Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells
Cellular heterogeneity presents an important challenge to the development of cell-based therapies where there is a fundamental requirement for predictable and reproducible outcomes. Transplanted Dental Pulp Stem/Progenitor Cells (DPSCs) have demonstrated early promise in experimental models of spina...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/1290561 |
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| author | Fraser I. Young Vsevolod Telezhkin Sarah J. Youde Martin S. Langley Maria Stack Paul J. Kemp Rachel J. Waddington Alastair J. Sloan Bing Song |
| author_facet | Fraser I. Young Vsevolod Telezhkin Sarah J. Youde Martin S. Langley Maria Stack Paul J. Kemp Rachel J. Waddington Alastair J. Sloan Bing Song |
| author_sort | Fraser I. Young |
| collection | DOAJ |
| description | Cellular heterogeneity presents an important challenge to the development of cell-based therapies where there is a fundamental requirement for predictable and reproducible outcomes. Transplanted Dental Pulp Stem/Progenitor Cells (DPSCs) have demonstrated early promise in experimental models of spinal cord injury and stroke, despite limited evidence of neuronal and glial-like differentiation after transplantation. Here, we report, for the first time, on the ability of single cell-derived clonal cultures of murine DPSCs to differentiate in vitro into immature neuronal-like and oligodendrocyte-like cells. Importantly, only DPSC clones with high nestin mRNA expression levels were found to successfully differentiate into Map2 and NF-positive neuronal-like cells. Neuronally differentiated DPSCs possessed a membrane capacitance comparable with primary cultured striatal neurons and small inward voltage-activated K+ but not outward Na+ currents were recorded suggesting a functionally immature phenotype. Similarly, only high nestin-expressing clones demonstrated the ability to adopt Olig1, Olig2, and MBP-positive immature oligodendrocyte-like phenotype. Together, these results demonstrate that appropriate markers may be used to provide an early indication of the suitability of a cell population for purposes where differentiation into a specific lineage may be beneficial and highlight that further understanding of heterogeneity within mixed cellular populations is required. |
| format | Article |
| id | doaj-art-6509d82a57d045c7a208ed29b28e0525 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-6509d82a57d045c7a208ed29b28e05252025-02-03T06:01:39ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/12905611290561Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor CellsFraser I. Young0Vsevolod Telezhkin1Sarah J. Youde2Martin S. Langley3Maria Stack4Paul J. Kemp5Rachel J. Waddington6Alastair J. Sloan7Bing Song8Oral and Biomedical Sciences, School of Dentistry, Cardiff University, Heath Park, Cardiff CF14 4XY, UKCardiff School of Biosciences, Cardiff University, Sir Martin Evans Building, Museum Avenue, Cardiff CF10 3AX, UKOral and Biomedical Sciences, School of Dentistry, Cardiff University, Heath Park, Cardiff CF14 4XY, UKOral and Biomedical Sciences, School of Dentistry, Cardiff University, Heath Park, Cardiff CF14 4XY, UKOral and Biomedical Sciences, School of Dentistry, Cardiff University, Heath Park, Cardiff CF14 4XY, UKCardiff School of Biosciences, Cardiff University, Sir Martin Evans Building, Museum Avenue, Cardiff CF10 3AX, UKOral and Biomedical Sciences, School of Dentistry, Cardiff University, Heath Park, Cardiff CF14 4XY, UKOral and Biomedical Sciences, School of Dentistry, Cardiff University, Heath Park, Cardiff CF14 4XY, UKOral and Biomedical Sciences, School of Dentistry, Cardiff University, Heath Park, Cardiff CF14 4XY, UKCellular heterogeneity presents an important challenge to the development of cell-based therapies where there is a fundamental requirement for predictable and reproducible outcomes. Transplanted Dental Pulp Stem/Progenitor Cells (DPSCs) have demonstrated early promise in experimental models of spinal cord injury and stroke, despite limited evidence of neuronal and glial-like differentiation after transplantation. Here, we report, for the first time, on the ability of single cell-derived clonal cultures of murine DPSCs to differentiate in vitro into immature neuronal-like and oligodendrocyte-like cells. Importantly, only DPSC clones with high nestin mRNA expression levels were found to successfully differentiate into Map2 and NF-positive neuronal-like cells. Neuronally differentiated DPSCs possessed a membrane capacitance comparable with primary cultured striatal neurons and small inward voltage-activated K+ but not outward Na+ currents were recorded suggesting a functionally immature phenotype. Similarly, only high nestin-expressing clones demonstrated the ability to adopt Olig1, Olig2, and MBP-positive immature oligodendrocyte-like phenotype. Together, these results demonstrate that appropriate markers may be used to provide an early indication of the suitability of a cell population for purposes where differentiation into a specific lineage may be beneficial and highlight that further understanding of heterogeneity within mixed cellular populations is required.http://dx.doi.org/10.1155/2016/1290561 |
| spellingShingle | Fraser I. Young Vsevolod Telezhkin Sarah J. Youde Martin S. Langley Maria Stack Paul J. Kemp Rachel J. Waddington Alastair J. Sloan Bing Song Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells Stem Cells International |
| title | Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells |
| title_full | Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells |
| title_fullStr | Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells |
| title_full_unstemmed | Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells |
| title_short | Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells |
| title_sort | clonal heterogeneity in the neuronal and glial differentiation of dental pulp stem progenitor cells |
| url | http://dx.doi.org/10.1155/2016/1290561 |
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