Rapid lightsheet fluorescence imaging of whole Drosophila brains at nanoscale resolution by potassium acrylate-based expansion microscopy

Rapid lightsheet fluorescence imaging of whole Drosophila brains at nanoscale resolution by potassium acrylate-based expansion microscopy

Abstract Taking advantage of the good mechanical strength of expanded Drosophila brains and to tackle their relatively large size that can complicate imaging, we apply potassium (poly)acrylate-based hydrogels for expansion microscopy (ExM), resulting in a 40x plus increased resolution of transgenic...

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Main Authors: Xuejiao Tian, Tzu-Yang Lin, Po-Ting Lin, Min-Ju Tsai, Hsin Chen, Wen-Jie Chen, Chia-Ming Lee, Chiao-Hui Tu, Jui-Cheng Hsu, Tung-Han Hsieh, Yi-Chung Tung, Chien-Kai Wang, Suewei Lin, Li-An Chu, Fan-Gang Tseng, Yi-Ping Hsueh, Chi-Hon Lee, Peilin Chen, Bi-Chang Chen
Format: Article
Language:English
Published: Nature Portfolio 2024-12-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-024-55305-8
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https://doi.org/10.1038/s41467-024-55305-8

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