Impairments of Antigen-Presenting Cells in Pulmonary Tuberculosis
The phenotype and functional properties of antigen-presenting cells (APC), that is, circulating monocytes and generated in vitro macrophages and dendritic cells, were investigated in the patients with pulmonary tuberculosis (TB) differing in lymphocyte reactivity to M. tuberculosis antigens (PPD-rea...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/793292 |
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| author | Ludmila V. Sakhno Ekaterina Ya. Shevela Marina A. Tikhonova Sergey D. Nikonov Alexandr A. Ostanin Elena R. Chernykh |
| author_facet | Ludmila V. Sakhno Ekaterina Ya. Shevela Marina A. Tikhonova Sergey D. Nikonov Alexandr A. Ostanin Elena R. Chernykh |
| author_sort | Ludmila V. Sakhno |
| collection | DOAJ |
| description | The phenotype and functional properties of antigen-presenting cells (APC), that is, circulating monocytes and generated in vitro macrophages and dendritic cells, were investigated in the patients with pulmonary tuberculosis (TB) differing in lymphocyte reactivity to M. tuberculosis antigens (PPD-reactive versus PPD-anergic patients). We revealed the distinct impairments in patient APC functions. For example, the monocyte dysfunctions were displayed by low CD86 and HLA-DR expression, 2-fold increase in CD14+CD16+ expression, the high numbers of IL-10-producing cells, and enhanced IL-10 and IL-6 production upon LPS-stimulation. The macrophages which were in vitro generated from peripheral blood monocytes under GM-CSF were characterized by Th1/Th2-balance shifting (downproduction of IFN-γ coupled with upproduction of IL-10) and by reducing of allostimulatory activity in mixed lymphocyte culture. The dendritic cells (generated in vitro from peripheral blood monocytes upon GM-CSF + IFN-α) were characterized by impaired maturation/activation, a lower level of IFN-γ production in conjunction with an enhanced capacity to produce IL-10 and IL-6, and a profound reduction of allostimulatory activity. The APC dysfunctions were found to be most prominent in PPD-anergic patients. The possible role of APC impairments in reducing the antigen-specific T-cell response to M. tuberculosis was discussed. |
| format | Article |
| id | doaj-art-6769d298074f4817820e9e3859eebc49 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-6769d298074f4817820e9e3859eebc492025-02-03T05:51:52ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/793292793292Impairments of Antigen-Presenting Cells in Pulmonary TuberculosisLudmila V. Sakhno0Ekaterina Ya. Shevela1Marina A. Tikhonova2Sergey D. Nikonov3Alexandr A. Ostanin4Elena R. Chernykh5Research Institute of Clinical Immunology, Russian Academy of Medical Sciences (RAMS), Siberian Branch (SB), Yadrintsevskaya Street 14, Novosibirsk 630099, RussiaResearch Institute of Clinical Immunology, Russian Academy of Medical Sciences (RAMS), Siberian Branch (SB), Yadrintsevskaya Street 14, Novosibirsk 630099, RussiaResearch Institute of Clinical Immunology, Russian Academy of Medical Sciences (RAMS), Siberian Branch (SB), Yadrintsevskaya Street 14, Novosibirsk 630099, RussiaNovosibirsk Tuberculosis Clinical Hospital No. 1, Vavilova Street 14, Novosibirsk 630082, RussiaResearch Institute of Clinical Immunology, Russian Academy of Medical Sciences (RAMS), Siberian Branch (SB), Yadrintsevskaya Street 14, Novosibirsk 630099, RussiaResearch Institute of Clinical Immunology, Russian Academy of Medical Sciences (RAMS), Siberian Branch (SB), Yadrintsevskaya Street 14, Novosibirsk 630099, RussiaThe phenotype and functional properties of antigen-presenting cells (APC), that is, circulating monocytes and generated in vitro macrophages and dendritic cells, were investigated in the patients with pulmonary tuberculosis (TB) differing in lymphocyte reactivity to M. tuberculosis antigens (PPD-reactive versus PPD-anergic patients). We revealed the distinct impairments in patient APC functions. For example, the monocyte dysfunctions were displayed by low CD86 and HLA-DR expression, 2-fold increase in CD14+CD16+ expression, the high numbers of IL-10-producing cells, and enhanced IL-10 and IL-6 production upon LPS-stimulation. The macrophages which were in vitro generated from peripheral blood monocytes under GM-CSF were characterized by Th1/Th2-balance shifting (downproduction of IFN-γ coupled with upproduction of IL-10) and by reducing of allostimulatory activity in mixed lymphocyte culture. The dendritic cells (generated in vitro from peripheral blood monocytes upon GM-CSF + IFN-α) were characterized by impaired maturation/activation, a lower level of IFN-γ production in conjunction with an enhanced capacity to produce IL-10 and IL-6, and a profound reduction of allostimulatory activity. The APC dysfunctions were found to be most prominent in PPD-anergic patients. The possible role of APC impairments in reducing the antigen-specific T-cell response to M. tuberculosis was discussed.http://dx.doi.org/10.1155/2015/793292 |
| spellingShingle | Ludmila V. Sakhno Ekaterina Ya. Shevela Marina A. Tikhonova Sergey D. Nikonov Alexandr A. Ostanin Elena R. Chernykh Impairments of Antigen-Presenting Cells in Pulmonary Tuberculosis Journal of Immunology Research |
| title | Impairments of Antigen-Presenting Cells in Pulmonary Tuberculosis |
| title_full | Impairments of Antigen-Presenting Cells in Pulmonary Tuberculosis |
| title_fullStr | Impairments of Antigen-Presenting Cells in Pulmonary Tuberculosis |
| title_full_unstemmed | Impairments of Antigen-Presenting Cells in Pulmonary Tuberculosis |
| title_short | Impairments of Antigen-Presenting Cells in Pulmonary Tuberculosis |
| title_sort | impairments of antigen presenting cells in pulmonary tuberculosis |
| url | http://dx.doi.org/10.1155/2015/793292 |
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