乙肝病毒PCR产物的鉴定及单项抗HBc阳性血清的HBV基因扩增
本文报道ＨＢＶ基因扩增产物的限制性酶切分析和寡核苷酸探针杂交鉴定、应用血清ＨＢＶＤＮＡ的ＰＣＲ扩增产物制备基因探针和单项抗ＨＢｃ阳性血清的ＨＢＶ基因扩增。ＰＣＲ用ａｄｒ、ａｄｗ、ａｙｗ３种亚型ＨＢＶ的Ｃ基因区共有序列为内外两对引物分单次或套式扩增，产物为６６ａｂｐ或４２８ｂｐ，两者序列中段共含－ＢｇＩⅡ酶切点并与一寡核苷酸探针同源。４２８ｂｐ产物被用以缺口平移标记３２Ｐ探针作斑点和转印杂交检测。结合分子杂交的ＰＣＲ结果从单项抗ＨＢｃ阳性的８／４２例慢性肝炎和２／１２例无症状受测者血清中检出ＨＢＶＤＮＡ，证实病毒低度复制和传染性。...
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| Format: | Article |
|---|---|
| Language: | zho |
| Published: |
Editorial Office of Journal of Sun Yat-sen University
1994-01-01
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| Series: | Zhongshan Daxue xuebao. Yixue kexue ban |
| Subjects: | |
| Online Access: | http://xuebaoyx.sysu.edu.cn/zh/article/43592285/ |
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