Low Ethanol Concentrations Promote Endothelial Progenitor Cell Capacity and Reparative Function
Background. Endothelial progenitor cells (EPCs) are recruited to injured endothelium and contribute to its regeneration. There is evidence that moderate ethanol consumption prevents the development and progression of atherosclerosis in a variety of in vitro and in vivo models and increases the mobil...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Cardiovascular Therapeutics |
| Online Access: | http://dx.doi.org/10.1155/2020/4018478 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832566481750065152 |
|---|---|
| author | Lars Brodowski Bianca Schröder-Heurich Berina Kipke Cara Schmidt Constantin S. von Kaisenberg Frauke von Versen-Höynck |
| author_facet | Lars Brodowski Bianca Schröder-Heurich Berina Kipke Cara Schmidt Constantin S. von Kaisenberg Frauke von Versen-Höynck |
| author_sort | Lars Brodowski |
| collection | DOAJ |
| description | Background. Endothelial progenitor cells (EPCs) are recruited to injured endothelium and contribute to its regeneration. There is evidence that moderate ethanol consumption prevents the development and progression of atherosclerosis in a variety of in vitro and in vivo models and increases the mobilization of progenitor cells. Furthermore, there are studies that identified ethanol at low concentration as a therapeutic tool to mobilize progenitor cells in peripheral blood. At the same time, the cell number of EPCs represents a close link to cardiovascular system constitution and function and contributes to cardiovascular risk. The aim of this study was to evaluate the effect of low dose ethanol on typical features of endothelial colony-forming cells (ECFCs), a proliferative subtype of EPCs. Methods and Results. We tested whether ethanol impacts the functional abilities of ECFC (e.g., migration, tube formation, and proliferation) using in vitro assays, the intercommunication of ECFC by exploring cell surface molecules by flow cytometry, and the expression of (anti-)angiogenic molecules by ELISA. Low concentrations of ethanol concentration promoted migration, proliferation, and tubule formation of ECFC. The expression of the cell surface marker VE-cadherin, a protein which plays an important role in cell-cell interaction, was enhanced by ethanol, while (anti-)angiogenic molecule expression was not impacted. Conclusion. Ethanol at moderate concentrations increases the angiogenic abilities of endothelial progenitor cells thus possibly contributing to vasoprotection. |
| format | Article |
| id | doaj-art-6557043b4b714e4b86da5f9cbc9d13c2 |
| institution | Kabale University |
| issn | 1755-5914 1755-5922 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cardiovascular Therapeutics |
| spelling | doaj-art-6557043b4b714e4b86da5f9cbc9d13c22025-02-03T01:03:58ZengWileyCardiovascular Therapeutics1755-59141755-59222020-01-01202010.1155/2020/40184784018478Low Ethanol Concentrations Promote Endothelial Progenitor Cell Capacity and Reparative FunctionLars Brodowski0Bianca Schröder-Heurich1Berina Kipke2Cara Schmidt3Constantin S. von Kaisenberg4Frauke von Versen-Höynck5Gynecology Research Unit, Hannover Medical School, Carl-Neuberg-Strasse 1, D-30625 Hannover, GermanyGynecology Research Unit, Hannover Medical School, Carl-Neuberg-Strasse 1, D-30625 Hannover, GermanyGynecology Research Unit, Hannover Medical School, Carl-Neuberg-Strasse 1, D-30625 Hannover, GermanyGynecology Research Unit, Hannover Medical School, Carl-Neuberg-Strasse 1, D-30625 Hannover, GermanyDepartment of Obstetrics and Gynecology, Hannover Medical School, Carl-Neuberg-Strasse 1, D-30625 Hannover, GermanyGynecology Research Unit, Hannover Medical School, Carl-Neuberg-Strasse 1, D-30625 Hannover, GermanyBackground. Endothelial progenitor cells (EPCs) are recruited to injured endothelium and contribute to its regeneration. There is evidence that moderate ethanol consumption prevents the development and progression of atherosclerosis in a variety of in vitro and in vivo models and increases the mobilization of progenitor cells. Furthermore, there are studies that identified ethanol at low concentration as a therapeutic tool to mobilize progenitor cells in peripheral blood. At the same time, the cell number of EPCs represents a close link to cardiovascular system constitution and function and contributes to cardiovascular risk. The aim of this study was to evaluate the effect of low dose ethanol on typical features of endothelial colony-forming cells (ECFCs), a proliferative subtype of EPCs. Methods and Results. We tested whether ethanol impacts the functional abilities of ECFC (e.g., migration, tube formation, and proliferation) using in vitro assays, the intercommunication of ECFC by exploring cell surface molecules by flow cytometry, and the expression of (anti-)angiogenic molecules by ELISA. Low concentrations of ethanol concentration promoted migration, proliferation, and tubule formation of ECFC. The expression of the cell surface marker VE-cadherin, a protein which plays an important role in cell-cell interaction, was enhanced by ethanol, while (anti-)angiogenic molecule expression was not impacted. Conclusion. Ethanol at moderate concentrations increases the angiogenic abilities of endothelial progenitor cells thus possibly contributing to vasoprotection.http://dx.doi.org/10.1155/2020/4018478 |
| spellingShingle | Lars Brodowski Bianca Schröder-Heurich Berina Kipke Cara Schmidt Constantin S. von Kaisenberg Frauke von Versen-Höynck Low Ethanol Concentrations Promote Endothelial Progenitor Cell Capacity and Reparative Function Cardiovascular Therapeutics |
| title | Low Ethanol Concentrations Promote Endothelial Progenitor Cell Capacity and Reparative Function |
| title_full | Low Ethanol Concentrations Promote Endothelial Progenitor Cell Capacity and Reparative Function |
| title_fullStr | Low Ethanol Concentrations Promote Endothelial Progenitor Cell Capacity and Reparative Function |
| title_full_unstemmed | Low Ethanol Concentrations Promote Endothelial Progenitor Cell Capacity and Reparative Function |
| title_short | Low Ethanol Concentrations Promote Endothelial Progenitor Cell Capacity and Reparative Function |
| title_sort | low ethanol concentrations promote endothelial progenitor cell capacity and reparative function |
| url | http://dx.doi.org/10.1155/2020/4018478 |
| work_keys_str_mv | AT larsbrodowski lowethanolconcentrationspromoteendothelialprogenitorcellcapacityandreparativefunction AT biancaschroderheurich lowethanolconcentrationspromoteendothelialprogenitorcellcapacityandreparativefunction AT berinakipke lowethanolconcentrationspromoteendothelialprogenitorcellcapacityandreparativefunction AT caraschmidt lowethanolconcentrationspromoteendothelialprogenitorcellcapacityandreparativefunction AT constantinsvonkaisenberg lowethanolconcentrationspromoteendothelialprogenitorcellcapacityandreparativefunction AT fraukevonversenhoynck lowethanolconcentrationspromoteendothelialprogenitorcellcapacityandreparativefunction |