalmology, Federal University of São Paulo, Botucatu Street, 816–Vila Clementino, 04023-062 São Paulo, SP, BrazilDepartment of Ophthalmology, Federal University of São Paulo, Botucatu Street, 816–Vila Clementino, 04023-062 São Paulo, SP, BrazilDepartment of Ophthalmology, Federal University of São Paulo, Botucatu Street, 816–Vila Clementino, 04023-062 São Paulo, SP, BrazilDepartment of Ophthalmology, Federal University of São Paulo, Botucatu Street, 816–Vila Clementino, 04023-062 São Paulo, SP, BrazilDepartment of Ophthalmology, Federal University of São Paulo, Botucatu Street, 816–Vila Clementino, 04023-062 São Paulo, SP, BrazilDepartment of Ophthalmology, Federal University of São Paulo, Botucatu Street, 816–Vila Clementino, 04023-062 São Paulo, SP, BrazilDepartment of Ophthalmology, Federal University of São Paulo, Botucatu Street, 816–Vila Clementino, 04023-062 São Paulo, SP, BrazilDepartment of Ophthalmology, Federal University of São Paulo, Botucatu Street, 816–Vila Clementino, 04023-062 São Paulo, SP, BrazilDepartment of Ophthalmology, Federal University of São Paulo, Botucatu Street, 816–Vila Clementino, 04023-062 São Paulo, SP, BrazilDepartment of Ophthalmology, Federal University of São Paulo, Botucatu Street, 816–Vila Clementino, 04023-062 São Paulo, SP, BrazilPurpose. To classify and quantify anthocyanins in a vital dye extracted from the acai fruit (Euterpe oleracea), adjust pH and osmolarity, and perform lyophilization to develop a new chromovitrectomy dye. Methods. Three dye concentrations 10%, 25%, and 35% (equivalent to 100, 250, and 350 mg of lyophilized acai fruit pulp extract samples) were evaluated when diluted in 1 ml of phosphate-buffered solution (pH 7 and 300 mOsm). The dye was analyzed by mass spectrometry and high-performance liquid chromatography (HPLC) to identify and quantify anthocyanins molecules. Results. The pH and osmolarity correction and lyophilization were performed without damaging the anthocyanin molecular structure. Mass spectrometry confirmed the presence of five anthocyanins in the three concentrations of the dye. Cyanidin-3-O-glucoside was the major anthocyanin found. HPLC showed that the concentration of anthocyanin was similar, independent of the dye concentration tested. Conclusions. Lyophilization and the correction of pH and osmolarity (7.00 and 300 mOsm, resp.) were performed successfully. Five anthocyanins are present in the dye from the acai fruit. The major anthocyanin is cyanidin-3-O-glucoside. Independent of the dye concentration tested, the anthocyanin concentration was similar. Standardized chemical characteristics of this new dye may allow its use during chromovitrectomy in humans.http://dx.doi.org/10.1155/2018/6830835 |
| spellingShingle | Cristiane S. Peris Rafael R. Caiado Acácio Alves Souza Lima-Filho Eduardo B. Rodrigues Michel Eid Farah Mariana Batista Gonçalves Bruno de Queiroz Alves Joao Guilherme Palma Urushima Raul Ragazzi Mauricio Maia Analysis of Anthocyanins Extracted from the Acai Fruit (Euterpe oleracea): A Potential Novel Vital Dye for Chromovitrectomy Journal of Ophthalmology |
| title | Analysis of Anthocyanins Extracted from the Acai Fruit (Euterpe oleracea): A Potential Novel Vital Dye for Chromovitrectomy |
| title_full | Analysis of Anthocyanins Extracted from the Acai Fruit (Euterpe oleracea): A Potential Novel Vital Dye for Chromovitrectomy |
| title_fullStr | Analysis of Anthocyanins Extracted from the Acai Fruit (Euterpe oleracea): A Potential Novel Vital Dye for Chromovitrectomy |
| title_full_unstemmed | Analysis of Anthocyanins Extracted from the Acai Fruit (Euterpe oleracea): A Potential Novel Vital Dye for Chromovitrectomy |
| title_short | Analysis of Anthocyanins Extracted from the Acai Fruit (Euterpe oleracea): A Potential Novel Vital Dye for Chromovitrectomy |
| title_sort | analysis of anthocyanins extracted from the acai fruit euterpe oleracea a potential novel vital dye for chromovitrectomy |
| url | http://dx.doi.org/10.1155/2018/6830835 |
| work_keys_str_mv | AT cristianesperis analysisofanthocyaninsextractedfromtheacaifruiteuterpeoleraceaapotentialnovelvitaldyeforchromovitrectomy AT rafaelrcaiado analysisofanthocyaninsextractedfromtheacaifruiteuterpeoleraceaapotentialnovelvitaldyeforchromovitrectomy AT acacioalvessouzalimafilho analysisofanthocyaninsextractedfromtheacaifruiteuterpeoleraceaapotentialnovelvitaldyeforchromovitrectomy AT eduardobrodrigues analysisofanthocyaninsextractedfromtheacaifruiteuterpeoleraceaapotentialnovelvitaldyeforchromovitrectomy AT micheleidfarah analysisofanthocyaninsextractedfromtheacaifruiteuterpeoleraceaapotentialnovelvitaldyeforchromovitrectomy AT marianabatistagoncalves analysisofanthocyaninsextractedfromtheacaifruiteuterpeoleraceaapotentialnovelvitaldyeforchromovitrectomy AT brunodequeirozalves analysisofanthocyaninsextractedfromtheacaifruiteuterpeoleraceaapotentialnovelvitaldyeforchromovitrectomy AT joaoguilhermepalmaurushima analysisofanthocyaninsextractedfromtheacaifruiteuterpeoleraceaapotentialnovelvitaldyeforchromovitrectomy AT raulragazzi analysisofanthocyaninsextractedfromtheacaifruiteuterpeoleraceaapotentialnovelvitaldyeforchromovitrectomy AT mauriciomaia analysisofanthocyaninsextractedfromtheacaifruiteuterpeoleraceaapotentialnovelvitaldyeforchromovitrectomy |