Shedding Light on Luminescence Lifetime Measurement and Associated Data Treatment

Luminescence lifetime is a crucial parameter in photophysical studies that bears essential physical and chemical information and that is used to quantify a variety of phenomena, from the determination of quenching mechanisms to temperature sensing and bioimaging. The current perception of lifetime m...

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Main Authors: Waygen Thor, Jean‐Claude G. Bünzli, Ka‐Leung Wong, Peter A. Tanner
Format: Article
Language:English
Published: Wiley-VCH 2025-02-01
Series:Advanced Photonics Research
Subjects:
Online Access:https://doi.org/10.1002/adpr.202400081
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author Waygen Thor
Jean‐Claude G. Bünzli
Ka‐Leung Wong
Peter A. Tanner
author_facet Waygen Thor
Jean‐Claude G. Bünzli
Ka‐Leung Wong
Peter A. Tanner
author_sort Waygen Thor
collection DOAJ
description Luminescence lifetime is a crucial parameter in photophysical studies that bears essential physical and chemical information and that is used to quantify a variety of phenomena, from the determination of quenching mechanisms to temperature sensing and bioimaging. The current perception of lifetime measurement is that it is a trivial and fast experiment. However, despite this apparent simplicity, measuring luminescence decay and fitting the obtained data to a suitable model can be far more intricate. In this perspective, the influence of experimental parameters and fitting procedures on the determination of lifetimes are investigated and, through carefully chosen examples, it is shown that large variations, up to 10%, can be induced by varying parameters such as the data acquisition time, the baseline evaluation, or the mathematical fitting model. In order to present to a wider audience, detailed mathematical descriptions are kept out of the manuscript.
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institution Kabale University
issn 2699-9293
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series Advanced Photonics Research
spelling doaj-art-66e565eb5a804cd5b217e7decfaab0fe2025-02-06T08:56:39ZengWiley-VCHAdvanced Photonics Research2699-92932025-02-0162n/an/a10.1002/adpr.202400081Shedding Light on Luminescence Lifetime Measurement and Associated Data TreatmentWaygen Thor0Jean‐Claude G. Bünzli1Ka‐Leung Wong2Peter A. Tanner3Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic University Kowloon Hong Kong S.A.R.Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic University Kowloon Hong Kong S.A.R.Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic University Kowloon Hong Kong S.A.R.Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic University Kowloon Hong Kong S.A.R.Luminescence lifetime is a crucial parameter in photophysical studies that bears essential physical and chemical information and that is used to quantify a variety of phenomena, from the determination of quenching mechanisms to temperature sensing and bioimaging. The current perception of lifetime measurement is that it is a trivial and fast experiment. However, despite this apparent simplicity, measuring luminescence decay and fitting the obtained data to a suitable model can be far more intricate. In this perspective, the influence of experimental parameters and fitting procedures on the determination of lifetimes are investigated and, through carefully chosen examples, it is shown that large variations, up to 10%, can be induced by varying parameters such as the data acquisition time, the baseline evaluation, or the mathematical fitting model. In order to present to a wider audience, detailed mathematical descriptions are kept out of the manuscript.https://doi.org/10.1002/adpr.202400081experimental parametersfitting procedurekinetic modellifetimeluminescence
spellingShingle Waygen Thor
Jean‐Claude G. Bünzli
Ka‐Leung Wong
Peter A. Tanner
Shedding Light on Luminescence Lifetime Measurement and Associated Data Treatment
Advanced Photonics Research
experimental parameters
fitting procedure
kinetic model
lifetime
luminescence
title Shedding Light on Luminescence Lifetime Measurement and Associated Data Treatment
title_full Shedding Light on Luminescence Lifetime Measurement and Associated Data Treatment
title_fullStr Shedding Light on Luminescence Lifetime Measurement and Associated Data Treatment
title_full_unstemmed Shedding Light on Luminescence Lifetime Measurement and Associated Data Treatment
title_short Shedding Light on Luminescence Lifetime Measurement and Associated Data Treatment
title_sort shedding light on luminescence lifetime measurement and associated data treatment
topic experimental parameters
fitting procedure
kinetic model
lifetime
luminescence
url https://doi.org/10.1002/adpr.202400081
work_keys_str_mv AT waygenthor sheddinglightonluminescencelifetimemeasurementandassociateddatatreatment
AT jeanclaudegbunzli sheddinglightonluminescencelifetimemeasurementandassociateddatatreatment
AT kaleungwong sheddinglightonluminescencelifetimemeasurementandassociateddatatreatment
AT peteratanner sheddinglightonluminescencelifetimemeasurementandassociateddatatreatment