Establishment of a UPLC-MS/MS Method for Studying the Effect of Salt-Processing on Tissue Distribution of Twelve Major Bioactive Components of Qing’e Pills in Rats
Qing’e pills is clinically used for treating osteoporosis in postmenopausal women in China. Eucommiae Cortex and Psoraleae Fructus are the main herbs of Qing’e pills and are both required to be salt-processed. In order to study the influence of salt-processing on the tissue distribution of Qing’e pi...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2020/8832736 |
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| author | Jingxia Hou Shangyang Lin Jinlan Lu Yu Wu Li Wu Zhipeng Chen Weidong Li |
| author_facet | Jingxia Hou Shangyang Lin Jinlan Lu Yu Wu Li Wu Zhipeng Chen Weidong Li |
| author_sort | Jingxia Hou |
| collection | DOAJ |
| description | Qing’e pills is clinically used for treating osteoporosis in postmenopausal women in China. Eucommiae Cortex and Psoraleae Fructus are the main herbs of Qing’e pills and are both required to be salt-processed. In order to study the influence of salt-processing on the tissue distribution of Qing’e pills, a UPLC-MS/MS method was established for studying the tissue distribution of 12 main bioactive ingredients of Qing’e pills in rats. The linear relationships of the 12 compounds in each tissue were good. The method was fully validated for its selectivity, accuracy, precision, stability, matrix effect, and extraction recovery. Then, the validated method was successfully applied for simultaneous determination of the 12 chemical components in Qing’e pills in tissues for the first time. Areas under the curve (AUC) results showed that, except for pinoresinol diglucoside, psoralen, and isopsoralen, the distribution of the other components was increased in the kidney, uterus, ovary, and testes. Relative targeting efficiency (RTE) results showed that all 12 chemical components targeted the kidney and sexual organs. The results indicated that the Eucommiae Cortex and Psoraleae Fructus after salt-processing could significantly increase the distribution of components to the kidney and generative organs. |
| format | Article |
| id | doaj-art-68acf0aad5cb4c8788bb50adaa661008 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-68acf0aad5cb4c8788bb50adaa6610082025-02-03T06:04:35ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732020-01-01202010.1155/2020/88327368832736Establishment of a UPLC-MS/MS Method for Studying the Effect of Salt-Processing on Tissue Distribution of Twelve Major Bioactive Components of Qing’e Pills in RatsJingxia Hou0Shangyang Lin1Jinlan Lu2Yu Wu3Li Wu4Zhipeng Chen5Weidong Li6College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaQing’e pills is clinically used for treating osteoporosis in postmenopausal women in China. Eucommiae Cortex and Psoraleae Fructus are the main herbs of Qing’e pills and are both required to be salt-processed. In order to study the influence of salt-processing on the tissue distribution of Qing’e pills, a UPLC-MS/MS method was established for studying the tissue distribution of 12 main bioactive ingredients of Qing’e pills in rats. The linear relationships of the 12 compounds in each tissue were good. The method was fully validated for its selectivity, accuracy, precision, stability, matrix effect, and extraction recovery. Then, the validated method was successfully applied for simultaneous determination of the 12 chemical components in Qing’e pills in tissues for the first time. Areas under the curve (AUC) results showed that, except for pinoresinol diglucoside, psoralen, and isopsoralen, the distribution of the other components was increased in the kidney, uterus, ovary, and testes. Relative targeting efficiency (RTE) results showed that all 12 chemical components targeted the kidney and sexual organs. The results indicated that the Eucommiae Cortex and Psoraleae Fructus after salt-processing could significantly increase the distribution of components to the kidney and generative organs.http://dx.doi.org/10.1155/2020/8832736 |
| spellingShingle | Jingxia Hou Shangyang Lin Jinlan Lu Yu Wu Li Wu Zhipeng Chen Weidong Li Establishment of a UPLC-MS/MS Method for Studying the Effect of Salt-Processing on Tissue Distribution of Twelve Major Bioactive Components of Qing’e Pills in Rats Journal of Analytical Methods in Chemistry |
| title | Establishment of a UPLC-MS/MS Method for Studying the Effect of Salt-Processing on Tissue Distribution of Twelve Major Bioactive Components of Qing’e Pills in Rats |
| title_full | Establishment of a UPLC-MS/MS Method for Studying the Effect of Salt-Processing on Tissue Distribution of Twelve Major Bioactive Components of Qing’e Pills in Rats |
| title_fullStr | Establishment of a UPLC-MS/MS Method for Studying the Effect of Salt-Processing on Tissue Distribution of Twelve Major Bioactive Components of Qing’e Pills in Rats |
| title_full_unstemmed | Establishment of a UPLC-MS/MS Method for Studying the Effect of Salt-Processing on Tissue Distribution of Twelve Major Bioactive Components of Qing’e Pills in Rats |
| title_short | Establishment of a UPLC-MS/MS Method for Studying the Effect of Salt-Processing on Tissue Distribution of Twelve Major Bioactive Components of Qing’e Pills in Rats |
| title_sort | establishment of a uplc ms ms method for studying the effect of salt processing on tissue distribution of twelve major bioactive components of qing e pills in rats |
| url | http://dx.doi.org/10.1155/2020/8832736 |
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