Epigenetic Application of ATAC-Seq Based on Tn5 Transposase Purification Technology
Background. Assays of transposase accessible chromatin sequencing (ATAC-seq) is an efficient assay to investigate chromatin accessibility, which depends on the activity of a robust Tn5 transposase to fragment the genome while cutting in the sequencing adapters. Methods. We propose reliable approache...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Genetics Research |
| Online Access: | http://dx.doi.org/10.1155/2022/8429207 |
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| author | Wangchun Li U Tim Wu Yu Cheng Yanhao Huang Lipeng Mao Menghan Sun Congling Qiu Lin Zhou Lijuan Gao |
| author_facet | Wangchun Li U Tim Wu Yu Cheng Yanhao Huang Lipeng Mao Menghan Sun Congling Qiu Lin Zhou Lijuan Gao |
| author_sort | Wangchun Li |
| collection | DOAJ |
| description | Background. Assays of transposase accessible chromatin sequencing (ATAC-seq) is an efficient assay to investigate chromatin accessibility, which depends on the activity of a robust Tn5 transposase to fragment the genome while cutting in the sequencing adapters. Methods. We propose reliable approaches for purifying hyperactive Tn5 transposase by chitin magnetic bead sorting. Double-stranded DNA of J76 cells and 293T cells were digested and subjected to tagmentation as test samples with Tn5 transposase, and libraries were established and sequenced. Sequencing data was then analyzed for peak calling, GO enrichment, and motif analysis. Results. We report a set of rapid, efficient, and low-cost methods for ATAC-seq library construction and data analysis, through large-scale and rapid sequencing. These methods can provide a reference for the study of epigenetic regulation of gene expression. |
| format | Article |
| id | doaj-art-62bba7a56b764776a3d8b74002b6b29b |
| institution | Kabale University |
| issn | 1469-5073 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Genetics Research |
| spelling | doaj-art-62bba7a56b764776a3d8b74002b6b29b2025-02-03T01:23:12ZengWileyGenetics Research1469-50732022-01-01202210.1155/2022/8429207Epigenetic Application of ATAC-Seq Based on Tn5 Transposase Purification TechnologyWangchun Li0U Tim Wu1Yu Cheng2Yanhao Huang3Lipeng Mao4Menghan Sun5Congling Qiu6Lin Zhou7Lijuan Gao8Department of Critical Care MedicineMeng Yi Center LimitedInstitute of Geriatric ImmunologyInstitute of Geriatric ImmunologyInstitute of Geriatric ImmunologyInstitute of Geriatric ImmunologyDepartment of Microbiology and ImmunologyNanjing Maternity and Child Health Care HospitalInstitute of Geriatric ImmunologyBackground. Assays of transposase accessible chromatin sequencing (ATAC-seq) is an efficient assay to investigate chromatin accessibility, which depends on the activity of a robust Tn5 transposase to fragment the genome while cutting in the sequencing adapters. Methods. We propose reliable approaches for purifying hyperactive Tn5 transposase by chitin magnetic bead sorting. Double-stranded DNA of J76 cells and 293T cells were digested and subjected to tagmentation as test samples with Tn5 transposase, and libraries were established and sequenced. Sequencing data was then analyzed for peak calling, GO enrichment, and motif analysis. Results. We report a set of rapid, efficient, and low-cost methods for ATAC-seq library construction and data analysis, through large-scale and rapid sequencing. These methods can provide a reference for the study of epigenetic regulation of gene expression.http://dx.doi.org/10.1155/2022/8429207 |
| spellingShingle | Wangchun Li U Tim Wu Yu Cheng Yanhao Huang Lipeng Mao Menghan Sun Congling Qiu Lin Zhou Lijuan Gao Epigenetic Application of ATAC-Seq Based on Tn5 Transposase Purification Technology Genetics Research |
| title | Epigenetic Application of ATAC-Seq Based on Tn5 Transposase Purification Technology |
| title_full | Epigenetic Application of ATAC-Seq Based on Tn5 Transposase Purification Technology |
| title_fullStr | Epigenetic Application of ATAC-Seq Based on Tn5 Transposase Purification Technology |
| title_full_unstemmed | Epigenetic Application of ATAC-Seq Based on Tn5 Transposase Purification Technology |
| title_short | Epigenetic Application of ATAC-Seq Based on Tn5 Transposase Purification Technology |
| title_sort | epigenetic application of atac seq based on tn5 transposase purification technology |
| url | http://dx.doi.org/10.1155/2022/8429207 |
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