Quantitative assessment of brain metabolism in mice using non-contrast MRI at 11.7T
Brain oxygen metabolism indicates the rate of energy consumption and is a potential marker of pathological changes. Positron emission tomography (PET) is the gold standard for measuring metabolic rates using radioactive tracers. However, its application in preclinical studies, particularly with rode...
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Elsevier
2025-06-01
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| Series: | MethodsX |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016125000238 |
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| author | Xiuli Yang Yuguo Li Hanzhang Lu Zhiliang Wei |
| author_facet | Xiuli Yang Yuguo Li Hanzhang Lu Zhiliang Wei |
| author_sort | Xiuli Yang |
| collection | DOAJ |
| description | Brain oxygen metabolism indicates the rate of energy consumption and is a potential marker of pathological changes. Positron emission tomography (PET) is the gold standard for measuring metabolic rates using radioactive tracers. However, its application in preclinical studies, particularly with rodent animals, is constrained by the need for arterial input function measurements and on-site cyclotron facilities for tracer preparation. As an alternative, non-invasive, non-contrast MRI techniques, such as T2-relaxation-under-spin-tagging (TRUST) and phase-contrast (PC) MRI, can be used for evaluating brain metabolism in vivo. This study outlines a step-by-step method for implementing TRUST and PC MRI in mice at 11.7T scanner. The proposed method yields non-invasive, non-contrast quantitative measurements of global brain metabolism in approximately 20 min, paving the way for broader applications in future pathophysiological studies. • Non-invasive and non-contrast assessment of brain metabolism in mice. • Quantitative measurement of metabolic rate in approximately 20 min. |
| format | Article |
| id | doaj-art-6a1e5dd0428c43d49dde5b624ba2df9e |
| institution | Kabale University |
| issn | 2215-0161 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj-art-6a1e5dd0428c43d49dde5b624ba2df9e2025-01-27T04:21:59ZengElsevierMethodsX2215-01612025-06-0114103175Quantitative assessment of brain metabolism in mice using non-contrast MRI at 11.7TXiuli Yang0Yuguo Li1Hanzhang Lu2Zhiliang Wei3Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, United StatesDepartment of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, United StatesDepartment of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, United StatesCorresponding author.; Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, United StatesBrain oxygen metabolism indicates the rate of energy consumption and is a potential marker of pathological changes. Positron emission tomography (PET) is the gold standard for measuring metabolic rates using radioactive tracers. However, its application in preclinical studies, particularly with rodent animals, is constrained by the need for arterial input function measurements and on-site cyclotron facilities for tracer preparation. As an alternative, non-invasive, non-contrast MRI techniques, such as T2-relaxation-under-spin-tagging (TRUST) and phase-contrast (PC) MRI, can be used for evaluating brain metabolism in vivo. This study outlines a step-by-step method for implementing TRUST and PC MRI in mice at 11.7T scanner. The proposed method yields non-invasive, non-contrast quantitative measurements of global brain metabolism in approximately 20 min, paving the way for broader applications in future pathophysiological studies. • Non-invasive and non-contrast assessment of brain metabolism in mice. • Quantitative measurement of metabolic rate in approximately 20 min.http://www.sciencedirect.com/science/article/pii/S2215016125000238Cerebral metabolic rate of oxygen (CMRO2)Cerebral blood flow (CBF)Fick principleT2-relaxation-under-spin-tagging (TRUST)Phase contrast (PC) |
| spellingShingle | Xiuli Yang Yuguo Li Hanzhang Lu Zhiliang Wei Quantitative assessment of brain metabolism in mice using non-contrast MRI at 11.7T MethodsX Cerebral metabolic rate of oxygen (CMRO2) Cerebral blood flow (CBF) Fick principle T2-relaxation-under-spin-tagging (TRUST) Phase contrast (PC) |
| title | Quantitative assessment of brain metabolism in mice using non-contrast MRI at 11.7T |
| title_full | Quantitative assessment of brain metabolism in mice using non-contrast MRI at 11.7T |
| title_fullStr | Quantitative assessment of brain metabolism in mice using non-contrast MRI at 11.7T |
| title_full_unstemmed | Quantitative assessment of brain metabolism in mice using non-contrast MRI at 11.7T |
| title_short | Quantitative assessment of brain metabolism in mice using non-contrast MRI at 11.7T |
| title_sort | quantitative assessment of brain metabolism in mice using non contrast mri at 11 7t |
| topic | Cerebral metabolic rate of oxygen (CMRO2) Cerebral blood flow (CBF) Fick principle T2-relaxation-under-spin-tagging (TRUST) Phase contrast (PC) |
| url | http://www.sciencedirect.com/science/article/pii/S2215016125000238 |
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