Assessing the antiproliferative effect of biogenic silver chloride nanoparticles on glioblastoma cell lines by quantitative image‐based analysis
Abstract Glioblastoma is the most life‐threatening tumour of the central nervous system. Temozolomide (TMZ) is the first‐choice oral drug for the treatment of glioblastoma, although it shows low efficacy. Silver nanoparticles (AgNPs) have been shown to exhibit biocidal activity in a variety of micro...
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Wiley
2021-08-01
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| Series: | IET Nanobiotechnology |
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| Online Access: | https://doi.org/10.1049/nbt2.12038 |
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| author | Nathalia Müller Mateus Eugenio Luciana F. Romão Jorge Marcondes de Souza Soniza V. Alves‐Leon Loraine Campanati Celso Sant’Anna |
| author_facet | Nathalia Müller Mateus Eugenio Luciana F. Romão Jorge Marcondes de Souza Soniza V. Alves‐Leon Loraine Campanati Celso Sant’Anna |
| author_sort | Nathalia Müller |
| collection | DOAJ |
| description | Abstract Glioblastoma is the most life‐threatening tumour of the central nervous system. Temozolomide (TMZ) is the first‐choice oral drug for the treatment of glioblastoma, although it shows low efficacy. Silver nanoparticles (AgNPs) have been shown to exhibit biocidal activity in a variety of microorganisms, including some pathogenic microorganisms. Herein, the antiproliferative effect of AgCl‐NPs on glioblastoma cell lines (GBM02 and GBM11) and on astrocytes was evaluated through automated quantitative image‐based analysis (HCA) of the cells. The cells were treated with 0.1‐5.0 μg/ml AgCl‐NPs or with 9.7‐48.5 μg/ml TMZ. Cells that received combined treatment were also analysed. At a maximum tested concentration of AgCl‐NPs, GBM02 and GBM11, the growth decreased by 93% and 40%, respectively, following 72 h of treatment. TMZ treatment decreased the proliferation of GBM02 and GBM11 cells by 58% and 34%, respectively. Combinations of AgCl‐NPs and TMZ showed intermediate antiproliferative effects; the lowest concentrations caused an inhibition similar to that obtained with TMZ, and the highest concentrations caused inhibition similar to that obtained with AgCl‐NPs alone. No significant changes in astrocyte proliferation were observed. The authors’ findings showed that HCA is a fast and reliable approach that can be used to evaluate the antiproliferative effect of the nanoparticles at the single‐cell level and that AgCl‐NPs are promising agents for glioblastoma treatment. |
| format | Article |
| id | doaj-art-64815b2e4a524c5d9d6fd29ec585f2bf |
| institution | Kabale University |
| issn | 1751-8741 1751-875X |
| language | English |
| publishDate | 2021-08-01 |
| publisher | Wiley |
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| series | IET Nanobiotechnology |
| spelling | doaj-art-64815b2e4a524c5d9d6fd29ec585f2bf2025-02-03T01:29:38ZengWileyIET Nanobiotechnology1751-87411751-875X2021-08-0115655856410.1049/nbt2.12038Assessing the antiproliferative effect of biogenic silver chloride nanoparticles on glioblastoma cell lines by quantitative image‐based analysisNathalia Müller0Mateus Eugenio1Luciana F. Romão2Jorge Marcondes de Souza3Soniza V. Alves‐Leon4Loraine Campanati5Celso Sant’Anna6Laboratory of Microscopy Applied to Life Science ‐ Lamav National Institute of Metrology Quality and Technology Duque de Caxias RJ BrazilLaboratory of Microscopy Applied to Life Science ‐ Lamav National Institute of Metrology Quality and Technology Duque de Caxias RJ BrazilLaboratory of Cellular Morphogenesis Federal University of Rio de Janeiro Rio de Janeiro RJ BrazilUniversity Hospital Clementino Fraga Filho Federal University of Rio de Janeiro Rio de Janeiro RJ BrazilUniversity Hospital Clementino Fraga Filho Federal University of Rio de Janeiro Rio de Janeiro RJ BrazilLaboratory of Cellular Morphogenesis Federal University of Rio de Janeiro Rio de Janeiro RJ BrazilLaboratory of Microscopy Applied to Life Science ‐ Lamav National Institute of Metrology Quality and Technology Duque de Caxias RJ BrazilAbstract Glioblastoma is the most life‐threatening tumour of the central nervous system. Temozolomide (TMZ) is the first‐choice oral drug for the treatment of glioblastoma, although it shows low efficacy. Silver nanoparticles (AgNPs) have been shown to exhibit biocidal activity in a variety of microorganisms, including some pathogenic microorganisms. Herein, the antiproliferative effect of AgCl‐NPs on glioblastoma cell lines (GBM02 and GBM11) and on astrocytes was evaluated through automated quantitative image‐based analysis (HCA) of the cells. The cells were treated with 0.1‐5.0 μg/ml AgCl‐NPs or with 9.7‐48.5 μg/ml TMZ. Cells that received combined treatment were also analysed. At a maximum tested concentration of AgCl‐NPs, GBM02 and GBM11, the growth decreased by 93% and 40%, respectively, following 72 h of treatment. TMZ treatment decreased the proliferation of GBM02 and GBM11 cells by 58% and 34%, respectively. Combinations of AgCl‐NPs and TMZ showed intermediate antiproliferative effects; the lowest concentrations caused an inhibition similar to that obtained with TMZ, and the highest concentrations caused inhibition similar to that obtained with AgCl‐NPs alone. No significant changes in astrocyte proliferation were observed. The authors’ findings showed that HCA is a fast and reliable approach that can be used to evaluate the antiproliferative effect of the nanoparticles at the single‐cell level and that AgCl‐NPs are promising agents for glioblastoma treatment.https://doi.org/10.1049/nbt2.12038antibacterial activitycancercellular biophysicsdrug delivery systemsmicroorganismsnanoparticles |
| spellingShingle | Nathalia Müller Mateus Eugenio Luciana F. Romão Jorge Marcondes de Souza Soniza V. Alves‐Leon Loraine Campanati Celso Sant’Anna Assessing the antiproliferative effect of biogenic silver chloride nanoparticles on glioblastoma cell lines by quantitative image‐based analysis IET Nanobiotechnology antibacterial activity cancer cellular biophysics drug delivery systems microorganisms nanoparticles |
| title | Assessing the antiproliferative effect of biogenic silver chloride nanoparticles on glioblastoma cell lines by quantitative image‐based analysis |
| title_full | Assessing the antiproliferative effect of biogenic silver chloride nanoparticles on glioblastoma cell lines by quantitative image‐based analysis |
| title_fullStr | Assessing the antiproliferative effect of biogenic silver chloride nanoparticles on glioblastoma cell lines by quantitative image‐based analysis |
| title_full_unstemmed | Assessing the antiproliferative effect of biogenic silver chloride nanoparticles on glioblastoma cell lines by quantitative image‐based analysis |
| title_short | Assessing the antiproliferative effect of biogenic silver chloride nanoparticles on glioblastoma cell lines by quantitative image‐based analysis |
| title_sort | assessing the antiproliferative effect of biogenic silver chloride nanoparticles on glioblastoma cell lines by quantitative image based analysis |
| topic | antibacterial activity cancer cellular biophysics drug delivery systems microorganisms nanoparticles |
| url | https://doi.org/10.1049/nbt2.12038 |
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