Development of a HPLC Method for the Quantitative Determination of Capsaicin in Collagen Sponge
Controlling the concentration of drugs in pharmaceutical products is essential to patient’s safety. In this study, a simple and sensitive HPLC method is developed to quantitatively analyze capsaicin in collagen sponge. The capsaicin from sponge was extracted for 30 min with ultrasonic wave extractio...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/912631 |
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| author | Chun-Lian Guo Hong-Ying Chen Bi-Ling Cui Yu-Huan Chen Yan-Fang Zhou Xin-Sheng Peng Qin Wang |
| author_facet | Chun-Lian Guo Hong-Ying Chen Bi-Ling Cui Yu-Huan Chen Yan-Fang Zhou Xin-Sheng Peng Qin Wang |
| author_sort | Chun-Lian Guo |
| collection | DOAJ |
| description | Controlling the concentration of drugs in pharmaceutical products is essential to patient’s safety. In this study, a simple and sensitive HPLC method is developed to quantitatively analyze capsaicin in collagen sponge. The capsaicin from sponge was extracted for 30 min with ultrasonic wave extraction technique and methanol was used as solvent. The chromatographic method was performed by using isocratic system composed of acetonitrile-water (70 : 30) with a flow rate of 1 mL/min and the detection wavelength was at 280 nm. Capsaicin can be successfully separated with good linearity (the regression equation is A = 9.7182C + 0.8547; R2 = 1.0) and perfect recovery (99.72%). The mean capsaicin concentration in collagen sponge was 49.32 mg/g (RSD = 1.30%; n = 3). In conclusion, the ultrasonic wave extraction method is simple and the extracting efficiency is high. The HPLC assay has excellent sensitivity and specificity and is a convenient method for capsaicin detection in collagen sponge. This paper firstly discusses the quantitative analysis of capsaicin in collagen sponge. |
| format | Article |
| id | doaj-art-688bc4da3c5c4e0b8b865d8e055a8f6f |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-688bc4da3c5c4e0b8b865d8e055a8f6f2025-02-03T05:58:41ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792015-01-01201510.1155/2015/912631912631Development of a HPLC Method for the Quantitative Determination of Capsaicin in Collagen SpongeChun-Lian Guo0Hong-Ying Chen1Bi-Ling Cui2Yu-Huan Chen3Yan-Fang Zhou4Xin-Sheng Peng5Qin Wang6School of Pharmacy, Guangdong Medical University, Dongguan, Guangdong 523808, ChinaSchool of Pharmacy, Guangdong Medical University, Dongguan, Guangdong 523808, ChinaSchool of Pharmacy, Guangdong Medical University, Dongguan, Guangdong 523808, ChinaSchool of Pharmacy, Guangdong Medical University, Dongguan, Guangdong 523808, ChinaSchool of Pharmacy, Guangdong Medical University, Dongguan, Guangdong 523808, ChinaSchool of Pharmacy, Guangdong Medical University, Dongguan, Guangdong 523808, ChinaSchool of Pharmacy, Guangdong Medical University, Dongguan, Guangdong 523808, ChinaControlling the concentration of drugs in pharmaceutical products is essential to patient’s safety. In this study, a simple and sensitive HPLC method is developed to quantitatively analyze capsaicin in collagen sponge. The capsaicin from sponge was extracted for 30 min with ultrasonic wave extraction technique and methanol was used as solvent. The chromatographic method was performed by using isocratic system composed of acetonitrile-water (70 : 30) with a flow rate of 1 mL/min and the detection wavelength was at 280 nm. Capsaicin can be successfully separated with good linearity (the regression equation is A = 9.7182C + 0.8547; R2 = 1.0) and perfect recovery (99.72%). The mean capsaicin concentration in collagen sponge was 49.32 mg/g (RSD = 1.30%; n = 3). In conclusion, the ultrasonic wave extraction method is simple and the extracting efficiency is high. The HPLC assay has excellent sensitivity and specificity and is a convenient method for capsaicin detection in collagen sponge. This paper firstly discusses the quantitative analysis of capsaicin in collagen sponge.http://dx.doi.org/10.1155/2015/912631 |
| spellingShingle | Chun-Lian Guo Hong-Ying Chen Bi-Ling Cui Yu-Huan Chen Yan-Fang Zhou Xin-Sheng Peng Qin Wang Development of a HPLC Method for the Quantitative Determination of Capsaicin in Collagen Sponge International Journal of Analytical Chemistry |
| title | Development of a HPLC Method for the Quantitative Determination of Capsaicin in Collagen Sponge |
| title_full | Development of a HPLC Method for the Quantitative Determination of Capsaicin in Collagen Sponge |
| title_fullStr | Development of a HPLC Method for the Quantitative Determination of Capsaicin in Collagen Sponge |
| title_full_unstemmed | Development of a HPLC Method for the Quantitative Determination of Capsaicin in Collagen Sponge |
| title_short | Development of a HPLC Method for the Quantitative Determination of Capsaicin in Collagen Sponge |
| title_sort | development of a hplc method for the quantitative determination of capsaicin in collagen sponge |
| url | http://dx.doi.org/10.1155/2015/912631 |
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