Diagnostic Application of IS900 PCR Using Blood as a Source Sample for the Detection of Mycobacterium avium Subspecies Paratuberculosis in Early and Subclinical Cases of Caprine Paratuberculosis
Efficacy of IS900 blood PCR was evaluated for the presence of MAP infection. Serum, fecal, and blood samples of kids, young, and adult goats from farm and farmer's herds in Mathura district were also screened by ELISA, microscopy and culture. Of 111 goats (kids: 40, young: 14, adults: 57) scree...
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Wiley
2010-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.4061/2010/748621 |
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| author | P. K. Singh S. V. Singh H. Kumar J. S. Sohal A. V. Singh |
| author_facet | P. K. Singh S. V. Singh H. Kumar J. S. Sohal A. V. Singh |
| author_sort | P. K. Singh |
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| description | Efficacy of IS900 blood PCR was evaluated for the presence of MAP infection. Serum, fecal, and blood samples of kids, young, and adult goats from farm and farmer's herds in Mathura district were also screened by ELISA, microscopy and culture. Of 111 goats (kids: 40, young: 14, adults: 57) screened, 77.5% were positive by blood PCR. Of 76
goats, 90.8% (kids: 87.5% and adults: 94.4%) were positive by PCR. From 21 kids and
14 young goats, 42.8 and 57.1% were positive. gDNA from goats was genotyped as MAP
“Indian Bison type”. Of 21 fecal samples of kids examined by microscopy, 66.7% were
positive. In ELISA, 9.5 and 57.1% kids were positives as “type I” and “type II” reactors,
respectively. Screening 14 young goats by culture of blood clots, 28.6% were positive.
Agreement was substantial between PCR and microscopy. It was fair and moderate when
PCR and microscopy were compared with type I and type II reactors, respectively.
Presence of MAP in non-clinical kids and young goats indicate early or subclinical
infection. Blood PCR was rapid, sensitive, and specific assay for detection of MAP in
any stage (early, subclinical, and clinical) and age (kids, young, and adult) of goats. |
| format | Article |
| id | doaj-art-639af6d15c0e49adb3ac5b096aa67713 |
| institution | Kabale University |
| issn | 2042-0048 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-639af6d15c0e49adb3ac5b096aa677132025-02-03T06:01:02ZengWileyVeterinary Medicine International2042-00482010-01-01201010.4061/2010/748621748621Diagnostic Application of IS900 PCR Using Blood as a Source Sample for the Detection of Mycobacterium avium Subspecies Paratuberculosis in Early and Subclinical Cases of Caprine ParatuberculosisP. K. Singh0S. V. Singh1H. Kumar2J. S. Sohal3A. V. Singh4Microbiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, PO - Farah, Mathura (UP), Uttar Pradesh 281 122, IndiaMicrobiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, PO - Farah, Mathura (UP), Uttar Pradesh 281 122, IndiaMicrobiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, PO - Farah, Mathura (UP), Uttar Pradesh 281 122, IndiaMicrobiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, PO - Farah, Mathura (UP), Uttar Pradesh 281 122, IndiaMicrobiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, PO - Farah, Mathura (UP), Uttar Pradesh 281 122, IndiaEfficacy of IS900 blood PCR was evaluated for the presence of MAP infection. Serum, fecal, and blood samples of kids, young, and adult goats from farm and farmer's herds in Mathura district were also screened by ELISA, microscopy and culture. Of 111 goats (kids: 40, young: 14, adults: 57) screened, 77.5% were positive by blood PCR. Of 76 goats, 90.8% (kids: 87.5% and adults: 94.4%) were positive by PCR. From 21 kids and 14 young goats, 42.8 and 57.1% were positive. gDNA from goats was genotyped as MAP “Indian Bison type”. Of 21 fecal samples of kids examined by microscopy, 66.7% were positive. In ELISA, 9.5 and 57.1% kids were positives as “type I” and “type II” reactors, respectively. Screening 14 young goats by culture of blood clots, 28.6% were positive. Agreement was substantial between PCR and microscopy. It was fair and moderate when PCR and microscopy were compared with type I and type II reactors, respectively. Presence of MAP in non-clinical kids and young goats indicate early or subclinical infection. Blood PCR was rapid, sensitive, and specific assay for detection of MAP in any stage (early, subclinical, and clinical) and age (kids, young, and adult) of goats.http://dx.doi.org/10.4061/2010/748621 |
| spellingShingle | P. K. Singh S. V. Singh H. Kumar J. S. Sohal A. V. Singh Diagnostic Application of IS900 PCR Using Blood as a Source Sample for the Detection of Mycobacterium avium Subspecies Paratuberculosis in Early and Subclinical Cases of Caprine Paratuberculosis Veterinary Medicine International |
| title | Diagnostic Application of IS900 PCR Using Blood as a Source Sample for the Detection of Mycobacterium avium Subspecies Paratuberculosis in Early and Subclinical Cases of Caprine Paratuberculosis |
| title_full | Diagnostic Application of IS900 PCR Using Blood as a Source Sample for the Detection of Mycobacterium avium Subspecies Paratuberculosis in Early and Subclinical Cases of Caprine Paratuberculosis |
| title_fullStr | Diagnostic Application of IS900 PCR Using Blood as a Source Sample for the Detection of Mycobacterium avium Subspecies Paratuberculosis in Early and Subclinical Cases of Caprine Paratuberculosis |
| title_full_unstemmed | Diagnostic Application of IS900 PCR Using Blood as a Source Sample for the Detection of Mycobacterium avium Subspecies Paratuberculosis in Early and Subclinical Cases of Caprine Paratuberculosis |
| title_short | Diagnostic Application of IS900 PCR Using Blood as a Source Sample for the Detection of Mycobacterium avium Subspecies Paratuberculosis in Early and Subclinical Cases of Caprine Paratuberculosis |
| title_sort | diagnostic application of is900 pcr using blood as a source sample for the detection of mycobacterium avium subspecies paratuberculosis in early and subclinical cases of caprine paratuberculosis |
| url | http://dx.doi.org/10.4061/2010/748621 |
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