Absence of Rybp Compromises Neural Differentiation of Embryonic Stem Cells
Rybp (Ring1 and Yy1 Binding Protein) is a transcriptional regulator and member of the noncanonical polycomb repressive complex 1 with essential role in early embryonic development. We have previously described that alteration of Rybp dosage in mouse models induced striking neural tube defects (NTDs)...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/4034620 |
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| author | Gergo Kovacs Viktoria Szabo Melinda K. Pirity |
| author_facet | Gergo Kovacs Viktoria Szabo Melinda K. Pirity |
| author_sort | Gergo Kovacs |
| collection | DOAJ |
| description | Rybp (Ring1 and Yy1 Binding Protein) is a transcriptional regulator and member of the noncanonical polycomb repressive complex 1 with essential role in early embryonic development. We have previously described that alteration of Rybp dosage in mouse models induced striking neural tube defects (NTDs), exencephaly, and disorganized neurocortex. In this study we further investigated the role of Rybp in neural differentiation by utilising wild type (rybp+/+) and rybp null mutant (rybp-/-) embryonic stem cells (ESCs) and tried to uncover underlying molecular events that are responsible for the observed phenotypic changes. We found that rybp null mutant ESCs formed less matured neurons, astrocytes, and oligodendrocytes from existing progenitors than wild type cells. Furthermore, lack of rybp coincided with altered gene expression of key neural markers including Pax6 and Plagl1 pinpointing a possible transcriptional circuit among these genes. |
| format | Article |
| id | doaj-art-643b3f79c5fc45eeba5a14994d0d686b |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-643b3f79c5fc45eeba5a14994d0d686b2025-02-03T01:11:02ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/40346204034620Absence of Rybp Compromises Neural Differentiation of Embryonic Stem CellsGergo Kovacs0Viktoria Szabo1Melinda K. Pirity2Institute of Genetics, Laboratory of Embryonic and Induced Pluripotent Stem Cells, Biological Research Centre, Hungarian Academy of Sciences, Temesvári krt. 62, Szeged 6726, HungaryInstitute of Genetics, Laboratory of Embryonic and Induced Pluripotent Stem Cells, Biological Research Centre, Hungarian Academy of Sciences, Temesvári krt. 62, Szeged 6726, HungaryInstitute of Genetics, Laboratory of Embryonic and Induced Pluripotent Stem Cells, Biological Research Centre, Hungarian Academy of Sciences, Temesvári krt. 62, Szeged 6726, HungaryRybp (Ring1 and Yy1 Binding Protein) is a transcriptional regulator and member of the noncanonical polycomb repressive complex 1 with essential role in early embryonic development. We have previously described that alteration of Rybp dosage in mouse models induced striking neural tube defects (NTDs), exencephaly, and disorganized neurocortex. In this study we further investigated the role of Rybp in neural differentiation by utilising wild type (rybp+/+) and rybp null mutant (rybp-/-) embryonic stem cells (ESCs) and tried to uncover underlying molecular events that are responsible for the observed phenotypic changes. We found that rybp null mutant ESCs formed less matured neurons, astrocytes, and oligodendrocytes from existing progenitors than wild type cells. Furthermore, lack of rybp coincided with altered gene expression of key neural markers including Pax6 and Plagl1 pinpointing a possible transcriptional circuit among these genes.http://dx.doi.org/10.1155/2016/4034620 |
| spellingShingle | Gergo Kovacs Viktoria Szabo Melinda K. Pirity Absence of Rybp Compromises Neural Differentiation of Embryonic Stem Cells Stem Cells International |
| title | Absence of Rybp Compromises Neural Differentiation of Embryonic Stem Cells |
| title_full | Absence of Rybp Compromises Neural Differentiation of Embryonic Stem Cells |
| title_fullStr | Absence of Rybp Compromises Neural Differentiation of Embryonic Stem Cells |
| title_full_unstemmed | Absence of Rybp Compromises Neural Differentiation of Embryonic Stem Cells |
| title_short | Absence of Rybp Compromises Neural Differentiation of Embryonic Stem Cells |
| title_sort | absence of rybp compromises neural differentiation of embryonic stem cells |
| url | http://dx.doi.org/10.1155/2016/4034620 |
| work_keys_str_mv | AT gergokovacs absenceofrybpcompromisesneuraldifferentiationofembryonicstemcells AT viktoriaszabo absenceofrybpcompromisesneuraldifferentiationofembryonicstemcells AT melindakpirity absenceofrybpcompromisesneuraldifferentiationofembryonicstemcells |